Antimicrobial Homoisoflavonoids from the Rhizomes of <i>Polygonatum verticillatum</i>

Sharma, S.K.; Patial, Vijeta; Singh, Dharam; Sharma, Upendra; Kumar, Dinesh

doi:10.1002/cbdv.201800430

Cited by 14 publications

(8 citation statements)

References 18 publications

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“…Homoisoflavonoids were isolated from the rhizomes of P. verticillatum as described by Sharma et al . Quercetin, kaempferol, rutin, ascorbic acid and DPPH (2,2‐diphenyl‐1‐picrylhydrazyl) were procured from Sigma Aldrich (St Louis, MO, USA).…”

Section: Methodsmentioning

confidence: 99%

“…Rutin, quercetin and kaempherol ( 1–3 ) were detected and confirmed using ultrahigh‐performance liquid chromatography tandem mass spectrometry (UHPLC‐MS/MS) in P. verticillatum . Homoisoflavonoids ( 4–6 ) were isolated from the rhizomes of P. verticillatum at CSIR‐IHBT, Palampur, India as previously described …”

Section: Methodsmentioning

confidence: 99%

“…Homoisoflavonoids were isolated from the rhizomes of P. verticillatum as described by Sharma et al 15 Quercetin, kaempferol, rutin, ascorbic acid and DPPH (2,2-diphenyl-1-picrylhydrazyl) were procured from Sigma Aldrich (St Louis, MO, USA). Analytical reagents and solvents were purchased from John Townsend Baker (Mallinckrodt Baker Inc., St Louis, MO, USA) and S. D. Fine Chemicals Ltd (Mumbai, India), respectively.…”

Section: Reagent and Materialsmentioning

confidence: 99%

“…Homoisoflavonoids (4)(5)(6) were isolated from the rhizomes of P. verticillatum at CSIR-IHBT, Palampur, India as previously described. 15…”

Section: Selection Of Chemical Markers As Standardsmentioning

confidence: 99%

“…The plant is considered as rasayana and a vital ingredient of ayurvedic products because of its health promoting potentials via cell regeneration properties . Earlier reports described only a few molecules including β ‐sitosterol, lectins, diosgenin, 5‐hydroxymethyl‐2‐furaldehyde, santonin, 2‐hydroxybenzoic acid, homoisoflavonoids and polysaccharides from P. verticillatum . However, P. verticillatum has abundant demand in the global market but knowledge of the chemistry and a lack of reliable analytical methods can lead to the use of substitution and adulteration .…”

Section: Introductionmentioning

confidence: 99%

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Quantitative analysis of flavonols, flavonol glycoside and homoisoflavonoids in Polygonatum verticillatum using UHPLC‐DAD‐QTOF‐IMS and evaluation of their antioxidant potential

Sharma

Joshi

Kumar

2020

Phytochemical Analysis

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Introduction Polygonatum is widely used as a part of food in different regions of the world which covers five main categories such as drinks, vegetables, snacks, staple and seasoning foods. Presently, no analytical method is available for the quality control of Polygonatum. Objective Development and validation of a method using ultrahigh‐performance liquid chromatography diode array detector quadrupole time‐of‐flight (UHPLC‐DAD/QTOF) technique for the estimation of six compounds including a flavonol glycoside [rutin (1)], two flavonols [quercetin (2) and kaempherol (3)] and three homoisoflavonoids [5,7‐dihydroxy‐3‐(2‐hydroxy‐4‐methoxybenzyl)‐chroman‐4‐one (4), 5,7‐dihydroxy‐3‐(2‐hydroxy‐4‐methoxybenzyl)‐8‐methylchroman‐4‐one (5) and 5,7‐dihydroxy‐3‐(4‐methoxybenzyl)‐8‐methylchroman‐4‐one (6)]. In addition, screening of extract, fractions and compounds of P. verticillatum for antioxidant activity was also determined. Methodology The separation was achieved on C‐18 column using acetonitrile and water containing 0.1% formic acid. The method was validated as per ICH (International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use) guidelines. The validated method was applied for the simultaneous identification and quantification of compounds 1–6 in extract (E) and fractions (F1–F4) of P. verticillatum. Furthermore, antioxidant potential of E, F1 and F2 and compounds was evaluated using DPPH (2,2‐diphenyl‐1‐picrylhydrazyl) assay. Results The method was within the linear range (r2) of 0.982 to 0.999, precise (intra‐ and inter‐day percentage relative standard deviations < 2.72 and 2.26) and accurate with recoveries (89.1–98.3%). The limit of detection and limit of quantification were in the ranges 0.02–0.16 and 0.06–0.48 ng/mL, respectively. Compounds 1–6 were quantified in all the samples. Compounds 1, 2 and 5 showed higher activity with half maximal inhibitory concentration (IC50) values 0.41, 0.39, 0.72 at 10, 20 and 30 μg/mL, respectively. Conclusion Developed method will be helpful to assess the quality of P. verticillatum raw material and their derived products.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Reagent and Materialsmentioning

confidence: 99%

“…Homoisoflavonoids (4)(5)(6) were isolated from the rhizomes of P. verticillatum at CSIR-IHBT, Palampur, India as previously described. 15…”

Section: Selection Of Chemical Markers As Standardsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Quantitative analysis of flavonols, flavonol glycoside and homoisoflavonoids in Polygonatum verticillatum using UHPLC‐DAD‐QTOF‐IMS and evaluation of their antioxidant potential

Sharma

Joshi

Kumar

2020

Phytochemical Analysis

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Cytotoxic steroidal compounds, enriched fraction and extract of Trillium govanianum against HCT‐116 cell line

Kumar,

Kapoor,

Padwad

et al. 2024

Vietnam Journal of Chemistry

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Trillium govanianum is a source of steroidal compounds and is used for the treatment of sexual disorders, dysentery, cancer, and inflammation. In the current investigation, steroidal compounds 1–5 (20‐hydroxyecdysone [1], pennogenin‐3‐O‐β‐chacotrioside [2], dioscin [3], trillin [4], diosgenin [5]) were isolated, including dioscin and trillin, as a new report from this species. Further, extract (TGE), steroids enriched fraction (TGEF) and 1–5 were evaluated for cytotoxicity against the HCT‐116 cell line. Cytotoxicity activity was found to be significant in samples and might be due to the generation of intracellular reactive oxygen species production. TGE and TGEF showed maximum inhibition (>92%) at 25 and 5 µg/mL in 72 h, while 2 and 3 showed better activity (>95%) at 5.65 and 11.51 µM in 72 h than the vinblastine drug (20 µM). Furthermore, the ultra‐performance liquid chromatography‐photo diode array method was validated for a determination of 1–5. Compounds 1–3, and 5 were quantified in TGE and TGEF while 4 was only identified. The findings revealed that activity of TGE and TGEF, might be due to compounds 2 (49.04 ± 2.22 and 77.40 ± 0.72 mg/g) and 3 (230.88 ± 7.07 and 584.67 ± 5.34 mg/g), respectively. The study also suggested that 2 and 3 can be a drug molecule as vinblastine while TGEF may be developed as a phytopharmaceutical drug for colon cancer treatment.

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Chromanone-A Prerogative Therapeutic Scaffold: An Overview

Kamboj

Singh

2021

Arab J Sci Eng

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Chromanone or Chroman-4-one is the most important and interesting heterobicyclic compound and acts as a building block in medicinal chemistry for isolation, designing and synthesis of novel lead compounds. Structurally, absence of a double bond in chromanone between C-2 and C-3 shows a minor difference from chromone but exhibits significant variations in biological activities. In the present review, various studies published on synthesis, pharmacological evaluation on chroman-4-one analogues are addressed to signify the importance of chromanone as a versatile scaffold exhibiting a wide range of pharmacological activities. But, due to poor yield in the case of chemical synthesis and expensive isolation procedure from natural compounds, more studies are required to provide the most effective and cost-effective methods to synthesize novel chromanone analogs to give leads to chemistry community. Considering the versatility of chromanone, this review is designed to impart comprehensive, critical and authoritative information about chromanone template in drug designing and development.

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Antimicrobial Homoisoflavonoids from the Rhizomes of Polygonatum verticillatum

Cited by 14 publications

References 18 publications

Quantitative analysis of flavonols, flavonol glycoside and homoisoflavonoids in Polygonatum verticillatum using UHPLC‐DAD‐QTOF‐IMS and evaluation of their antioxidant potential

Quantitative analysis of flavonols, flavonol glycoside and homoisoflavonoids in Polygonatum verticillatum using UHPLC‐DAD‐QTOF‐IMS and evaluation of their antioxidant potential

Cytotoxic steroidal compounds, enriched fraction and extract of Trillium govanianum against HCT‐116 cell line

Chromanone-A Prerogative Therapeutic Scaffold: An Overview

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