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The metabolites profile of C. fruticosa (L.) A. Chev. leaves, 12 isolates, and its nephroprotective and hepatoprotective activities are described.

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Sansevieria species show various bioactivities. Nevertheless, its therapeutic prospect in liver fibrosis even now is uninvestigated. The present study was conducted to analyze the metabolomic profile of Sansevieria trifasciata hort ex. Prain leaves and roots via HPLC-PAD-ESI/MS and evaluation of its hepatoprotective effect. The identified phytoconstituents were mainly steroidal saponins, phenolics and terpenoids. Sixty-one compounds were tentatively identified in StLE and fifty-nine compounds in StRE. Thioacetamide-induced liver fibrosis rat model was used to evaluate the hepatoprotective effect of Sansevieria trifasciata extracts via activation of the NRF2/ARE signaling pathway. Measurements of serum alanine transaminase (ALT), aspartate transaminase (AST) and malondialdehyde (MDA) were significantly decreased in treated groups with StLE and StRE (at doses of 200 and 100 mg/kg/day) compared with the TAA group. Also, the levels of reduced glutathione (GSH) content and hepatic mRNA levels of Nrf2, HO-1, NQO-1 and Keap-1 were markedly elevated. The prominent hepatoprotective effect was shown in StRE treated groups. Histological findings further confirmed the protective role of the plant against TAA-induced liver fibrosis. In conclusion, the abovementioned results indicated that the hepatoprotective mechanism of StLE and StRE could be achieved by activating Nrf2-ARE signaling pathways to alleviate oxidative stress and inflammation.

Column chromatography of the stem aqueous methanolic extract of Dracaena reflexa Lam. (DRSE) led to the isolation of five flavonoids, one phenolic glycoside, one triterpenoid and two steroidal saponins. Furthermore, 44 compounds were tentatively identified in the phytoconstituent profile of DRSE using HPLC-ESI-MS/MS. The antioxidant activity of DRSE was evaluated. In a DPPH radical scavenging assay, DRSE exhibited an IC 50 value of 311.6 ± 10.10 μg/ml compared with the IC 50 value of the standard Trolox (24.42 ± 0.87 μg/ml). The antioxidant activities of DRSE using ABTS assay and ferric reducing antioxidant power assay were 326.63 μM Trolox equivalents/mg extract and 208.67 μM Trolox equivalents/mg extract, respectively.The wound-healing activity of DRSE was studied by the scratch assay using Human Skin Fibroblast cells. After 24 h DRSE (at 10 and 20 μg/ml) decreased the wound width to 0.55 ± 0.37 and 0.47 ± 0.55 mm, respectively, compared with the wound width in the control cells (0.77 ± 0.17 mm). This result suggested that DRSE improved the wound-healing process by inducing the migration of fibroblasts.Moreover, a docking study was performed to evaluate the binding affinity of the identified phytoconstituents toward GSK-3β relative to the co-crystalized inhibitor and curcumin with the possible involvement of this pathway in the wound-healing activity of the extract.