Antimicrobial property and antiproliferative activity of Centaurea babylonica (L.) L. on human carcinomas and cervical cancer cell lines

Abstract

Introduction and objective. Since antiquity, C. babylonica (L.) L. extracts has been used as a remedy for primary health care in traditional medicine. In this study, a total of seven different crude extracts (acetone, chloroform, hexane, ethylacetate, methanol, ethanol and water) from branches and leaves of C. babylonica (L.) L. were prepared to determine antimicrobial and antiproliferative activity against cancer cell lines. Materials and method. MIC assay was used for antimicrobial activity against gram positive and gram negative bacteria, and one yeast. MTT assay was applied to screen the antiproliferative activity of seven extracts, and to determine dose-and time-dependent effects of the aceton extract on A549, PC-3, MCF-7, and HeLa cell lines. Results. The aceton extract of C.babylonica (L.) L. showed the best antibacterial activity against Bacillus cereus, P. aeruginosa and C. albicans (MIC: 1.6 mg/mL). GC-MS analyses allowed six compounds to be determined; the main constituents of acetone extract from C. babylonica (L.) L. were diacetone alcohol (53.47 %), 1-dexadecene (10.19 %) and 1-tetradecene (8.67 %). In addition, seven different solvent extracts at 500 µg/mL caused antiproliferative activity between 84%-88%, compared to control. Dose-dependent effects of the extracts on A549 cells indicated that chloroform, ethyl acetate, and aceton extract were the most effective extracts with the IC 50 values of 9, 33, and 36 µg/mL, respectively. Conclusions. The results clearly demonstrate that C. babylonica (L.) L. exhibited a strong antimicrobial effect and antiproliferative activity against cancer cells in vitro. Further studies are required to isolate and characterize the active pure compounds responsible for the antimicrobial and antiproliferative activities.

Comparative Assessment of the Phytochemical Composition and Biological Activity of Extracts of Flowering Plants of Centaurea cyanus L., Centaurea jacea L. and Centaurea scabiosa L.

“…In the vast majority of cases, species native to Turkey (e.g., Centaurea antiochia var., Centaurea hypoleuca , Centaurea amaena Boiss. & Balansa, Centaurea aksoyi Hamzaoglu & Budak, Centaurea babylonica L.) [ 13 , 14 , 15 ] and Africa ( Centaurea pumilio L. [ 28 ] and 26 other species [ 4 ]) are studied.…”

“…The data in the literature suggest that antimicrobial activity in Centaurea species varies greatly from low (MIC > 20 mg/mL [ 9 , 14 , 15 , 28 ]) to high (MIC < 20 μg/mL) [ 13 , 34 , 35 ].…”

“…The most characteristic biologically active components are sesquiterpene lactones [ 11 , 12 ], flavonoids, lignans, alkaloids [ 9 ], phenolic compounds [ 13 , 14 ], steroids, and terpenes, etc. [ 4 , 15 , 16 ].…”

Phytochemical Composition,In VitroAntimicrobial, Antioxidant, and Enzyme Inhibition Activities, andIn SilicoMolecular Docking and Dynamics Simulations ofCentaurea lycaonica: A Computational and Experimental Approach

“… 48 The acetone extract of C. babylonica showed the best antibacterial activity against Bacillus cereus , P. aeruginosa , and C. albicans (MIC: 1.6 mg/mL). 49 A study determined the antioxidant capacities of C. balsamita and C. albonitens seed extracts by DPPH and ABTS assays. Results were 26.60 and 27.12% in DPPH and 80.61 and 95.99 mmol Trolox eq/g in ABTS, respectively.…”

The antimicrobial and antioxidant property, GC–MS analysis of non-edible oil-seed cakes of neem, madhuca, and simarouba

“…The hot neem extract contained previously known antioxidants that includes methyl gallate (Lubis et al 2018), vinyl sulphide (Ianiski et al 2018), 5, hydroxymethylfurfural (Zhao et al 2013), 6-hydroxyl-3-phenyl coumarin (Matos et al 2015), cyclic octa atomic sulfur (Ojinnaka et al 2015). The anti-HIV metabolites include methyl gallate (0.39%), canthin-6-one (2.02%) from neem hot and simarouba cold acetone extracts, respectively (Guvensen et al 2019;Wang et al 2014;Ahmed et al 2017), while 2,4-di-tert-butylphenol and vanillic acid are also anticancer agents besides its antimicrobial, antioxidants activity (Gazoni et al 2018;Yemis et al 2011;Dehpour et al 2012). On the other hand, angelic acid is identified as the potent antioxidants, spasmolytic, besides its usefulness to treat nervous problems (Varsha et al 2015).…”