Antimicrobial peptide coatings for hydroxyapatite: electrostatic and covalent attachment of antimicrobial peptides to surfaces

Townsend, Leigh; Williams, Richard L.; Anuforom, Olachi; Berwick, Matthew R.; Halstead, Fenella D.; Hughes, Erik A. B.; Stamboulis, Artemis; Oppenheim, Beryl; Gough, Julie E.; Grover, Liam M.; Scott, Richard A.; Webber, Mark A.; Peacock, Anna F. A.; Belli, Antonio; Logan, Ann; Cogan, Felicity de

doi:10.1098/rsif.2016.0657

Cited by 48 publications

(41 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The authors demonstrated that after 3 weeks, since the infection initiation and 12 weeks of treatment with vancomycin loaded nanoHAp/collagen/calcium sulfate composite, no evidence of infection was observed, while the formation of new trabecular bone was noticed at the implantation site. Furthermore, Townsend et al [72] accomplished by a dual approach-covalently bonding of antimicrobial peptide (AMP) to HAp, followed by deposition of electrostatically bound AMPs-an effective coating design, which was proven to be stable for a period of over 12 months and had the potential to prevent implant colonization by both Gram-positive (S. aureus and Staphylococcus epidermidis) and Gram-negative (P. aeruginosa) bacteria.…”

Section: Resultsmentioning

confidence: 99%

Dextran-Thyme Magnesium-Doped Hydroxyapatite Composite Antimicrobial Coatings

et al. 2020

View full text Add to dashboard Cite

The dextran-thyme magnesium-doped hydroxyapatite (10MgHAp-Dex-thyme) composite layers were prepared by a dip-coating procedure from stable suspensions and further analyzed for the first time. Different characterization techniques were employed to explore the physical-chemical features of the 10MgHAp-Dex-thyme suspensions and derived coatings. Information regarding the 10MgHAp-Dex-thyme suspensions was extracted on the basis of dynamic light scattering, zeta potential, and ultrasound measurements. The crystalline quality of the biocomposite powders—resulting after the centrifugation of suspensions—and the layers deposited on glass was assessed by X-ray diffraction in symmetric and grazing incidence geometries, respectively. The chemical structure and presence of functional groups were evaluated for both powder and coating by Fourier transform infrared spectroscopy in attenuated total reflectance mode. The extent of the antimicrobial effect range of the biocomposite suspensions and coatings was tested against different Gram-positive and Gram-negative bacteria (Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, and Pseudomonas aeruginosa) and fungus (Candida albicans) strains with promising results.

show abstract

Section: Resultsmentioning

confidence: 99%

Dextran-Thyme Magnesium-Doped Hydroxyapatite Composite Antimicrobial Coatings

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Silver (Jones et al, 2006) quaternary ammonium compounds (Ravikumar et al, 2006), salicylic acid (Bryers et al, 2006) or polymeric substances (Siedenbiedel and Tiller, 2012) are highly effective in vitro, but use of these chemicals can induce toxicity in vivo (Ramstedt et al, 2009). Loss of activity and the inability to sterilize surfaces once antimicrobials are covalently bound to devices are also problems associated with antimicrobial coatings (Vasilev et al, 2009;Townsend et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Mechanism of Action of Surface Immobilized Antimicrobial Peptides Against Pseudomonas aeruginosa

et al. 2020

View full text Add to dashboard Cite

Bacterial colonization and biofilm development on medical devices can lead to infection. Antimicrobial peptide-coated surfaces may prevent such infections. Melimine and Mel4 are chimeric cationic peptides showing broad-spectrum antimicrobial activity once attached to biomaterials and are highly biocompatible in animal models and have been tested in Phase I and II/III human clinical trials. These peptides were covalently attached to glass using an azidobenzoic acid linker. Peptide attachment was confirmed using X-ray photoelectron spectroscopy and amino acid analysis. Mel4 when bound to glass was able to adopt a more ordered structure in the presence of bacterial membrane mimetic lipids. The ability of surface bound peptides to neutralize endotoxin was measured along with their interactions with the bacterial cytoplasmic membrane which were analyzed using DiSC(3)-5 and Sytox green, Syto-9, and PI dyes with fluorescence microscopy. Leakage of ATP and nucleic acids from cells were determined by analyzing the surrounding fluid. Attachment of the peptides resulted in increases in the percentage of nitrogen by 3.0% and 2.4%, and amino acid concentrations to 0.237 nmole and 0.298 nmole per coverslip on melimine and Mel4 coated surfaces, respectively. The immobilized peptides bound lipopolysaccharide and disrupted the cytoplasmic membrane potential of Pseudomonas aeruginosa within 15 min. Membrane depolarization was associated with a reduction in bacterial viability by 82% and 63% for coatings melimine and Mel4, respectively (p < 0.001). Disruption of membrane potential was followed by leakage of ATP from melimine (1.5 ± 0.4 nM) or Mel4 (1.3 ± 0.2 nM) coated surfaces compared to uncoated glass after 2 h (p < 0.001). Sytox green influx started after 3 h incubation with either peptide. Melimine coatings yielded 59% and Mel4 gave 36% PI stained cells after 4 h. Release of the larger molecules (DNA/RNA) commenced after 4 h for melimine (1.8 ± 0.9 times more than control; p = 0.008) and after 6 h with Mel4 (2.1 ± 0.2 times more than control; p < 0.001). The mechanism of action of surface bound melimine and Mel4 was similar to that of the peptides in solution, however, their immobilization resulted in much slower (approximately 30 times) kinetics.

show abstract

“…Thanks to many years of research, protease inhibitors have gained the status of registered drugs and have been successfully used in the treatment of diseases caused by HIV, influenza, or HCV virus [30][31][32]. Currently, many biologically active substances covalently immobilized on the surface of biomaterials are known [9,[12][13][14][15][16][17][33][34][35]. Therefore, we decided to perform covalent immobilization of three serine protease inhibitors, one synthetic (AEBSF) and two natural (soy inhibitor and α 1 -antitrypsin), on the surface of biomaterials that are commonly used in regenerative medicine.…”

Section: Introductionmentioning

confidence: 99%

Serine Protease Inhibitors—New Molecules for Modification of Polymeric Biomaterials

et al. 2020

View full text Add to dashboard Cite

Three serine protease inhibitors (AEBSF, soy inhibitor, α1-antitrypsin) were covalently immobilized on the surface of three polymer prostheses with the optimized method. The immobilization efficiency ranged from 11 to 51%, depending on the chosen inhibitor and biomaterial. The highest activity for all inhibitors was observed in the case of immobilization on the surface of the polyester Uni-Graft prosthesis, and the preparations obtained showed high stability in the environment with different pH and temperature values. Modification of the Uni-Graft prosthesis surface with the synthetic AEBSF inhibitor and human α1-antitrypsin inhibited the adhesion and multiplication of Staphylococcus aureus subs. aureus ATCC® 25923TM and Candida albicans from the collection of the Department of Genetics and Microbiology, UMCS. Optical profilometry analysis indicated that, after the immobilization process on the surface of AEBSF-modified Uni-Graft prostheses, there were more structures with a high number of protrusions, while the introduction of modifications with a protein inhibitor led to the smoothing of their surface.

show abstract

Antimicrobial peptide coatings for hydroxyapatite: electrostatic and covalent attachment of antimicrobial peptides to surfaces

Cited by 48 publications

References 54 publications

Dextran-Thyme Magnesium-Doped Hydroxyapatite Composite Antimicrobial Coatings

Dextran-Thyme Magnesium-Doped Hydroxyapatite Composite Antimicrobial Coatings

Mechanism of Action of Surface Immobilized Antimicrobial Peptides Against Pseudomonas aeruginosa

Serine Protease Inhibitors—New Molecules for Modification of Polymeric Biomaterials

Contact Info

Product

Resources

About