Antimicrobial resistance, biofilm production and invasion of mammary epithelial cells by Enterococcus faecalis and Enterococcus mundtii strains isolated from bovine subclinical mastitis in Brazil

Rodrigues, Dayane de Souza; Lannes-Costa, Pamella Silva; Santos, Gabriela da Silva; Ribeiro, Rachel Leite; Langoni, Hélio; Teixeira, Lúcia Martins; Nagao, Prescilla Emy

doi:10.1111/lam.13718

Letters in Applied Microbiology

2022

DOI: 10.1111/lam.13718

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Antimicrobial resistance, biofilm production and invasion of mammary epithelial cells by Enterococcus faecalis and Enterococcus mundtii strains isolated from bovine subclinical mastitis in Brazil

Dayane de Souza Rodrigues

Pamella Silva Lannes-Costa

Gabriela da Silva Santos

et al.

Abstract: Significance and impact of the study: Bacterial strains isolated from bovine subclinical mastitis were identified as Enterococcus faecalis and Enterococcus mundtii. Enterococcus faecalis and E. mundtii strains recovered from different farms were mostly clonal, suggesting possible cross-transmission among animals in the region. Biofilms were produced by E. faecalis and E. mundtii strains grown in milk. Rearrangement of the actin cytoskeleton in host epithelial cells was induced by E. faecalis and E. mundtii str… Show more

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Cited by 2 publications

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Enterococcus faecalis : an overlooked cell invader

Archambaud,

Nunez,

da Silva

et al. 2024

Microbiol Mol Biol Rev

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SUMMARY Enterococcus faecalis and Enterococcus faecium are human pathobionts that exhibit a dual lifestyle as commensal and pathogenic bacteria. The pathogenic lifestyle is associated with specific conditions involving host susceptibility and intestinal overgrowth or the use of a medical device. Although the virulence of E. faecium appears to benefit from its antimicrobial resistance, E. faecalis is recognized for its higher pathogenic potential. E. faecalis has long been considered a predominantly extracellular pathogen; it adheres to and is taken up by a wide range of mammalian cells, albeit with less efficiency than classical intracellular enteropathogens. Carbohydrate structures, rather than proteinaceous moieties, are likely to be primarily involved in the adhesion of E. faecalis to epithelial cells. Consistently, few adhesins have been implicated in the adhesion of E. faecalis to epithelial cells. On the host side, very little is known about cognate receptors, except for the role of glycosaminoglycans during macrophage infection. Several lines of evidence indicate that E. faecalis internalization may involve a zipper-like mechanism as well as a macropinocytosis pathway. Conversely, E. faecalis can use several strategies to prevent engulfment in phagocytes. However, the bacterial and host mechanisms underlying cell infection by E. faecalis are still in their infancy. The most recent striking finding is the existence of an intracellular lifestyle where E. faecalis can replicate within a variety of host cells. In this review, we summarize and discuss the current knowledge of E. faecalis –host cell interactions and argue on the need for further mechanistic studies to prevent or reduce infections.

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Enterococcus faecalis : an overlooked cell invader

Archambaud,

Nunez,

da Silva

et al. 2024

Microbiol Mol Biol Rev

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Effect of the Presence of Antibiotic Residues on the Microbiological Quality and Antimicrobial Resistance in Fresh Goat Meat

Silva-Guedes

Martínez‐Laorden

González‐Fandos

2022

Foods

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A total of 11 fresh goat legs were collected at the retail level. Mesophiles, Pseudomonas spp., Enterobacteriaceae, staphylococci, enterococci, Clostridium perfringens, Campylobacter spp., and Listeria monocytogenes counts were determined. Nine samples were free of antibiotic residues, while in the other two samples the presence of sulfadiazine and doxycycline was detected. The antimicrobial resistance of E. coli, staphylococci, Macrococcus spp., and enterococci isolates was also evaluated. Clostridium perfringens was found in two samples. Methicillin-resistant Staphylococcus aureus was detected in one sample. S. epidermidis isolated from one sample containing doxycycline residues showed resistance to mupirocin. Moreover, multi-resistant S. epidermidis and M. caseolyticus were found. Most of the isolated Enterococcus faecium were multi-resistant. Neither extended-spectrum β-lactamase -producing E. coli nor vancomycin-resistant enterococci were detected in any sample. The presence of doxycycline or sulfadiazine could affect the goat meat microbiota since less microbial diversity was found in these samples compared to those free of antibiotics. The presence of antibiotic residues could increase the antimicrobial resistance of enterococci in fresh goat meat. The presence of multidrug-resistant bacteria in goat meat could be considered a potential threat and should be monitored. Special measures should be taken at the farm level and during slaughter to reduce antimicrobial resistance.

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Antimicrobial resistance, biofilm production and invasion of mammary epithelial cells by Enterococcus faecalis and Enterococcus mundtii strains isolated from bovine subclinical mastitis in Brazil

Cited by 2 publications

References 39 publications

Enterococcus faecalis : an overlooked cell invader

Enterococcus faecalis : an overlooked cell invader

Effect of the Presence of Antibiotic Residues on the Microbiological Quality and Antimicrobial Resistance in Fresh Goat Meat

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