Antimicrobial potential and in vitro cytotoxicity study of polyvinyl pyrollidone‐stabilised silver nanoparticles synthesised from Lysinibacillus boronitolerans

Abstract

The main emphasis herein is on the eco‐friendly synthesis and assessment of the antimicrobial potential of silver nanoparticles (AgNPs) and a cytotoxicity study. Silver nanoparticles were synthesised by an extracellular method using bacterial supernatant. Biosynthesised silver nanoparticles were characterised by UV‐vis spectroscopy, transmission electron microscopy (TEM), Fourier transform infrared spectroscopy, dynamic light scattering, and zeta potential analysis. The synthesised silver nanoparticles exhibited a characteristic peak at 420 nm. TEM analysis depicted the spherical shape and approximately 20 nm size of nanoparticles. Silver nanoparticles carry a charge of −33.75 mV, which confirms their stability. Biogenic polyvinyl pyrrolidone‐coated AgNPs exhibited significant antimicrobial effects against all opportunistic pathogens (Gram‐positive and Gram‐negative bacteria, and fungi). Silver nanoparticles equally affect the growth of both Gram‐positive and Gram‐negative bacteria, with a maximum inhibition zone observed at 22 mm and a minimum at 13 mm against Pseudomonas aeruginosa and Fusarium graminearum , respectively. The minimum inhibitory concentration (MIC) of AgNPs against P. aeruginosa and Staphylococcus aureus was recorded at between 15 and 20 μg/ml. Synthesised nanoparticles exhibited a significant synergistic effect in combination with conventional antibiotics. Cytotoxicity estimates using C2C12 skeletal muscle cell line via 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) test and lactate dehydrogenase assay were directly related to the concentration of AgNPs and length of exposure. On the basis of the MTT test, the IC50 of AgNPs for the C2C12 cell line was approximately 5.45 μg/ml concentration after 4 h exposure.

Influence of Polyvinylpyrrolidone Concentration on Properties and Anti-Bacterial Activity of Green Synthesized Silver Nanoparticles

“…It has been shown that silver nanoparticles synthesized using an aqueous extract of Eucalyptus camaldulensis leaves and PVP as a stabilizing agent had no antibacterial properties because there was no zone of inhibition on the agar plates. This result conflicts with previous works that study the antibacterial properties of silver nanoparticles capped with PVP [ 36 , 50 ]. The absence of antibacterial activity is related to the formation of PVP layer around silver nanoparticles, which prevented the release of silver ions.…”

“…This study highlights the absence of any antibacterial activity for silver nanoparticles prepared using PVP. To the best of our knowledge, there is no similar results have been reported earlier in the literatures [ 36 , 37 ]. This result support the benefit of coated silver nanoparticles with PVP to control the release ratio of silver ions and reduce the toxicity effect of AgNPs against living cells especially for medical and pharmacology applications as drug delivery systems and wound healing without any side effects [ 38 ].…”

Monitoring In Vitro Extracellular Matrix Protein Conformations in the Presence of Biomimetic Bone-Regeneration Scaffolds Using Functionalized Gold-Edge-Coated Triangular Silver Nanoparticles

“…When in presence of a CS-HAp scaffold, active Fn showed similar red-shifting performance ( Figure 4 a) with shifts as long as 80 nm between the 8 h and 32 h time points ( Table 2 ).The morphology of C2C12 cells was observed to be normal with a high percentage of confluence, which indicated that the NPs had no observable negative impact on the cell culture regardless of the treatment up to 32 h ( Figure 4 b). This result is in keeping with findings in the literature, which reports that C2C12 cells exposed to low concentrations of silver nanoparticles show no negative effect on morphology or cytotoxicity between 0.25 and 2 ug/mL [ 29 ].…”

Unveiling the cytotoxic and anti-proliferative potential of green-synthesized silver nanoparticles mediated byColletotrichum gloeosporioides

“… 85 The biogenically synthesized AgNPs from L. boronitolerans has shown significant cytotoxic action against C2C12 muscle cell line that leads to distortion of cellular membrane in a concentration dependent manner. 86 Therefore, the result validates that green synthesized nanoparticles CgAgNPs can serve as a potential cytotoxic as well as anti-proliferative agent against the breast cancer cells.…”