Antimicrobial resistance and molecular epidemiology of vancomycin-resistant enterococci from North America and Europe: a report from the SENTRY antimicrobial surveillance program

Deshpande, Lalitagauri M.; Fritsche, Thomas R.; Moet, Gary J.; Biedenbach, Douglas J.; Jones, Ronald N.

doi:10.1016/j.diagmicrobio.2006.12.022

Cited by 292 publications

(220 citation statements)

References 33 publications

Supporting

Mentioning

192

Contrasting

Unclassified

Order By: Relevance

“…Before 2004, only two VRE had been isolated in the Bacteriology laboratory of the Clinic for Infectious and Tropical Diseases at the CCS; the increasing number of these bacteria from our hospitals after that period is consistent with the fi nding of increasing isolation of VRE in Europe [12], and the introduction of a screening technique for VRE of carrier origin. The greatest number of VRE originated from the CCS, which is the teaching hospital and where seriously ill patients from all over Serbia and the surrounding states of ex-Yugoslavia are sent for treatment.…”

Section: Discussionsupporting

confidence: 61%

Molecular typing, pathogenicity factor genes and antimicrobial susceptibility of vancomycin resistant enterococci in Belgrade, Serbia

Jovanović¹,

Milošević

Tošić³

et al. 2015

Acta Microbiologica et Immunologica Hungarica

View full text Add to dashboard Cite

In this study the distribution of species and antimicrobial resistance among vancomycin resistant enterococci (VRE) recovered from clinical specimens obtained from fi ve hospitals in Belgrade was analyzed. Strains were further characterized by pulsed-fi eld gel electrophoresis (PFGE). Polymerase chain reaction (PCR) was used to investigate the presence of vanA and vanB genes and pathogenicity factor genes. Identifi cation of 194 VRE isolates revealed 154 Enterococcus faecium, 21 Enterococcus faecalis, 10 Enterococcus raffi nosus and 9 Enterococcus gallinarum. This study revealed existence of 8 major clones of VRE. PCR determined vanA gene to be present in all of the VRE studied. Esp and hyl genes were present in 29.22% and 27.92% of E. faecium, respectively, and in 76.19% and 0 of E. faecalis, respectively. Esp and hyl genes were not found more frequently in members of predominant clones of E. faecium than in single isolates; nor was their presence connected to invasiveness.

show abstract

Section: Discussionsupporting

confidence: 61%

Molecular typing, pathogenicity factor genes and antimicrobial susceptibility of vancomycin resistant enterococci in Belgrade, Serbia

Jovanović¹,

Milošević

Tošić³

et al. 2015

Acta Microbiologica et Immunologica Hungarica

View full text Add to dashboard Cite

show abstract

“…In addition, all VREfm isolates during this study were uniformly susceptible to both drugs. A similar chloramphenicol resistance rate among VREfm of 0.5% was observed in North America 21 ; however, chloramphenicol use for the treatment of VRE infections is known to result in the development of resistance 30 .…”

Section: Conflict Of Interest Financial Support Referencesmentioning

confidence: 56%

“…Molecular epidemiological studies have reported the spread of a hospital-adapted complex of E. faecium designated as clonal complex-17 (CC-17), which is associated with the majority of hospitals outbreaks and clinical infections on all continents 21,22 . This complex is characterized by ampicillin and quinolones resistance and by the presence of a putative pathogenicity island.…”

Section: Conflict Of Interest Financial Support Referencesmentioning

confidence: 99%

Trends in antimicrobial resistance among clinical isolates of enterococci in a Brazilian tertiary hospital: a 4-year study

Conceição

Oliveira

Silva

et al. 2011

Rev. Soc. Bras. Med. Trop.

View full text Add to dashboard Cite

Introduction: In the past two decades members of the genus Enterococcus have emerged as important nosocomial pathogens worldwide. This study prospectively analyzed the distribution of species and trends in antimicrobial resistance among clinical isolates of enterococci in a Brazilian tertiary hospital from 2006-2009. Methods: Enterococcal species were identified by conventional biochemical tests. The antimicrobial susceptibility profile was performed by disk diffusion in accordance with the Clinical and Laboratory Standards Institute (CLSI). A screening test for vancomycin was also performed. Minimal inhibitory concentration (MIC) for vancomycin was determined using the broth dilution method. Molecular assays were used to confirm speciation and genotype of vancomycin-resistant enterococci (VRE). Results: A total of 324 non-repetitive enterococcal isolates were recovered, of which 87% were E. faecalis and 10.8% E. faecium. The incidence of E. faecium per 1,000 admissions increased significantly (p < 0.001) from 0.3 in 2006 to 2.3 in 2009. The VRE rate also increased over time from 2.5% to 15.5% (p < 0.001). All VRE expressed high-level resistance to vancomycin (MIC >256μg/ mL) and harbored vanA genes. The majority (89.5%) of VRE belonged to E. faecium species, which were characteristically resistant to ampicillin and quinolones. Overall, ampicillin resistance rate increased significantly from 2.5% to 21.4% from 2006-2009. Resistance rates for gentamicin, chloramphenicol, tetracycline, and erythromycin significantly decreased over time, although they remained high. Quinolones resistance rates were high and did not change significantly over time. Conclusions: The data obtained show a significant increasing trend in the incidence of E. faecium resistant to ampicillin and vancomycin.

show abstract

“…Vancomycin has been used as the last resort for the treatment of infectious diseases caused by S. aureus and enterococci; however, since 1986, VRE strains have emerged and become widespread. 4,5 Since the beginning of this century, vancomycin intermediate-resistant S. aureus have been frequently isolated 6 and 33 strains of vancomycin high-level resistant S. aureus have also emerged in Asian countries and in the United States. 7 Effective drugs against these pathogens are urgently needed.…”

mentioning

confidence: 99%

Structure and antibacterial activities of new cyclic peptide antibiotics, pargamicins B, C and D, from Amycolatopsis sp. ML1-hF4

et al. 2017

View full text Add to dashboard Cite

Antimicrobial resistance and molecular epidemiology of vancomycin-resistant enterococci from North America and Europe: a report from the SENTRY antimicrobial surveillance program

Cited by 292 publications

References 33 publications

Molecular typing, pathogenicity factor genes and antimicrobial susceptibility of vancomycin resistant enterococci in Belgrade, Serbia

Molecular typing, pathogenicity factor genes and antimicrobial susceptibility of vancomycin resistant enterococci in Belgrade, Serbia

Trends in antimicrobial resistance among clinical isolates of enterococci in a Brazilian tertiary hospital: a 4-year study

Structure and antibacterial activities of new cyclic peptide antibiotics, pargamicins B, C and D, from Amycolatopsis sp. ML1-hF4

Contact Info

Product

Resources

About