Antimicrobial Resistance and Clinical Significant Resistance Mechanisms of Salmonella Isolated in 2014-2018 in ST.PETERSBURG, Russia

Sa, Egorova; Кафтырева, Л А; Сужаева, Л. В.; Забровская, А В; Войтенкова, Е. В.; Матвеева, З. Н.; Ostankova, Yu. V.; Likhachev, I. V.; Satosova, N. V.; Kitsbabashvili, R. V.; Smirnova, E. V.; Semchenkova, L. I.; Bystraya, T. E.; Sokol’nik, S. E.; Уткина, Н. П.; Sikhando, L. Y.

doi:10.18821/0869-2084-2019-64-10-620-626

Cited by 5 publications

(4 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Среди исследованных изолятов 16 (61,5%) были выделены от человека и характеризовались наиболь шим разнообразием серотипов (12), находки из про дуктов питания составляли 34,6% (3 серотипа), а из внешней среды был обнаружен всего 1 (3,8%) изолят S. Bovismorbificans. 25…”

Section: результатыunclassified

“…В ходе многолетнего мониторинга (2014-2018 гг.) антибио тикорезистентности штаммов сальмонелл, выделенных от детей и взрослых с диарейным синдромом в Санкт Петербурге, выявлена устойчивость к ЦРС у 12 штам мов (1,6%), включая ТЕМ1, БЛРС генетических групп СТХМ1, М2 и М9, и CMY2, что значительно больше, чем в 2003-2005 гг., когда при исследовании более 1000 штаммов только два из них были устойчивыми к ЦРС (0,2%) [25][26]. Полногеномное секвенирование и анализ 45 российских изолятов S. Infantis с множествен ной лекарственной устойчивостью (МЛУ) из образцов куриной и мясной продукции в течение 2018-2020 гг.…”

Section: Introductionunclassified

See 1 more Smart Citation

The prevalence and characterization of ESBL-producing strains of Salmonella enterica circulating in the territory of the Russian Federation (2016–2020)

Pavlova

Egorova

Krutova

et al. 2022

CMAC

View full text Add to dashboard Cite

Objective. To analyze frequency and identify genetic determinants of resistance of non-typhoid Salmonella (NTS) producing extended-spectrum β-lactamase (ESBL) isolated in the Russian Federation over the period 2016 to 2020. Materials and Methods. Salmonella isolates, suspected to ESBL production, were collected by the All-Russia Reference Center of Salmonellosis during the national Salmonellosis surveillance program. Phenotypic resistance was determined by the broth microdilution method using G-I and G-II Mikrolatest®SensiLaTest MIC plates and by the double-disk synergy test. Whole genome sequencing was performed on the NextSeq platform (Illumina, USA), with subsequent de novo genome assembly (SPAdes 3.15.4), identification of plasmid types (MOB-suite v3.0.0), and identification of resistance genes (AMRFinderPlus v3.10.40). Results. Out of 1792 NTS isolates, 22 strains contained bla-genes of molecular classes A and D (blaTEM, blaCTX-M, blaSHV, blaOXA), one strain – AmpC (blaCMY-2) and three strains – combination ESBL of class A and AmpC (blaTEM, blaCMY-2, blaDHA). The frequency of occurrence of ESBL-producing Salmonella is 1.3%, AmpC – 0.2%. Additionally, strains were resistant to other non-β-lactam antibiotics. Six different types of plasmids were identified (IncI, IncFIB, IncC, IncHI2A, IncL/M and IncX1) in studied isolates. It was possible for 17 strains to identify location of resistance genes in plasmids of a certain type. Conclusions. The frequency of occurrence of Salmonella strains producing ESBL and AmpC was 1.45%, which were found in sporadic cases of human diseases, as well as food and environmental objects were sources of isolation. The fact of detection of such strains among various NTC serotypes and a wide range of sources of isolation confirms the relevance of monitoring antimicrobial resistance of Salmonella strains in the future.

show abstract

Section: результатыunclassified

Section: Introductionunclassified

The prevalence and characterization of ESBL-producing strains of Salmonella enterica circulating in the territory of the Russian Federation (2016–2020)

Pavlova

Egorova

Krutova

et al. 2022

CMAC

View full text Add to dashboard Cite

show abstract

“…This is because current standards do not regulate the antibiogram guidelines for salmonellosis, and empiric therapy is commonly used. An analysis of the available literature, however, shows an increase in the drug resistance of Salmonella strains to antimicrobial agents in different regions of Russia and worldwide due to misuse [ 2 , 3 ]. Increasing antimicrobial resistance (AMR) and the emergence of multiple drug resistant (MDR) Salmonella strains have increased the need to understand the susceptibility of Salmonella strains that are currently circulating in different countries and their administrative regions to antimicrobial drugs for adequate etiotropic therapy [ 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

Antibiotic Resistance and Plasmid Profile Analysis of Salmonella Enteritidis Isolated in Siberia and the Far East of Russia between 1990 and 2017

Rakov

Kuznetsova

2021

Pathogens

View full text Add to dashboard Cite

Salmonella is one of the major causes of foodborne disease outbreaks globally. Specifically, Salmonella enterica subsp. enterica serovar Enteritidis (S. Enteritidis) is one of the major causes of zoonotic Salmonella infection in humans worldwide. In this study, we present data on antimicrobial resistance (AMR) and plasmid profiles of S. Enteritidis strains isolated from patients, food, and the environment in Siberia and the Far East of Russia obtained during Salmonella monitoring between 1990 and 2017. A total of 345 S. Enteritidis isolates were tested by the disk diffusion method with a set of 15 antibiotics using EUCAST breakpoints v. 10 and by plasmid profile analysis using the alkaline lysis method. The results have shown a substantial decrease in susceptibility to aminoglycosides and quinolones during the study period. No significant differences were found in the susceptibility of strains between regions as well as in the its correlation with different plasmid types of the pathogen. Several S. Enteritidis strains were found to be resistant to ampicillin, kanamycin, tetracycline, chloramphenicol, and cephalosporins. All tested S. Enteritidis strains were susceptible only to imipenem. In this study, we observed a relatively low level of AMR in S. Enteritidis strains isolated in Siberia and the Far East of Russia. Nevertheless, it is important to continue the molecular genetic monitoring and AMR surveillance of S. Enteritidis to track further increases in AMR using conventional phenotypic susceptibility testing and by introducing whole-genome sequencing to identify AMR mechanisms.

show abstract

“…Фторхинолоны являются препаратами выбора при лечении тяжелых форм сальмонеллезных инфекций, включая брюшной тиф. В то же время, по данным раз личных авторов, в Российской Федерации доля штаммов Salmonella, устойчивых к этому классу антимикробных препаратов (АМП) достигает 50-70% [1][2][3][4]. У штаммов Salmonella описаны хромосомные и плазмидоопосредо ванные механизмы устойчивости к хинолонам.…”

Section: Introductionunclassified

Methodological approaches to fluoroquinolone susceptibility testing of Salmonella

Egorova

Кафтырева

2020

CMAC

Self Cite

View full text Add to dashboard Cite

Objective. To evaluate effectiveness of the various approaches to fluoroquinolone susceptibility testing of Salmonella, including S. typhi, taking into account molecular resistance mechanisms. Materials and Methods. The MIC values of ciprofloxacin were compared with the inhibition zone for pefloxacin disc, 5 mg (310 isolates) and nalidixic acid, 30 mg (420 isolates). MIC of ciprofloxacin was determined by gradient diffusion test and broth microdilution method. Muller – Hinton agar and Muller – Hinton broth, antibiotic discs and MICE-tests (Oxoid, UK) were used. Chromosomal mutations in QRDR of gyrA, gyrB, parC, and parE genes and plasmid-mediated quinolone resistance genes (qnr, aac-(6’)-1b etc.) were detected in 19 Salmonella isolates by analysis using ResFinder service (Center of Genomic Epidemiology). Genomic DNA libraries were prepared using the MiSeq Nextera XT Library Preparation Kit (Illumina, USA). WGS was performed on MiSeq (Illumina, USA) with MiSeq Reagent Kit v3 600 cycles (Illumina, USA). Genome assembly and analysis were performed using CLC Genomics Workbench 8.0 (Qiagen, USA). Results. Despite the high concordance of ciprofloxacin MIC values and the results of disc diffusion screening with pefloxacin (96.5% of isolates) and nalidixic acid (98.1% of isolates), the results obtained for some resistant isolates were inconsistent. When those isolates were tested by a single method, there was a possibility of incorrect susceptibility categorization. Discordant results were obtained for 19 isolates and had the objective reason (paradoxical resistance phenotype due to the plasmid-mediated resistance, qnrS) in 3 cases. Other discrepancies were noted when the values were equal to the clinical breakpoints: ciprofloxacin MIC – 0.06 mg/l, inhibition zone for pefloxacin – 24 mm. Repeated testing revealed the variations around the clinical breakpoints: the MIC values of 0.06–0.12 mg/l, and inhibition zone of 23 to 25 mm. Conclusions. When performing fluoroquinolone susceptibility testing of Salmonella, it is reasonable to add the category “Area of Technical Uncertainty”: ciprofloxacin MIC value of 0.06 mg/l, and inhibition zone for pefloxacin of 23 to 25 mm. Two discs (pefloxacin and nalidixic acid) should be used for fluoroquinolone resistance screening by disk diffusion method.

show abstract

Antimicrobial Resistance and Clinical Significant Resistance Mechanisms of Salmonella Isolated in 2014-2018 in ST.PETERSBURG, Russia

Cited by 5 publications

References 12 publications

The prevalence and characterization of ESBL-producing strains of Salmonella enterica circulating in the territory of the Russian Federation (2016–2020)

The prevalence and characterization of ESBL-producing strains of Salmonella enterica circulating in the territory of the Russian Federation (2016–2020)

Antibiotic Resistance and Plasmid Profile Analysis of Salmonella Enteritidis Isolated in Siberia and the Far East of Russia between 1990 and 2017

Methodological approaches to fluoroquinolone susceptibility testing of Salmonella

Contact Info

Product

Resources

About