Antimicrobial pentacyclic triterpenes and glycosides from the stem bark ofCussonia bancoensis

Abstract

The stem bark of Cussonia bancoensis is used traditionally for the treatment of infection and pain. A bioassay guided fractionation of the methanol stem bark extract led to the isolation of five pentacyclic triterpenes and glycosides identified based on spectroscopic data as 23-hydroxyursolic acid (CB1), hederagenin (CB2), 3-O-α-L-arabinopyranosyl-echinocystic acid (CB3), 3-O-α-L-arabinopyranosyloleanolic acid (CB4) and 3-O-α-L-arabinopyranosyl-ursolic acid (CB5). CB2 -CB5 are being reported for the first time from this species. The compounds were evaluated for antimicrobial activity against ten microorganisms using the HT-SPOTi method. CB3 demonstrated remarkable antimicrobial activity against S. aureus, S. pyogens, E. faecalis, S. typhi and C. albicans at MICs between 3.12 and 12.5 µg/mL. Among the studied compounds, it was observed that hydroxylation of position C-16 of the oleanane skeleton may enhance antimicrobial activity. This study gives insight into the anti-infective constituents of the stem bark of C. bancoensis and justifies its use in ethnomedicine.

Role of Herbs and Medicinal Spices as Modulators of Gut Microbiota

“…Likewise, triterpene 1α, 3β, and 23-trihydroxyolean-12-in-29-oic acid isolated Combretum imberbe which inhibited the in vitro growth of Mycobacterium fortuitum and Staphylococcus aureus [81]. In an in vitro study, triterpenes such as 23-hydroxyursolic acid, hederagenin, 3-O-α-L-arabinopyranosyl-echinocystic acid, 3-O-α-L-arabinopyranosyl-oleanolic acid, and 3-O-α-L-arabinopyranosyl-ursolic acid isolated from the stem bark of Cussonia bancoensis stopped the growth of Staphylococcus aureus, Streptococcus pyogenes, Enterococcus faecalis, Salmonella typhi, and Candida albicans [71] ( Table 1).…”