Antimicrobial resistance in Escherichia coli isolates from frugivorous (Eidolon helvum) and insectivorous (Nycteris hispida) bats in Southeast Nigeria, with detection of CTX-M-15 producing isolates

Obodoechi, Lynda Onyinye; Carvalho, Isabel; Chenouf, Nadia Safia; Martínez-Álvarez, Sandra; Sadi, Madjid; Nwanta, John A.; Chah, Kennedy F.; Torres, Carmén

doi:10.1016/j.cimid.2021.101613

Cited by 14 publications

(21 citation statements)

References 67 publications

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“…Antimicrobial therapy is the method of choice to treat bacterial infections, but the continuous emergence and spread of MDR strains in recent years has compromised the effectiveness of antimicrobial drugs. 19 E. coli is one of the most commonly isolated bacteria clinically, but its increasing resistance to antimicrobials in recent years is making treatment more difficult. 20 In this study, we collected clinical E. coli isolates in our hospital over a 3-month period.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Resistance and Molecular Characterization of Gene Cassettes from Class 1 Integrons inEscherichia coliStrains

Sun

et al. 2022

Microbial Drug Resistance

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To investigate the antimicrobial resistance and molecular characterization of gene cassettes from class 1 integrons in Escherichia coli strains isolated from hospitalized patients. Bacterial identification was conducted using the Vitek-2 Compact system, and antimicrobial susceptibility analysis was performed using the Kirby-Bauer method. Class 1 integrons, integrase genes, the variable regions of integrons and promoters from the isolated E. coli were screened by polymerase chain reaction, and subjected to DNA sequencing. In total, 138 E. coli strains were collected from the hospitalized patients, most from urine specimens (41.30%, 57/138). Antimicrobial resistance to ampicillin (89.86%) was most prevalent, with 79.99% of strains being multidrug-resistant (MDR). The class 1 integron integrase intI1 gene was detected in 67.39% of the isolates (93/138). Three gene cassette arrays and 5 antimicrobial resistance gene cassettes were detected in 69 of the class 1 integron-positive strains. The most common gene cassette array was dfrA17-aadA5 . Of the 93 intI1 -positive strains, 5 different common promoters were detected. The most prevalent common promoter was PcH1, and most isolates contained the dfrA17-aadA5 gene cassette array. In summary, antimicrobial resistance and MDR were prevalent among E. coli isolates in our city Weifang in Shandong Provence China. Gene cassettes of the class 1 integron variable region mostly conferred resistance to traditional antimicrobials. Weak promoters in the variable regions were predominant in this study. Integrons pose a great threat to the treatment of MDR bacterial infections and further investigations are needed.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial Resistance and Molecular Characterization of Gene Cassettes from Class 1 Integrons inEscherichia coliStrains

Sun

et al. 2022

Microbial Drug Resistance

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“…Pets can also be reservoirs of STEC and enteropathogenic E. coli (EPEC) strains. Recent studies have reported its presence among E. coli isolated from wildlife animals [14,26].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Resistance Genes and Diversity of Clones among ESBL- and Acquired AmpC-Producing Escherichia coli Isolated from Fecal Samples of Healthy and Sick Cats in Portugal

et al. 2021

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The aim of the study was to analyze the mechanisms of resistance in extended-spectrum beta-lactamase (ESBL)- and acquired AmpC (qAmpC)-producing Escherichia coli isolates from healthy and sick cats in Portugal. A total of 141 rectal swabs recovered from 98 sick and 43 healthy cats were processed for cefotaxime-resistant (CTXR) E. coli recovery (in MacConkey agar supplemented with 2 µg/mL cefotaxime). The matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) method was used for E. coli identification and antimicrobial susceptibility was performed by a disk diffusion test. The presence of resistance/virulence genes was tested by PCR sequencing. The phylogenetic typing and multilocus sequence typing (MLST) were determined by specific PCR sequencing. CTXRE. coli isolates were detected in seven sick and six healthy cats (7.1% and 13.9%, respectively). Based on the synergy tests, 11 of 13 CTXRE. coli isolates (one/sample) were ESBL-producers (ESBL total rate: 7.8%) carrying the following ESBL genes: blaCTX-M-1 (n = 3), blaCTX-M-15 (n = 3), blaCTX-M-55 (n = 2), blaCTX-M-27 (n = 2) and blaCTX-M-9 (n = 1). Six different sequence types were identified among ESBL-producers (sequence type/associated ESBLs): ST847/CTX-M-9, CTX-M-27, CTX-M-1; ST10/CTX-M-15, CTX-M-27; ST6448/CTX-M-15, CTX-M-55; ST429/CTX-M-15; ST101/CTX-M-1 and ST40/CTX-M-1. Three of the CTXR isolates were CMY-2-producers (qAmpC rate: 2.1%); two of them were ESBL-positive and one ESBL-negative. These isolates were typed as ST429 and ST6448 and were obtained in healthy or sick cats. The phylogenetic groups A/B1/D/clade 1 were detected among ESBL- and qAmpC-producing isolates. Cats are carriers of qAmpC (CMY-2)- and ESBL-producing E. coli isolates (mostly of variants of CTX-M group 1) of diverse clonal lineages, which might represent a public health problem due to the proximity of cats with humans regarding a One Health perspective.

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“…In one study, the specie was not determined. (Walther et al, 2008) The type of sample predominately was fecal material (anal swabs, droplets, and gastrointestinal samples) in almost every paper, except for two works that had samples from other organs (Walther et al, 2008;Obodoechi et al, 2021). The countries with the greatest number of studies were in decedent order as follows: Nigeria, Brazil (n=5), Slovakia, Australia (n = 3), Indonesia, Peru, Portugal, Poland, Algeria (n = 2), Japan, Republic of Congo, Slovakia, Trinidad, Germany, Bangladesh, Gabon (n = 1).…”

Section: Antibiotic Resistance In Chiropteransmentioning

confidence: 99%

“…A study performed by Obodoechi et al (2021), detected the blaCTX-M15 gene in ESBL isolated from bats from Nigeria. This gene already had been detected in other bats from Gabon (Nguema et al, 2020), Poland (Nowakiewicz et al, 2020), and Peru (Benavides et al, 2018;Mendoza, 2017).…”

Section: Antibiotic Resistance In Chiropteransmentioning

confidence: 99%

Bats and Antibiotic Resistance: A Culprit or a Victim?

Garcês¹

2022

WVJ

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In the last decades, the increase of antimicrobial resistance bacteria has become a concern for public health. Bats’ ability to fly, form colonies for a long lifespan, and inhabit a variety of diverse ecological niches make them successful species in terms of adaptation and distribution on earth. Moreover, these characteristics let them act as the potential natural reservoir of numerous zoonotic pathogens (bacteria, viruses, fungi). Bat bacteriome knowledge is still very scarce, but a few studies have indicated that bats are hosts of antimicrobial resistance and play an important role in the dispersion of resistance in the environment. Moreover, bats are vulnerable to acquiring these pathogens since they sometimes live in close contact with humans and domestic animals. Therefore, the present study aimed to compile the latest studies that describe the presence of antibiotic-resistant in bats. Based on the papers analyzed for this review, it is possible to conclude that bats are hosts of pathogenic bacteria that carry numerous antibiotic resistance. Extended-spectrum b-lactamases (ESBLs) or Methicillin-resistant Staphylococcus which nowadays days are a great public health concern, have already been reported in these animals, with some isolated strains being of Human origin. Although not completely understood regarding the dynamics and transmission routes, bats seem to have an important role in the dissemination and acquisition of antibiotic resistance in the environment. They can be contaminated by bacteria with antibiotic resistance and disperse through the environment. However, they also can be the host of bacteria that carry antibiotic resistance.

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Antimicrobial resistance in Escherichia coli isolates from frugivorous (Eidolon helvum) and insectivorous (Nycteris hispida) bats in Southeast Nigeria, with detection of CTX-M-15 producing isolates

Cited by 14 publications

References 67 publications

Antimicrobial Resistance and Molecular Characterization of Gene Cassettes from Class 1 Integrons inEscherichia coliStrains

Antimicrobial Resistance and Molecular Characterization of Gene Cassettes from Class 1 Integrons inEscherichia coliStrains

Antimicrobial Resistance Genes and Diversity of Clones among ESBL- and Acquired AmpC-Producing Escherichia coli Isolated from Fecal Samples of Healthy and Sick Cats in Portugal

Bats and Antibiotic Resistance: A Culprit or a Victim?

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