ANTIMICROBIAL PROPERTIES OF THE ESSENTIAL OIL OF <i>JUNIPERUS COMMUNIS</i> (L.) GROWING WILD IN EAST PART OF KOSOVO

Abstract

Antimicrobial activity and chemical composition of the essential oil of Juniperus communis (L.), originated from east part of Kosova, was investigated. The essential oil from berries of J. communis (L.), obtained by hydro-distillation was analyzed by GC and GC-MS. Antimicrobial properties of the essential oil of J. communis (L.) are investigated and results are submitted for their activities against Staphylococcus aureus, Escherichia coli, Hafnia alvei and Pseudomonas aeruginosa. Applying the agar disc diffusion technique, we measured diameters of the inhibition zone around discs, which are previously wetted with DMF solution of the essential oil with three different concentrations, 1, 3 and 5 mg mL −1 . Analysis of the oil resulted in the identification of 41 peaks, representing 96% of the oil. Berry essential oil composed mainly of monoterpenoids which amounted to 83%, of which 69.4% was monoterpene hydrocarbons. The main monoterpene hydrocarbons were α-pinene (36.2%) and β-myrcene (21.1%). The sesquiterpene accounted for about 13.4% of the total oil composition. Germacrene D (2.2%), α-cadinol (1.6%), α-humulene (1.5%), spathulenol (1.4%), epi-α-bisabolol (1.3%) and germacrene B (1.1%) were the main constituents of the sesquiterpenes. The inhibition zone depends from concentrations and also from sort of bacteria. The inhibition zones differ from 0-39 mm. The present work presents the chemical composition of the hydrodistilled oil of J. communis (L.) from East part of Kosova and the results are compared to those reported in the literature. This study demonstrates the occurrence of α-pinene chemotype of J. communis (L.) from east part of Kosova. The essential oil of J. communis (L.) growing wild in Kosova, showed moderate to high activities against Staphylococcus aureus, Escherichia coli, Hafnia alvei. Pseudomonas aeruginosa is resistant to the essential oil of J. communis (L.) growing wild in east part of Kosova.

Chemical composition and antimicrobial activity of hydrodistilled oil from juniper berries

“…On the other hand, E. aerogenes, P. aeruginosa and S. typhimurium were the least susceptible, with only one EO being active against these bacteria at the tested concentrations. This is in good agreement with previous studies on the antimicrobial activity of juniper berries EO (Filipowicz et al, 2003;Haziri et al, 2013), as well as with data available in the literature that describe Gram-positive bacteria has being more susceptible to essential oils than Gram-negative bacteria (Burt, 2004;Silva et al, 2013), possibly due to the presence of lipoproteins and lipopolysaccharides in Gram-negative bacteria that form a barrier to hydrophobic compounds (Mann et al, 2000).…”

“…One of the most marked differences concerns the content of β-pinene and sabinene, which were very high and very low, respectively, when compared with previous data (Angioni et al, 2003;Foudil-Cherif and Yassaa, 2012;Glišić et al, 2007;Matović et al, 2011). However, considering only the qualitative profile, the Portuguese berries presented some similarities with juniper berries from Kosovo, in terms of their major components (Haziri et al, 2013). By the contrary, the chemical composition was very different from the one previously reported for juniper berries collected in the central region of Portugal (Cavaleiro et al, 2006) that presented β-phellandrene and α-terpinyl acetate among the major compounds, those being absent in the herein evaluated sample.…”

Chemical composition and antimicrobial activity of leaves essential oil of Juniperus communis (Cupressaceae) grown in Republic of Macedonia

“…GC/MS analysis of the essential oil showed that predominant constituents in this oil were α-pinene (29.17%), β-pinene (17.84%), sabinene (13.55%), limonene (5.52%) and β-myrcene (0.33%) (Pepelnjak et al, 2005). The essential oil of J. communis growing wild in Kosovo, showed moderate to high activities against Staphylococcus aureus, Escherichia coli and Hafnia alvei, while Pseudomonas aeruginosa was resistant to the antimicrobial effects of the oil (Haziri et al, 2013). The leaves essential oils of J. communis subsp.…”

Phytochemicals and Antioxidant Study of Teucrium Chamaedrys (L.) Plant

“…Our research group was interested to analyse the chemical profile of different medicinal plants, which are growing wild in the region of Kosovo and Albania (Faiku et al, 2012(Faiku et al, , 2015a(Faiku et al, ,b, 2016(Faiku et al, ,b, 2018Haziri et al, 2009Haziri et al, , 2010Haziri et al, , 2013Haziri et al, , 2017a.…”