Antimicrobial potential of phylogenetically unique actinomycete, Streptomyces sp. JRG-04 from marine origin

Govindarajan, Ganesan; Santhi, Velayudhan Satheeja; Jebakumar, Solomon Robinson David

doi:10.1016/j.biologicals.2014.08.003

Cited by 24 publications

(12 citation statements)

References 37 publications

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“…This un-trapped microbial diversity of the mangrove sediments is a potential resource for exploring novel bioactive compounds. India has many such unexplored mangrove regions with rich source of novel metabolites (Ganesan et al 2014 ). In view of the significance of mangrove ecosystem the present study is aimed to evaluate bio prospective study of bioactive compounds isolated from actinomycetes of mangrove sediment samples together with the extraction, isolation, and structure elucidation of bioactive compounds.…”

Section: Introductionmentioning

confidence: 99%

Bioactive metabolites produced by Streptomyces Cheonanensis VUK-A from Coringa mangrove sediments: isolation, structure elucidation and bioactivity

et al. 2016

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The strain VUK-A was isolated from a sediment sample of the Coringa mangrove ecosystem was identified as Streptomyces cheonanensis based on morphological, physiological, biochemical and molecular properties. Chemical investigation of the secondary metabolites of the strain Streptomyces cheonanensis VUK-A has led to the segregation of two bioactive compounds, namely 2-Methyl butyl propyl phthalate (1) and Diethyl phthalate (2) using column chromatography. The chemical structure of the active compounds was established on the basis of spectroscopic analysis, including 1H NMR and 13C NMR spectroscopies, FTIR and EIMS. The antimicrobial activity of the bioactive compounds produced by the strain was tested against a wide variety of bacteria and fungi and expressed in terms of minimum inhibitory concentration. The compounds (1&2) were active against all the bacteria tested, and the best activity of compound 1 was recorded against Proteus vulgaris (4 µg/ml). Compounds (1&2) were active against dermatophytes and fungi but compound 1 displayed high antifungal activity against Candida albicans (8 µg/ml) and Fusarium solani (16 µg/ml) compared to standard antifungal agents. The cytotoxicity of the bioactive compound 1 was tested against MDA-MB-231, OAW-42, HeLa, and MCF-7 cell lines. The highest activity of 100 µM by compound 1 was recorded against HeLa cancer cell lines. In fact, this is the first report of 2-Methyl butyl propyl phthalate from the genus Streptomyces.Electronic supplementary materialThe online version of this article (doi:10.1007/s13205-016-0398-6) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Bioactive metabolites produced by Streptomyces Cheonanensis VUK-A from Coringa mangrove sediments: isolation, structure elucidation and bioactivity

et al. 2016

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“…The broad-spectrum bioactive property and non-cytotoxicity against normal H9C2 cell lines are due to the aromatic polyketide nature of the compound. This feature is an important criterion for developing antimicrobials against pathogens [7]. However, its bioactive production potential titer is significantly affected by incubation conditions and components of the unoptimized production medium.…”

Section: Discussionmentioning

confidence: 99%

“…Many recent studies have reported the occurrence of novel Streptomyces species in marine environments rich in diverse chemicals used for the development of therapeutic materials [6]. During characterization of novel marine Streptomyces with a potential for bioactive compound production, we recently reported [7] a marine Streptomyces sp. JRG-04, which displayed bioactivity against various disease-causing infectious microbial pathogens, including MDR clinical pathogens, such as methicillin-resistant Staphylococcus aureus and vancomycin-resistant enterococci.…”

Section: Introductionmentioning

confidence: 99%

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Statistical optimization of medium constituents and conditions for improved antimicrobial compound production by marine Streptomyces sp. JRG-04

Govindarajan¹,

Santhi²,

David³

2017

ARCH BIOL SCI BELGRA

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A recently isolated Streptomyces sp. JRG-04 from a mangrove estuary was identified as a producer of a broadspectrum antimicrobial compound against various pathogens, including multidrug resistant (MDR) pathogens, with no cytotoxic effect on H9C2 cells. In this study, the concentrations of various nutrient factors and culture conditions were optimized by both classical and statistical methods for an improved titer of the antimicrobial compound production. Among nutrient factors, carbon and nitrogen sources such as maltose and yeast extract stimulated the production of the antimicrobial compound with the highest titer. The production medium, with a pH 7.5 at 28°C, promoted increased antimicrobial compound production. All non-statistically optimized nutrients and environmental conditions were used for subsequent statistical optimization using a Plackett-Burman design (PBD) and response surface methodology (RSM). Maltose, yeast extract and the inorganic salt NH 4 Cl were found to be significant components for antimicrobial compound production by the PBD method. Interactions between important variables were evaluated using central composite design (CCD) of response surface methodology. The final optimized medium (L -1 ) contained: 10 g maltose, 2.9 g Na 2 HPO 4 , 2.3 g KH 2 PO 4 , 1 g NH 4 Cl, 0.5 g MgSO 4 ×7H 2 O, 0.002 g FeSO 4 , 0.5 g CaCO 3 , 5.25 g yeast extract and trace elements in 5.0 mL salt solution (0.1 g ZnSO 4 ×7H 2 O, 0.3 g H 3 BO 3 , 0.2 g COCl 2 ×6H 2 O, 0.03 g MnCl 2 4H 2 O, 0.03 g Na 2 MO 4 ×2H 2 O, 0.02 g NiCl 2 ×6H 2 O, 0.01 g CuCl 2 ×2H 2 O). The medium provided an overall 42.8% increase in antibiotic activity when compared to the unoptimized medium, from 140.57±0.80 to 210.33±0.57 U/mL.

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“…Existing antibiotics cannot deal with this situation. In order to overcome these problems, the exploration of new and potent antibiotic agents is urgently needed [1]. Actinomycetes are the most critical of all microorganisms for the production of bioactive metabolites with potential applications in human health care [2,3].…”

Section: Introductionmentioning

confidence: 99%

Application of response surface methodology to optimize the production of antimicrobial metabolites by Micromonospora Y15

Wang

Zhang

et al. 2017

Biotechnology & Biotechnological Equipment

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The fermentation medium of Micromonospora Y15 was optimized to enhance the production of antimicrobial metabolites with the antimicrobial activity tested against Listeria monocytogenes. First, the optimal medium components (carbon source, nitrogen source and inorganic salt) were determined by the single factor experiment. Second, the Plackett-Burman experiment was used to screen significant factors. Third, the steepest ascent experiment was undertaken to figure out the optimal region of the significant factors. Finally, the central composite experiment was conducted to optimize the final medium components. The single factor experiment results suggested that the optimal medium components were soluble starch, beef extract, peptone, NaCl, K 2 HPO 4 , MgSO 4 , and FeSO 4 , while the Plackett-Burman experiment demonstrated that beef extract, K 2 HPO 4 and soluble starch were significant factors affecting antimicrobial activity. The steepest ascent experiment results further showed the central point of three significant factors were 1.00 g/L of K 2 HPO 4 , 25.00 g/L of soluble starch and 18.00 g/L of beef extract, respectively. Response surface analysis revealed that the optimum values of the tested significant variables for the production of antimicrobial metabolites were 1.03 g/L of K 2 HPO 4 , 27.67 g/L of soluble starch and 18.30 g/L of beef extract, accordingly. Under this optimal condition, the antimicrobial activity of the fermented medium was 1236.79 § 14.56 AU/mL, which increased 3.86 times compared with the initial medium. We thus concluded that the medium composition optimized in the study would be helpful for the production of antimicrobial metabolites by Micromonospora Y15.

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Antimicrobial potential of phylogenetically unique actinomycete, Streptomyces sp. JRG-04 from marine origin

Cited by 24 publications

References 37 publications

Bioactive metabolites produced by Streptomyces Cheonanensis VUK-A from Coringa mangrove sediments: isolation, structure elucidation and bioactivity

Bioactive metabolites produced by Streptomyces Cheonanensis VUK-A from Coringa mangrove sediments: isolation, structure elucidation and bioactivity

Statistical optimization of medium constituents and conditions for improved antimicrobial compound production by marine Streptomyces sp. JRG-04

Application of response surface methodology to optimize the production of antimicrobial metabolites by Micromonospora Y15

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