Effects of magnesium and calcium cations on biofilm formation by Sphingomonas paucimobilis from an industrial environment

Güvensen, Nur Ceyhan; Demir, Serdar; Özdemir, Güven

doi:10.1016/j.copbio.2013.05.185

Cited by 17 publications

(19 citation statements)

References 37 publications

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“…Calcium is thought to promote thicker bacterial biofilms, primarily through ionic bridging of the extracellular matrix material (Rose and Turner, 1998 ; Körstgens et al, 2001 ). Previous studies have shown that the addition of Ca 2+ caused a significant increase in S. paucimobilis biofilm formation at different concentration levels (Guvensen et al, 2012 ). Another study has shown that Pseudoalteromonas spp .…”

Section: Discussionmentioning

confidence: 95%

Magnesium ions mitigate biofilm formation of Bacillus species via downregulation of matrix genes expression

2015

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The objective of this study was to investigate the effect of Mg2+ ions on biofilm formation by Bacillus species, which are considered as problematic microorganisms in the food industry. We found that magnesium ions are capable to inhibit significantly biofilm formation of Bacillus species at 50 mM concentration and higher. We further report that Mg2+ ions don't inhibit bacterial growth at elevated concentrations; hence, the mode of action of Mg2+ ions is apparently specific to inhibition of biofilm formation. Biofilm formation depends on the synthesis of extracellular matrix, whose production in Bacillus subtilis is specified by two major operons: the epsA-O and tapA operons. We analyzed the effect of Mg2+ ions on matrix gene expression using transcriptional fusions of the promoters for eps and tapA to the gene encoding β galactosidase. The expression of the two matrix operons was reduced drastically in response to Mg2+ ions suggesting about their inhibitory effect on expression of the matrix genes in B. subtilis. Since the matrix gene expression is tightly controlled by Spo0A dependent pathway, we conclude that Mg2+ ions could affect the signal transduction for biofilm formation through this pathway.

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Section: Discussionmentioning

confidence: 95%

Magnesium ions mitigate biofilm formation of Bacillus species via downregulation of matrix genes expression

2015

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“…Ca 2+ and Mg 2+ have other amazing properties. Particularly, it was shown that these cations can influence on bacterial biofilm formation (Guvensen et al 2012; Song and Leff 2006). These ions can also interact with antibiotics and protect the bacterial outer membrane from damage (Sahalan et al 2013).…”

Section: Discussionmentioning

confidence: 99%

Comparative analysis of the effect of Ca and Mg ions on antibacterial activity of lactic acid bacteria isolates and their associations depending on cultivation conditions

2019

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The effects of divalent cations of Ca and Mg on antibacterial activity of lactic acid bacteria (LAB) isolates, as well as their different associations were studied. Most LAB strains and associations revealed significant inhibitory effects in MRS against Gram-positive and Gram-negative test-organisms at different Ca 2+ and Mg 2+ concentrations (determined specifically for each LAB strain and each association). Some LAB strains and communities inhibited the growth of pathogenic test-organisms depending on both ions concentrations and cultivation conditions. Interestingly, the presence of Mg ions in medium significantly decreased the antimicrobial activity of LAB communities against pathogenic test-organisms; on the other hand, the combined mixture of ions essentially increased the inhibitory effect in case of time-spaced cultivation. In contrast, the inhibitory effects of many associations were significantly increased at the presence of Mg 2+ and especially ions combination in case of simultaneous cultivation. The addition of ions combination didn’t affect antibacterial activity of LAB isolates. The results allow us to conclude that Ca and Mg ions had inducible effects on antibacterial activity in case of simultaneous cultivation. This probably can be prospective for creation of new antimicrobial preparations and their possible application.

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“…Only sodium salts remain in the cooling water system, all of which are highly soluble. Low-calcium water has also been studied and shown to inhibit biofilm formation in cooling water systems [ 2 , 9 , 10 , 11 ].…”

Section: Pathogen Control Effects Of Natural Antimicrobial Chemistmentioning

confidence: 99%

“…Guvensen and Ozdeimer [ 11 ] report, that when grown in approximately 7500 mg/L soluble salt growth medium, biofilm formation on coupons increased proportionally to the addition of 0, 100, 250, and 500 μM (0, 4, 10, and 20 mg/L) Mg 2+ . Likewise, similar additions of Ca 2+ caused a marked increase in S. paucimobilis biofilm formation.…”

Section: Biofilm Inhibition By Polyvalent Metal Ion Concentration mentioning

confidence: 99%

Natural Pathogen Control Chemistry to Replace Toxic Treatment of Microbes and Biofilm in Cooling Towers

Brouse

Brouse²,

Brouse

2017

Pathogens

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Application of toxic antibacterial agents is considered necessary to control prevalent fresh water microorganisms that grow in evaporative cooling water systems, but can adversely affect the environment and human health. However, natural antibacterial water chemistry has been applied in industrial cooling water systems for over 10 years to inhibit microorganisms with excellent results. The water chemistry method concentrates natural minerals in highly-softened water to produce elevated pH and dissolved solids, while maintaining low calcium and magnesium content. The method provides further benefits in water conservation, and generates a small volume of non-toxic natural salt concentrate for cost efficient separation and disposal if required. This report describes the antimicrobial effects of these chemistry modifications in the cooling water environment and the resultant collective inhibition of microbes, biofilm, and pathogen growth. This article also presents a novel perspective of parasitic microbiome functional relationships, including “Trojan Protozoans” and biofilms, and the function of polyvalent metal ions in the formation and inhibition of biofilms. Reducing global dependence on toxic antibacterial agents discharged to the environment is an emerging concern due to their impact on the natural microbiome, plants, animals and humans. Concurrently, scientists have concluded that discharge of antibacterial agents plays a key role in development of pathogen resistance to antimicrobials as well as antibiotics. Use of natural antibacterial chemistry can play a key role in managing the cooling water environment in a more ecologically sustainable manner.

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Effects of magnesium and calcium cations on biofilm formation by Sphingomonas paucimobilis from an industrial environment

Cited by 17 publications

References 37 publications

Magnesium ions mitigate biofilm formation of Bacillus species via downregulation of matrix genes expression

Magnesium ions mitigate biofilm formation of Bacillus species via downregulation of matrix genes expression

Comparative analysis of the effect of Ca and Mg ions on antibacterial activity of lactic acid bacteria isolates and their associations depending on cultivation conditions

Natural Pathogen Control Chemistry to Replace Toxic Treatment of Microbes and Biofilm in Cooling Towers

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