In this study, porous microspheres of TiO 2 (µTiO 2) were synthesized, characterized and incorporated into an acrylic paint formulation to obtain a photocatalytically active paint. In a novel approach, the antifungal properties of the µTiO 2 paint were evaluated using Monascus ruber as the representative microorganism and compared to those of a photocatalyst-free paint. The photocatalytic activity of paint films was determined by methylene blue (MB) degradation under real conditions of application. High photocatalytic and antifungal activity was observed, with the microorganism culture showing the formation of growth inhibition halos, typical of materials that produce biocides that diffuse into the culture medium.