No abstract

Introduction and objective. Since antiquity, C. babylonica (L.) L. extracts has been used as a remedy for primary health care in traditional medicine. In this study, a total of seven different crude extracts (acetone, chloroform, hexane, ethylacetate, methanol, ethanol and water) from branches and leaves of C. babylonica (L.) L. were prepared to determine antimicrobial and antiproliferative activity against cancer cell lines. Materials and method. MIC assay was used for antimicrobial activity against gram positive and gram negative bacteria, and one yeast. MTT assay was applied to screen the antiproliferative activity of seven extracts, and to determine dose-and time-dependent effects of the aceton extract on A549, PC-3, MCF-7, and HeLa cell lines. Results. The aceton extract of C.babylonica (L.) L. showed the best antibacterial activity against Bacillus cereus, P. aeruginosa and C. albicans (MIC: 1.6 mg/mL). GC-MS analyses allowed six compounds to be determined; the main constituents of acetone extract from C. babylonica (L.) L. were diacetone alcohol (53.47 %), 1-dexadecene (10.19 %) and 1-tetradecene (8.67 %). In addition, seven different solvent extracts at 500 µg/mL caused antiproliferative activity between 84%-88%, compared to control. Dose-dependent effects of the extracts on A549 cells indicated that chloroform, ethyl acetate, and aceton extract were the most effective extracts with the IC 50 values of 9, 33, and 36 µg/mL, respectively. Conclusions. The results clearly demonstrate that C. babylonica (L.) L. exhibited a strong antimicrobial effect and antiproliferative activity against cancer cells in vitro. Further studies are required to isolate and characterize the active pure compounds responsible for the antimicrobial and antiproliferative activities.

The ethanol, methanol, hexane, chloroform, isopropanol and water extract of leaves were tested for antimicrobial activity against eleven bacteria and one yeast by disc diffusion method. The antimicrobial activity was assessed and the volatile components of chloroform and dimethyl sulfoxide extracts of leaves were analysed by GC/MS. In the present study, chloroform extracts of leaves showed the best inhibition zone (27 mm) against Inhibition zones of the all the extracts varied from to 27 mm against tested microorganisms. The minimum inhibitory concentration (MIC) of subsp. extracts obtained by broth serial dilution method, chloroform extracts were among the most active with the MIC values ranging from 0.016-128 mg ml. showed lowest sensitivity to 0.016 mg ml concentration of chloroform extracts. GC/MS analyses of dimethyl sulfoxide extracts identified three compounds; the primary content of lemon balm leaves extract w dimethyl sulfoxide (98.53%). Similarly, GC/MS analyses of chloroform extract identified eight compounds; the primary contents of the of lemon balm leaves extract were chloroform (96.6%). Different solvent extracts of leaves showed a broad-spectrum activity against both Gram-positive and Gram-negative bacteria and fungi. Chloroform and dimethyl sulfoxide extracts, especially showed better antimicrobial activity due to high content of chloroform and dimethyl sulfoxide.

As a result, biofilm formation in cage bird feces originated ESBL positive Escherichia coli isolates was performed for the first time in Turkey. In order to present the relation between pathogenicity and biofilm production in animal originated ESBL positive isolates, further studies are required.

Different extracts of Liquidambar orientalisleaves were tested for their antimicrobial activity against eleven bacterial and one yeast strain by disc diffusion method. The antimicrobial activity was measured by disc diffusion and MIC. After that among extracts the best antimicrobial activity was detected.Volatile components of the ethanolic extracts of leaves of sweetgum analysed by GC/MS. Ethanolic extracts of sweetgum leaves showed the best inhibition zones against Klebsiella pneumoniae (32mm). K.pneumoniae CCM 2318 showed the lowest sensitivity to 0.008 mg/mL concentration of ethanolic extracts. Ethanolic extracts of L. orientalis var. orientalis were showed the best antimicrobial activity MIC values of ethanolic extracts ranging from 0.008-64 mg.mL-1 to the tested bacteria. The GC/MS analyses allowed seven compounds to be determined; benzenepropanol (%49.30) and cinnamic acid (%35.89) were the main constituents of the of sweetgum leaves extract. All the extracts of L. orientalis leaves showed varying degrees of antimicrobial activity on the microorganisms tested. The antimicrobial activity was due to the presence the essential oils. Among the tested extracts the ethanolic extracts were the most active against the microorganisms tested compared to the reference antibiotics.