Antimicrobial poly(ionic liquid)-induced bacterial nanotube formation and drug-resistance spread

Mao, Haiyang; Guo, Jiangna; Zhou, Jinqiu; Shi, Jie; Cui, Hengqing; Shi, Rongwei; Yao, Jieyan; Fang, Xia; Wang, Bin; Yan, Feng

doi:10.1039/d2bm01130a

Cited by 8 publications

(4 citation statements)

References 40 publications

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“…To evaluate the PIL‐P‐Ce' role in preventing bacterial drug resistance spread, two E. coli strains with different ARGs and fluorescent genes were used for transformation experiments. [ 30 ] Plasmid solution from BL21 (carrying green GFP and Kan R genes) was incubated with the PIL‐P‐based membranes under 30 min of light, then mixed with DH5α (plasmid carrying red mCherry and Amp R genes) for transformation, followed by antibiotic screening, PCR, and fluorescence assays (Figure 3F ). Photos show that pristine DH5α can grow on the Amp plate, forming red colonies, while transformed bacteria could survive on the plate with both Amp and Kan, turning into white colonies after acquiring the BL21 strain's plasmid (Figure 3G ; Figure S12 , Supporting Information).…”

Section: Resultsmentioning

confidence: 99%

Antimicrobial and Antiviral Nanofibers Halt Co‐Infection Spread via Nuclease‐Mimicry and Photocatalysis

Yao,

Luo,

Lin

et al. 2024

Advanced Science

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The escalating spread of drug‐resistant bacteria and viruses is a grave concern for global health. Nucleic acids dominate the drug‐resistance and transmission of pathogenic microbes. Here, imidazolium‐type poly(ionic liquid)/porphyrin (PIL‐P) based electrospun nanofibrous membrane and its cerium (IV) ion complex (PIL‐P‐Ce) are developed. The obtained PIL‐P‐Ce membrane exhibits high and stable efficiency in eradicating various microorganisms (bacteria, fungi, and viruses) and decomposing microbial antibiotic resistance genes and viral nucleic acids under light. The nuclease‐mimetic and photocatalytic mechanisms of the PIL‐P‐Ce are elucidated. Co‐infection wound models in mice with methicillin‐resistant S. aureus and hepatitis B virus demonstrate that PIL‐P‐Ce integrate the triple effects of cationic polymer, photocatalysis, and nuclease‐mimetic activities. As revealed by proteomic analysis, PIL‐P‐Ce shows minimal phototoxicity to normal tissues. Hence, PIL‐P‐Ce has potential as a “green” wound dressing to curb the spread of drug‐resistant bacteria and viruses in clinical settings.

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Section: Resultsmentioning

confidence: 99%

Antimicrobial and Antiviral Nanofibers Halt Co‐Infection Spread via Nuclease‐Mimicry and Photocatalysis

Yao,

Luo,

Lin

et al. 2024

Advanced Science

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“…Another study reported that the antimicrobial activity of the imidazolium group is related to the presence of the hydroxyethyl chain and the methyl group in the imidazolium ring structure [ 48 ]. Conversely, the potential electrostatic interaction between the imidazolium cations and the negatively charged cell wall is the main antimicrobial force [ 49 , 50 ].…”

Section: Resultsmentioning

confidence: 99%

Bacterial Cellulose/Cellulose Imidazolium Bio-Hybrid Membranes for In Vitro and Antimicrobial Applications

Salama

Saleh

Cruz‐Maya

et al. 2023

JFB

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In biomedical applications, bacterial cellulose (BC) is widely used because of its cytocompatibility, high mechanical properties, and ultrafine nanofibrillar structure. However, biomedical use of neat BC is often limited due to its lack of antimicrobial properties. In the current article, we proposed a novel technique for preparing cationic BC hydrogel through in situ incorporation of cationic water-soluble cellulose derivative, cellulose bearing imidazolium tosylate function group (Cell-IMD), in the media used for BC preparation. Different concentrations of cationic cellulose derivative (2, 4, and 6%) were embedded into a highly inter-twined BC nanofibrillar network through the in situ biosynthesis until forming cationic cellulose gels. Cationic functionalization was deeply examined by the Fourier transform infrared (FT–IR), NMR spectroscopy, scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), and X-ray diffraction (XRD) methods. In vitro studies with L929 cells confirmed a good cytocompatibility of BC/cationic cellulose derivatives, and a significant increase in cell proliferation after 7 days, in the case of BC/Cell-IMD3 groups. Finally, antimicrobial assessment against Staphylococcus aureus, Streptococcus mutans, and Candida albicans was assessed, recording a good sensitivity in the case of the higher concentration of the cationic cellulose derivative. All the results suggest a promising use of cationic hybrid materials for biomedical and bio-sustainable applications (i.e., food packaging).

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“…In addition, K. pneumoniae HCD1 utilizes OMVs as a sophisticated tool for survival and resistance, potentially spreading resistance within bacterial communities. It harbors genes for three beta-lactamases, including the carbapenemase KPC-2, placing it among highly resistant strains [ 109 ]. In another case, Haemophilus influenzae ( H. influenzae ), produces β-lactamase and packages it into vesicles.…”

Section: Example Of a Bacterial Ev Responsible For Antibiotic Resistancementioning

confidence: 99%

Unseen Weapons: Bacterial Extracellular Vesicles and the Spread of Antibiotic Resistance in Aquatic Environments

Barathan,

Ng,

Lokanathan

et al. 2024

IJMS

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This paper sheds light on the alarming issue of antibiotic resistance (ABR) in aquatic environments, exploring its detrimental effects on ecosystems and public health. It examines the multifaceted role of antibiotic use in aquaculture, agricultural runoff, and industrial waste in fostering the development and dissemination of resistant bacteria. The intricate interplay between various environmental factors, horizontal gene transfer, and bacterial extracellular vesicles (BEVs) in accelerating the spread of ABR is comprehensively discussed. Various BEVs carrying resistance genes like blaCTX-M, tetA, floR, and sul/I, as well as their contribution to the dominance of multidrug-resistant bacteria, are highlighted. The potential of BEVs as both a threat and a tool in combating ABR is explored, with promising strategies like targeted antimicrobial delivery systems and probiotic-derived EVs holding significant promise. This paper underscores the urgency of understanding the intricate interplay between BEVs and ABR in aquatic environments. By unraveling these unseen weapons, we pave the way for developing effective strategies to mitigate the spread of ABR, advocating for a multidisciplinary approach that includes stringent regulations, enhanced wastewater treatment, and the adoption of sustainable practices in aquaculture.

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Antimicrobial poly(ionic liquid)-induced bacterial nanotube formation and drug-resistance spread

Abstract: Bacterial nanotubes are tubular membranous structures bulging from cell surface that can connect neighboring bacteria for the exchange of intercellular substances. However, little is known about the formation and function...

Cited by 8 publications

References 40 publications

Antimicrobial and Antiviral Nanofibers Halt Co‐Infection Spread via Nuclease‐Mimicry and Photocatalysis

Antimicrobial and Antiviral Nanofibers Halt Co‐Infection Spread via Nuclease‐Mimicry and Photocatalysis

Bacterial Cellulose/Cellulose Imidazolium Bio-Hybrid Membranes for In Vitro and Antimicrobial Applications

Unseen Weapons: Bacterial Extracellular Vesicles and the Spread of Antibiotic Resistance in Aquatic Environments

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