Antimicrobial potential of alkalophilic micromycetes Emericellopsis alkalina

Baranova, Anna A.; Георгиева, М. Л.; Биланенко, Е. Н.; Andreev, Yaroslav A.; Рогожин, Е. А.; Садыкова, В. С.

doi:10.1134/s0003683817060035

Cited by 18 publications

(19 citation statements)

References 20 publications

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“…Compounds that are hydrophobic to different degrees (based on the retention time in separation by liquid chromatography) were found in the elution zone of the main component, emericellipsin A. Previously, it was shown that, among the entire spectrum of metabolites of E. alkalina A118, two compounds in addition to substance A118/37 (emericellipsin A) possessed similar functional activity: A118/35 (B) and A118/36 (C) [ 19 ]. These two compounds were eluted from the column with lower retention times (61.4 and 62.9 min, respectively) and displayed strong antimicrobial activity which was close to that of emericellipsin A.…”

Section: Resultsmentioning

confidence: 99%

“…Previously, the 65 strains of E. alkalina were isolated from soils near soda lakes in Siberia, Trans-Baikal regions (Russia), and Eastern Mongolia. Primary screening among isolates of this species revealed in E. alkalina strain VKPM F1428 the presence of antimicrobial activity against pathogenic micromycetes [ 19 , 20 ]. Furthermore, researchers carried out the isolation and structural and functional characterization of a new, previously undescribed, secreted antimicrobial peptide called emericellipsin A (EmiA), which is a product of nonribosomal synthesis and belongs to the group of peptaiboles [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

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The Emericellipsins A–E from an Alkalophilic Fungus Emericellopsis alkalina Show Potent Activity against Multidrug-Resistant Pathogenic Fungi

Куварина¹,

Gavryushina²,

Kul'ko

et al. 2021

JoF

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Novel antimicrobial peptides with antifungal and cytotoxic activity were derived from the alkalophilic fungus Emericellopsis alkalina VKPM F1428. We previously reported that this strain produced emericellipsin A (EmiA), which has strong antifungal and cytotoxic properties. Further analyses of the metabolites obtained under a special alkaline medium resulted in the isolation of four new homologous (Emi B–E). In this work, we report the complete primary structure and detailed biological activity for the newly synthesized nonribosomal antimicrobial peptides called emericellipsins B–E. The inhibitory activity of themajor compound, EmiA, against drug-resistant pathogenic fungi was similar to that of amphotericin B (AmpB). At the same time, EmiA had no hemolytic activity towards human erythrocytes. In addition, EmiA demonstrated low cytotoxic activity towards the normal HPF line, but possessed cancer selectivity to the K-562 and HCT-116 cell lines. Emericillipsins from the alkalophilic fungus Emericellopsis alkaline are promising treatment alternatives to licensed antifungal drugs for invasive mycosis therapy, especially for multidrug-resistant aspergillosis and cryptococcosis.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Emericellipsins A–E from an Alkalophilic Fungus Emericellopsis alkalina Show Potent Activity against Multidrug-Resistant Pathogenic Fungi

Куварина¹,

Gavryushina²,

Kul'ko

et al. 2021

JoF

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“…Emericellipsin A was isolated from fungal culture liquid as described previously with modification [ 7 ]. The scheme includes a combination of ethyl acetate extraction followed by evaporation, dissolving in ethanol and analytical reversed-phase HPLC on a C 18 phase [ 7 ]. One additional purification step, based on analytical phenyl RP-HPLC, was used to obtain the individual component.…”

Section: Resultsmentioning

confidence: 99%

“…Isolation of the target peptaibol from the culture liquid was carried accordingly described earlier [ 7 ]. Rechromatography of the emericellipsin A-containing fraction was performed on a Synergi Polar-RP (250 × 4.6 mm 4 µm 80 Å) analytical column (Phenomenex, Torrance, CA, USA) in a linear gradient of acetonitrile/isopropanol (4:1, w / w ) mixture with 0.1% trifluoriacetic acid (TFA) from 16 to 85% for 45 min, flow rate of 1 mL/min and detection of absorbance at 210 nm.…”

Section: Methodsmentioning

confidence: 99%

“…Peptaibols isolated from Emericellopsis species include zervamicins (produced by E. microspora ) [ 1 , 2 ], bergofungins A and B (produced by E. donezkii ), bergofungins C and D (produced by E. salmosynnemata ) [ 3 , 4 , 5 ], and heptaibin and emerimicines (produced by E. minima ) [ 6 ]. Screening of metabolites of several alkalophilic strains isolated from saline soils has revealed the fungus Emericellopsis alkalina strain VKPM F-1428, which demonstrates promising antifungal activity against different fungal taxons [ 7 ]. Bioassay-guided fractionation makes it possible to isolate the novel peptaibol, termed emericellipsin A.…”

Section: Introductionmentioning

confidence: 99%

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A Novel Lipopeptaibol Emericellipsin A with Antimicrobial and Antitumor Activity Produced by the Extremophilic Fungus Emericellopsis alkalina

et al. 2018

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Soil fungi are known to contain a rich variety of defense metabolites that allow them to compete with other organisms (fungi, bacteria, nematodes, and insects) and help them occupy more preferential areas at the expense of effective antagonism. These compounds possess antibiotic activity towards a wide range of other microbes, particularly fungi that belong to different taxonomical units. These compounds include peptaibols, which are non-ribosomal synthesized polypeptides containing non-standard amino acid residues (alpha-aminoisobutyric acid mandatory) and some posttranslational modifications. We isolated a novel antibiotic peptide from the culture medium of Emericellopsis alkalina, an alkalophilic strain. This peptide, called emericellipsin A, exhibited a strong antifungal effect against the yeast Candida albicans, the mold fungus Aspergillus niger, and human pathogen clinical isolates. It also exhibited antimicrobial activity against some Gram-positive and Gram-negative bacteria. Additionally, emericellipsin A showed a significant cytotoxic effect and was highly active against Hep G2 and HeLa tumor cell lines. We used NMR spectroscopy to reveal that this peptaibol is nine amino acid residues long and contains non-standard amino acids. The mode of molecular action of emericellipsin A is most likely associated with its effects on the membranes of cells. Emericellipsin A is rather short peptaibol and could be useful for the development of antifungal, antibacterial, or anti-tumor remedies.

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Microbial communities in paddy soil as influenced by nitrogen fertilization and water regimes

Sun

Wang

et al. 2021

Agronomy Journal

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Plastic film mulching (PM) cultivation is an important measure to alleviate the seasonal shortage of water resources. Long‐term nitrogen (N) fertilization under PM cultivation may be more conducive to enhance the ecological relationship between microbial communities involved in nutrient cycling. In this study, the effects of long‐term application of chemical N fertilizer (common prilled urea [PU]) or no N fertilization on the microbial community composition under the traditional flooding (TF) cultivation and PM cultivation were comparatively analyzed. The results demonstrated that water regimes had significant effects on all soil properties except alkali‐hydrolyzable N. Compared with TF cultivation, PM cultivation increased pH and available phosphorus (AP) by 1.14 and 13.67%, respectively, and decreased organic matter and available potassium (AK) by 6.07 and 8.39%, respectively. Nitrogen fertilization levels had significant effects on soil AP and AK, with increases of 1.39 and 1.82 mg kg−1 in PU treatment compared with CK, respectively. Neither water regime nor N fertilization level had a significant influence on soil microbial richness (Chao index) and diversity (Shannon index). However, water regimes played critical roles in driving microbial community changes. Network analysis showed that the microbial community had nonrandom co‐occurrence pattern. Myrmecridium, Defluviicoccus, Emericellopsis, Pseudarthrobacter, and Syntrophorhabdus were the keystone taxa. A higher relative abundance of Defluviicoccus, Emericellopsis, and Syntrophorhabdus was observed in the CKPM treatment, and a higher relative abundance of Myrmecridium and Pseudarthrobacter was observed in the PUPM treatment. Therefore, PM cultivation is an effective measure to improve potential beneficial taxa of soil microbial communities.

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Antimicrobial potential of alkalophilic micromycetes Emericellopsis alkalina

Cited by 18 publications

References 20 publications

The Emericellipsins A–E from an Alkalophilic Fungus Emericellopsis alkalina Show Potent Activity against Multidrug-Resistant Pathogenic Fungi

The Emericellipsins A–E from an Alkalophilic Fungus Emericellopsis alkalina Show Potent Activity against Multidrug-Resistant Pathogenic Fungi

A Novel Lipopeptaibol Emericellipsin A with Antimicrobial and Antitumor Activity Produced by the Extremophilic Fungus Emericellopsis alkalina

Microbial communities in paddy soil as influenced by nitrogen fertilization and water regimes

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