Antimicrobial potential of TiO₂nanoparticles against MDRPseudomonas aeruginosa

Abstract

The present study aims to evaluate the inhibition effectiveness of titanium dioxide nanoparticles in combination with cell wall active antibiotics -ceftazidime and cefotaxime against the multi-drug resistant Pseudomonas aeroginosa isolated from pus, sputum, endo-tracheal tract and broncho-alveolar lavage. Commercial Degussa-P25 TiO 2 nanoparticle, antibiotics ceftazidime and cefotaxime were used in this study against multi-drug-resistant nosocomial pathogen. The nanoparticle shows antimicrobial effect on the pathogen at concentrations more than 350 mg/mL, when exposed to ultraviolet (UV) light for an hour. Minimum inhibitory concentration values obtained for the antibiotic cefotaxime were sixfolds higher than the antibiotic ceftazidime. When these antibiotics were used in combination with UV-irradiated metal nanoparticle, ceftazidime resulted in enhanced antimicrobial activity whereas cefotaxime does not show any change.

Nanomaterials as Delivery Vehicles and Components of New Strategies to Combat Bacterial Infections: Advantages and Limitations

“…For instance, Maneerat et al [77] implemented its antifungal activity against Penicillium expansum to test post-harvest disease control. The potential of TiO 2 NPs against MDR P. aeruginosa was evaluated by Arora et al [78]. Exposure of TiO 2 NPs to UV irradiation seems to enhance the antibacterial activity.…”

Improving the corrosion resistance of micro-arc oxidation coated Mg–Zn–Ca alloy

“…The TiO 2 phase possessed catalytic activity and highly antibacterial properties. [50][51][52] Furthermore, the free radicals on TiO 2 can induce the formation of apatite that subsequently promoted the adhesion of osteoblast. 53,54 The formation of calcium salts indicated that the incorporation of n-HAp occurred by reactive way during MAO process.…”

<p>Comparative Study of Antibacterial Effects of Titanium Dioxide Nanoparticles Alone and in Combination with Antibiotics on MDR <em>Pseudomonas aeruginosa</em> Strains</p>

“…55 Many studies have shown that combining TDN potentiate the antimicrobial action of different classes of antibiotics. 2,[7][8][9] FICI is an indicator of the degree of interaction between TDN with commercial antibiotics for the 25 MDR P. aeruginosa strains. The FICI of the combination of TDN plus cefepime shows synergistic effect to all isolates (100%), ceftriaxone; showing synergistic effect against 24 strains (96%) and additive effect against one strain (4%); ciprofloxacin showing synergistic effect against 20 strains (80%) and additive effect against three (12%) and two strains were not evaluated using the combination of TDN and ciprofloxacin as both were sensitive to ciprofloxacin; amikacin, showing synergistic effect against 22 strains (88%) and additive effect against three (12%) isolates as shown in Table 3.…”

“…Pseudomonas aeruginosa is an opportunistic pathogen that causes a variety of diseases ranging from mild to severe life-threatening infections and considered as one of the main causes of nosocomial or hospital-acquired infections. [1][2][3] P. aeruginosa infections are difficult to eradicate by many antimicrobial agents due to its intrinsic resistance (low outer membrane permeability, efflux mechanisms, and inactivity of the antibiotics by enzymes), acquired resistance (mutations in genes targeted by the antibiotics), adaptive resistance (biofilm formation), 4 and various virulence factors (extracellular proteases, toxins). 5 Treatment of these infections has become a great challenge because of the ability of this bacterium to resist a variety of antibiotics, including aminoglycosides, quinolones, and β-lactams.…”