Antimicrobial resistance modulation of MDR E. coli by antibiotic coated ZnO nanoparticles

Anwar, Muhammad; Aqib, Amjad Islam; Ashfaq, Khurram; Deeba, Farrah; Khan, Muhammad Kashif Iqbal; Khan, Shanza Rauf; Muzammil, Iqra; Shoaib, Muhammad; Naseer, Muhammad Aamir; Riaz, Tanzeel; Tanveer, Qaisar; Sadiq, Masood; Lodhi, Fizzah Laeeq; Ashraf, Fozia

doi:10.1016/j.micpath.2020.104450

Cited by 19 publications

(27 citation statements)

References 34 publications

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“…Antimicrobial resistance modulation by combination of antibiotics with non-steroidal anti-inflammatory drugs [ 29 , 30 , 62 ], plant extracts [ 63 , 64 ], and nano-particles [ 65 , 66 ] is well-documented with promising results. The combination of penicillin and gentamicin resulted in a 61% reduction in the MIC value of penicillin with a 100% cure rate against drug-resistant pathogens [ 67 ].…”

Section: Discussionmentioning

confidence: 99%

“…Antibiotics to be tested (alone or in combination) were poured into the wells to assess their antibacterial potential. Overnight incubation at 37 °C was followed by measuring of zones of inhibition to determine synergistic drug combinations [ 29 ].…”

Section: Methodsmentioning

confidence: 99%

“…Inhibitory concentration indices were measured as per the formula given below. To avoid errors, the experiment was performed in triplicate [ 29 , 30 ].…”

Section: Methodsmentioning

confidence: 99%

“…Tukey test as a post hoc test following ANOVA was applied to find significant differences among different groups. Percentage increase in zone sizes and fractional inhibitory indices were calculated using the prescribed formulas [ 29 , 30 ]. The statistical analysis was carried out using the computer-based statistical program SPSS (version 20) at a 5% probability level.…”

Section: Methodsmentioning

confidence: 99%

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Evidence-Based Tracking of MDR E. coli from Bovine Endometritis and Its Elimination by Effective Novel Therapeutics

Shafique

Aqib

et al. 2021

Antibiotics

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Antibiotic-resistant bacteria have become the predominant etiology of endometritis and thus require effective treatment approaches. We used ultrasonography coupled with clinical signs and presented complaints of reproductive issues to investigate the epidemiology, phylogenetic analysis, antimicrobial resistance, and development of novel therapeutics against Escherichia coli isolated from endometritis in bovine (n = 304 from 10 commercial dairy farms). The prevalence of bovine endometritis in this study was 43.75%, while among these, 72.18% samples were positive for E. coli. Nucleotide analysis performed through BLAST and MEGAX showed 98% similarity to the nucleotide sequence of the reference E. coli strain (accession number CP067311.1). The disk diffusion assay revealed pathogen resistance to most antibiotics. Pattern of MIC order of resistance was as follows: enrofloxacin < gentamicin < co-amoxiclav < streptomycin < amoxicillin < metronidazole < oxytetracycline. Field trials revealed the highest recovery rate (in terms of clearance of endometritis and establishment of pregnancy) in case of gentamicin + enrofloxacin (100%) and gentamicin alone (100%), followed by co-amoxiclav + gentamicin (84.61%), oxytetracycline alone (78.57%), and metronidazole + enrofloxacin (33.33%). Hence, the current study reported a higher prevalence of multidrug-resistant E. coli showing considerable similarity with reference strain, and finally, the effective response of novel antibiotics to treat cases.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Inhibitory concentration indices were measured as per the formula given below. To avoid errors, the experiment was performed in triplicate [ 29 , 30 ].…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Evidence-Based Tracking of MDR E. coli from Bovine Endometritis and Its Elimination by Effective Novel Therapeutics

Shafique

Aqib

et al. 2021

Antibiotics

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“…Mastitis is caused by multiple bacterial etiologies, where E. coli is known as one of the most significant causes of clinical mastitis in dairy animals, typically occurred in high producing cows as wells as cows in the early lactation period with low somatic cell counts [76]. Escherichia coli (E. coli) is a gram-negative environmental pathogen and is positive for catalase test and negative for coagulase test [77,78]. Many animals are the carriers, but cattle are the main carriers of E. coli.…”

Section: Escherichia Colimentioning

confidence: 99%

Etiology of Bovine Mastitis

Shoaib¹,

Aqib²,

Naseer³

et al. 2022

Mastitis in Dairy Cattle, Sheep and Goats

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Mastitis in dairy animals is the primary concern of dairy farmers, which is the most common disease that causes huge economic losses in the dairy industry. The economic losses due to mastitis are from a reduction in milk yield, condemnation of milk with antibiotic residues, veterinary treatment costs, and death. In addition, some mastitis pathogens also cause serious human diseases associated with the contamination of milk or milk products with bacteria or their toxins. Bovine mastitis is mainly caused by a wide range of environmental and contagious bacterial mastitis pathogens. Contagious pathogens are those whose main reservoir is the infected udder. Contagious pathogens mainly spread among animals during milking process whereas environmental pathogens spread from environment to udder at any time. The source of the environmental pathogens is the surrounding environment of an animal. The major contagious pathogens include Staphylococcus aureus, Streptococcus agalactiae, and Mycoplasma spp. and the minor contagious pathogens include Corynebacterium bovis and others. Major environmental pathogens include coliform bacteria (Escherichia coli, Klebsiella spp., Enterobacter spp. and Citrobacter spp.), environmental streptococci (Strep. dysgalactiae, Strep. uberis). This chapter covers detailed review of published data on contagious and environmental pathogens responsible for bovine mastitis.

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Recycling of Hazelnut Husk; from Bio‐waste to Phyto‐Assisted Synthesis of Silver Nanoparticles

Sivri,

Kurel,

Baytore

et al. 2023

ChemistrySelect

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In the present work, green synthesis is utilized in the synthesis of silver nanoparticles with hazelnut (Corylus Colurna) husk which is the outer leaf part of the hazelnut shell and is considered bio‐waste. According to transmission electron microscopy and x‐ray diffraction analysis, the morphology of the silver nanoparticles synthesized by 0.1 g/mL hazelnut husk extract is found to be spherical with an average diameter of 6.57 nm and possesses ultra‐narrow size distribution. UV‐visible spectrometry reveals the absorbance peak range between 450–475 nm which is in the range of surface plasmon resonance peak of silver nanoparticles with spherical morphology. Antibacterial properties of the synthesized silver nanoparticles were tested on E. coli and significant antimicrobial activity was found with up to 79 % areal inhibition efficiency. The research revealed that the hazelnut husk extract, a reducing agent used in syntheses with different mass concentrations of hazelnut husk extract, also affects the size of the nanoparticles, allowing for the possibility of controlling their size. The antibacterial properties of silver nanoparticles, synthesized in a spherical form of different sizes, correspondingly increased their effect on bacteria which is observed in the inhibition zone. The synthesis of silver nanoparticles, which can be used in many fields for agriculture, cosmetics, and medical purposes, using plant extract and minimal chemicals is crucial because it affects its toxicity. The synthesis of silver nanoparticles with hazelnut husk not only reduces the chemical waste and toxicity, but it is both easily accessible and helps the environment and sustainability by recycling a normally considered bio‐waste by‐product of high‐value‐added crop to a technologically valuable product silver. Moreover, synthesized silver nanoparticles could be an essential part of applications ranging from antibacterial surface treatments to drug delivery systems.

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Antimicrobial resistance modulation of MDR E. coli by antibiotic coated ZnO nanoparticles

Cited by 19 publications

References 34 publications

Evidence-Based Tracking of MDR E. coli from Bovine Endometritis and Its Elimination by Effective Novel Therapeutics

Evidence-Based Tracking of MDR E. coli from Bovine Endometritis and Its Elimination by Effective Novel Therapeutics

Etiology of Bovine Mastitis

Recycling of Hazelnut Husk; from Bio‐waste to Phyto‐Assisted Synthesis of Silver Nanoparticles

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