Antimicrobial peptides from chilli pepper seeds causes yeast plasma membrane permeabilization and inhibits the acidification of the medium by yeast cells

Diz, Mariângela S.S.; Carvalho, André O.; Rodrigues, Rosana; Neves-Ferreira, Ana Gisele C.; Cunha, Maura Da; Alves, Elias W.; Okorokova‐Façanha, Anna L.; Oliveira, Marco Antônio de; Perales, Jonás; Machado, Olga Lima Tavares; Gomes, Valdirene Moreira

doi:10.1016/j.bbagen.2006.04.010

Cited by 83 publications

(58 citation statements)

References 35 publications

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“…a, c, e, g, i, k, m, o Cells viewed by DIC; b, d, f, h, j, l, n, p cells viewed by fluorescence Antonie van Leeuwenhoek (2012) 101:657-670 667 dose-dependent manner and with a pre-incubation time of 30 min. Diz et al (2006) demonstrated that the F1 fraction, isolated from Capsicum annuum seeds, at concentrations between 10 and 160 lg ml -1 , inhibited this acidification by 85-100%, suggesting that H ? -ATPases may be a potential target for the F1 fraction.…”

Section: Nitric Oxide Induction Determination Assaymentioning

confidence: 97%

“…During recent years, many plant proteins and peptides that can inhibit fungal development have been isolated and characterized. Examples of these compounds include 2S albumins (Agizzio et al 2003(Agizzio et al , 2006Ribeiro et al 2011), thionins (García-Olmedo et al 1998Stec 2006;Berrocal-Lobo et al 2009), defensins (Thevissen et al 2003;Games et al 2008;Mello et al 2011;Rogozhin et al 2011), lipid transfer proteins (LTPs) (Diz et al 2006;Carvalho and Gomes 2007;Zottich et al 2011) and proteinase inhibitors (PIs) (Terras et al 1993;Macedo et al 2007;Ribeiro et al 2007;Lopes et al 2009). …”

Section: Introductionmentioning

confidence: 97%

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Capsicum annuum L. trypsin inhibitor as a template scaffold for new drug development against pathogenic yeast

Ribeiro

Silva

Cunha

et al. 2011

Antonie van Leeuwenhoek

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A 6,000 Da peptide, named CaTI, was isolated from Capsicum annuum L. seeds and showed potent inhibitory activity against trypsin and chymotrypsin. The aim of this study was to determine the effect of CaTI on Saccharomyces cerevisiae, Candida albicans, Candida tropicalis and Kluyveromyces marxiannus cells. We observed that CaTI inhibited the growth of S. cerevisiae, K. marxiannus as well as C. albicans and induced cellular agglomeration and the release of cytoplasmic content. No effect on growth was observed in C. tropicalis but morphological changes were noted. In the spot assay, different degrees of sensitivity were shown among the strains and concentrations tested. Scanning electron microscopy showed that S. cerevisiae, K. marxiannus and C. albicans, in the presence of CaTI, exhibited morphological alterations, such as the formation of pseudohyphae, cellular aggregates and elongated forms. We also show that CaTI induces the generation of nitric oxide and interferes in a dose-dependent manner with glucose-stimulated acidification of the medium mediated by H(+)-ATPase of S. cerevisiae cells.

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Section: Nitric Oxide Induction Determination Assaymentioning

confidence: 97%

Section: Introductionmentioning

confidence: 97%

Capsicum annuum L. trypsin inhibitor as a template scaffold for new drug development against pathogenic yeast

Ribeiro

Silva

Cunha

et al. 2011

Antonie van Leeuwenhoek

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“…Plasma membrane permeabilization was measured by SYTOX green uptake as described previously by Diz et al (2006) with some modifications. Aliquots of 100 μL S. cerevisiae cells grown for 20 h in the presence of 200 μg/mL rEARLI1 were incubated with 0.2 μM SYTOX green for 30 min at 25°C with periodic agitation, followed by observation using an Olympus Fluoview FV1000 laser scanning confocal microscope equipped with a fluorescence filter set for fluorescein detection (excitation wavelengths, 450 to 490 nm; emission wavelength, 500 nm).…”

Section: Plasma Membrane Permeabilizationmentioning

confidence: 99%

Expression of recombinant EARLI1, a hybrid proline-rich protein of Arabidopsis, in Escherichia coli and its inhibition effect to the growth of fungal pathogens and Saccharomyces cerevisiae

Zhang

et al. 2012

Gene

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EARLI1 is an Arabidopsis gene with pleiotropic effects previously shown to have auxiliary functions in protecting plants against freezing-induced cellular damage and promoting germinability under low-temperature and salinity stresses. Here we determined whether recombinant EARLI1 protein has anti-fungal activity. Recombinant EARLI1 protein lacking its signal peptide was produced in Escherichia coli BL21(DE3) using isopropyl β-d-1-thiogalactopyranoside (IPTG) induction and the prokaryotic expression vector pET28a. Expression of EARLI1 was analyzed by Western blotting and the protein was purified using affinity chromatography. Recombinant EARLI1 protein was applied to fungal cultures of Saccharomyces cerevisiae, Botrytis cinerea and Fusarium oxysporum, and membrane permeability was determined using SYTOX green. Full-length EARLI1 was expressed in S. cerevisiae from the GAL1 promoter using 2% galactose and yeast cell viability was compared to control cells. Our results indicated that application of recombinant EARLI1 protein to B. cinerea and F. oxysporum could inhibit the growth of the necrotrophic fungi. Besides, addition of the recombinant protein to liquid cultures of S. cerevisiae significantly suppressed yeast growth and cell viability by increasing membrane permeability, and in vivo expression of the secreted form of EARLI1 in S. cerevisiae also had a remarkable inhibition effect on the growth of yeast cells.

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“…In an effort to combat diseases, plants have devised various mechanisms and compounds to fend off microbial invaders. One of the most ancient and widespread defense strategies involves the production of cysteine-rich antimicrobial peptides (AMPs) composed of 45 to 54 amino acids (13,14,20,37,57). AMPs have been shown to be produced by a wide range of organisms and serve as their natural defense against infection (60,61).…”

mentioning

confidence: 99%

Inhibition of Fungal and Bacterial Plant Pathogens In Vitro and In Planta with Ultrashort Cationic Lipopeptides

Makovitzki¹,

Viterbo

Brotman

et al. 2007

Appl Environ Microbiol

102

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Antimicrobial peptides from chilli pepper seeds causes yeast plasma membrane permeabilization and inhibits the acidification of the medium by yeast cells

Cited by 83 publications

References 35 publications

Capsicum annuum L. trypsin inhibitor as a template scaffold for new drug development against pathogenic yeast

Capsicum annuum L. trypsin inhibitor as a template scaffold for new drug development against pathogenic yeast

Expression of recombinant EARLI1, a hybrid proline-rich protein of Arabidopsis, in Escherichia coli and its inhibition effect to the growth of fungal pathogens and Saccharomyces cerevisiae

Inhibition of Fungal and Bacterial Plant Pathogens In Vitro and In Planta with Ultrashort Cationic Lipopeptides

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