Antimicrobial resistance and genotypic relatedness of environmental staphylococci in semi-extensive dairy farms

Monte, Daniel Farias Marinho do; Júnior, Wellington Dias Lopes; Abley, Melanie; Gebreyes, Wondwossen A.; Oliveira, Celso José Bruno de

doi:10.1016/j.vas.2018.07.007

Cited by 11 publications

(9 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some studies use resistance to oxacillin [59,65] or cefoxitin [62,64] or both [25] to determine if the strain is MRSA. It is also possible to determine MIC of S. aureus strains by the E-test according to the protocol suggested by the manufacturer [61].…”

Section: Methods Used For Identification and Characterization Of Mrsamentioning

confidence: 99%

“…Identification by polymerase chain reaction (PCR) [41,59,[62][63][64][65][66][67][68] is also widely used. Specie confirmation can be performed using nuc gene; mecA gene is used to identify the methicillin-resistant strain [59,62,63,65,67,68]. Other alternative for detection of MRSA is the use of kits based on PCR, a multiplex PCR and real-time PCR, to detect each SCCmec type and the chromosomal orfX-SCCmec junction [69].…”

Section: Methods Used For Identification and Characterization Of Mrsamentioning

confidence: 99%

“…After confirmation of the mecA gene, it is important to submit the strains to additional molecular characterization. The techniques that are generally used are as follows: staphylococcal protein A (spa) typing [59,61,[66][67][68], multilocus sequence typing (MLST) [59,61,64,66,67,70], pulsed field gel electrophoresis (PFGE) [64][65][66], Staphylococcal cassette chromosome mec (SCCmec) typing [59,66].…”

Section: Methods Used For Identification and Characterization Of Mrsamentioning

confidence: 99%

“…Researches in different regions have been showing the incidence of MRSA in food and foodproducing animals, with an emphasis on milk and dairy products and meat and meat products. Furthermore, the importance of researches conducted with samples of food processing environments and food handlers should also be highlighted [65,89,90]. Next, studies shall be presented to demonstrate the incidence of S. aureus and specifically MRSA in Brazilian territory.…”

Section: Mrsa In Brazilmentioning

confidence: 99%

See 3 more Smart Citations

Methicillin-resistant Staphylococcus aureus in food and the prevalence in Brazil: a review

2019

View full text Add to dashboard Cite

Foodborne diseases (FBD) occur worldwide and affect a large part of the population, being a cause of international concern among health authorities. Staphylococcus aureus can be transmitted by contaminated food, and it is one of the pathogens that most cause foodborne outbreaks in Brazil. Currently, this organism's ability in developing resistance to antibiotics is notorious; methicillin-resistant Staphylococcus aureus-MRSA-is known for its resistance to methicillin, oxacillin, and others. MRSA is one of the leading causes of infections, becoming a major threat to human health worldwide due to the numerous toxins that can produce. At first, the transmission of MRSA occurred in clinical environments; but in recent decades, its presence has been reported in the community, outside the hospital environment, including food and food-producing animals around the world. In this review, information about MRSA was gathered to verify MRSA incidence in the world but especially in Brazil in food samples, food handlers, food-producing animals, and food processing environments. The studies show that MRSA is easily found and in certain cases with high frequency, thus representing a potential risk to public health.

show abstract

Section: Methods Used For Identification and Characterization Of Mrsamentioning

confidence: 99%

Section: Methods Used For Identification and Characterization Of Mrsamentioning

confidence: 99%

Section: Methods Used For Identification and Characterization Of Mrsamentioning

confidence: 99%

Section: Mrsa In Brazilmentioning

confidence: 99%

See 2 more Smart Citations

Methicillin-resistant Staphylococcus aureus in food and the prevalence in Brazil: a review

2019

View full text Add to dashboard Cite

show abstract

“…It has been reported that humans sometimes consume raw milk [16]. Nowadays, livestock-associated and community-associated MRSA have been reported and are of great public health concern [17][18][19][20]. The consumption of contaminated milk and milk products was reported to be a source of infections by S. aureus [21].…”

Section: Introductionmentioning

confidence: 99%

Molecular Detection of Multidrug Resistant Staphylococcus aureus Isolated from Bovine Mastitis Milk in Bangladesh

Salauddin

Akter

Hossain

et al. 2020

Veterinary Sciences

View full text Add to dashboard Cite

The current study was conducted to isolate and identify multidrug-resistant Staphylococcus aureus (MDR-SA) from mastitis milk samples and to determine their antimicrobial susceptibility pattern. A total of 48 bovine mastitis (BM) milk samples were collected from different parts of the Rangpur division, Bangladesh. After the collection of milk samples, mastitis was confirmed using the California mastitis test. Isolation and identification of Staphylococcus aureus were performed using conventional cultural and biochemical tests as well as using molecular methods of PCR. Nucleotide sequence analysis of the 23S rRNA gene of Staphylococcus aureus was determined. The antibiogram of the isolated bacteria was conducted using the disc diffusion method. Phylogenetic analysis of 23S rRNA was done using MEGA 7, ClustalW multiple sequence alignment, and NCBI-BLAST tools, where the sequence of the isolate showed 98% to 99% identity. Antibiogram test using 15 antimicrobial agents showed that all of the Staphylococcus aureus isolates were classified as multidrug-resistant (MDR). It was found that the isolates were resistant to tetracycline, novobiocin, methicillin, vancomycin, and cephradine, and the isolates were sensitive to ciprofloxacin, azithromycin, norfloxacin, levofloxacin, gentamicin, and amoxicillin. The detection of MDR-SA in mastitis milk is alarming and represents a great public health concern. The findings of the present study help identify Staphylococcus aureus at the molecular level using 23S rRNA gene sequencing and will help select the appropriate and effective antimicrobial agent to control BM in the northern part of Bangladesh.

show abstract

Milk supply chain as a reservoir of antimicrobial-resistant Staphylococcus species

Supriya,

Karabasanavar,

Madhavaprasad

et al. 2024

J Biosci

View full text Add to dashboard Cite

Antimicrobial resistance and genotypic relatedness of environmental staphylococci in semi-extensive dairy farms

Cited by 11 publications

References 14 publications

Methicillin-resistant Staphylococcus aureus in food and the prevalence in Brazil: a review

Methicillin-resistant Staphylococcus aureus in food and the prevalence in Brazil: a review

Molecular Detection of Multidrug Resistant Staphylococcus aureus Isolated from Bovine Mastitis Milk in Bangladesh

Milk supply chain as a reservoir of antimicrobial-resistant Staphylococcus species

Contact Info

Product

Resources

About