Antimicrobial resistance patterns and characterization of integrons of Shigella sonnei isolates in Seoul, 1999–2008

Jin, Young‐Hee; Oh, Youjin; Jung, Ji-Hun; Kim, Soo Jin; Kim, Jinah; Han, Ki-Young; Kim, Min-young; Park, Seog-gee; Lee, Young-Ki

doi:10.1007/s12275-010-9220-z

Cited by 14 publications

(8 citation statements)

References 24 publications

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“…Most Shigella strains harbored one or two classes of integrons. The presence of integrons in Shigella species varied in numbers in diverse geographical regions [1], [4], [26], [47], [48]. The apparent correlation between multidrug resistance and presence of integron in the strains was not due to the presence of integrons on conjugative plasmids.…”

Section: Discussionmentioning

confidence: 99%

Changing Trends in the Prevalence of Shigella Species: Emergence of Multi-Drug Resistant Shigella sonnei Biotype g in Bangladesh

et al. 2013

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Shigellosis, caused by Shigella species, is a major public health problem in Bangladesh. To determine the prevalence and distribution of different Shigella species, we analyzed 10,827 Shigella isolates from patients between 2001 and 2011. S. flexneri was the predominant species isolated throughout the period. However, the prevalence of S. flexneri decreased from 65.7% in 2001 to 47% in 2011, whereas the prevalence of S. sonnei increased from 7.2% in 2001 to 25% in 2011. S. boydii and S. dysenteriae accounted for 17.3% and 7.7% of the isolates respectively throughout the period. Of 200 randomly selected S. sonnei isolates for extensive characterization, biotype g strains were predominant (95%) followed by biotype a (5%). Resistance to commonly used antibiotics including trimethoprim-sulfamethoxazole, nalidixic acid, ciprofloxacin, mecillinam and ampicillin was 89.5%, 86.5%, 17%, 10.5%, and 9.5%, respectively. All isolates were susceptible to ceftriaxone, cefotaxime, ceftazidime and imipenem. Ninety-eight percent of the strains had integrons belonging to class 1, 2 or both. The class 1 integron contained only dfrA5 gene, whereas among class 2 integron, 16% contained dhfrAI-sat1-aadA1-orfX gene cassettes and 84% harbored dhfrA1-sat2 gene cassettes. Plasmids of ∼5, ∼1.8 and ∼1.4 MDa in size were found in 92% of the strains, whereas only 33% of the strains carried the 120 MDa plasmid. PFGE analysis showed that strains having different integron patterns belonged to different clusters. These results show a changing trend in the prevalence of Shigella species with the emergence of multidrug resistant S. sonnei. Although S. flexneri continues to be the predominant species albeit with reduced prevalence, S. sonnei has emerged as the second most prevalent species replacing the earlier dominance by S. boydii and S. dysenteriae in Bangladesh.

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Section: Discussionmentioning

confidence: 99%

Changing Trends in the Prevalence of Shigella Species: Emergence of Multi-Drug Resistant Shigella sonnei Biotype g in Bangladesh

et al. 2013

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“…The dentrogram of all the 76 isolates showed a 65% coefficient of similarity, which shared similar discriminatory power with S . sonnei isolates from other provinces [ 29 , 30 ] and other countries [ 3 , 31 ]. Isolates from close years were almost clustered closely and further divided into sub-clusters based on the region of isolation ( Fig 1 ), indicating the genetic diversity and the genetic changes over the timescales.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Shigella sonnei: An Increasingly Prevalent Etiologic Agent of Shigellosis in Guizhou Province, Southwest of China

Wang²,

Wei

et al. 2016

PLoS ONE

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BackgroundShigellosis is a serious problem in Guizhou and Shigella sonnei is an increasingly prevalent etiologic agent of local shigellosis cases. No data, however, are available about the molecular characterization of the local isolates of S. sonnei. We have conducted this study to molecularly characterize the clinical isolates of S. sonnei in Guizhou Province.Results76 S. sonnei isolates, including four isolates from 1974–1982 and 72 isolates from 2008–2010, were used for analysis in this study. Pulsed-field gel electrophoresis (PFGE) based on XbaI digestion divided the 76 isolates into 38 PFGE patterns (PT) and 15 PTs were represented by more than one isolates with PT31 (N = 8) containing the most number of isolates, followed with PT2 (N = 6). Multiple-Locus Variable number tandem repeat (VNTR) Analysis (MLVA) based on seven VNTR loci discriminated them into 19 different MLVA types (MTs), and four MTs were represented by more than one isolate with MT4 (N = 39) containing the most number of isolates, followed with MT12 (N = 18). 15 Multilocus sequence typing (MLST) base on 15 loci differentiated the isolates into six sequence types (STs), among which four STs were novel. The most common STs are ST76 (N = 43) and ST116 (N = 25), accounting for 92.1%. Correlation between genetic relationships and geographical origins or isolation years was observed among the isolates studied. Majority of isolates were clustered in accordance with the origin of isolation years based on the genetic data, which were also from similar geographical origins.ConclusionsOur results revealed the molecular characteristics including the specific genotypes such as four novel STs, clonal relationship, and genetic changes of local isolates from different years, which enhances our understanding of molecular characteristics of S. sonnei and contributes to the prevention and control of shigellosis in Guizhou Province.

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“…se ha incrementado en los últimos años como viene reportándose en diferentes regiones del mundo (8)(9)(10)(11)26), y se asocia principalmente a mecanismos de resistencia relacionados con la presencia de plásmidos, integrones de clase 1-2 y betalactamasas (27)(28)(29)(30).…”

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Caracterización molecular de aislamientos de Shigella sonnei recuperados en el Programa de Vigilancia por el Laboratorio de la Enfermedad Diarreica Aguda en Colombia

et al. 2015

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Realpe: identificación bioquímica y serotipificación María Elena Realpe: coordinación de las actividades del programa de vigilancia por el laboratorio de la enfermedad diarreica aguda del Grupo de Microbiología Todos los autores participaron en el análisis de resultados y la revisión del manuscrito. Caracterización molecular de aislamientos de Molecular characterization of Shigella sonnei isolates recovered by the Laboratory Surveillance Program for Acute Diarrheal Disease in ColombiaIntroduction: In Colombia, Shigella sonnei is one of the most frequently isolated serotypes (53.4%) in human clinical samples associated with diarrheal acute disease. The identification of DNA restriction patterns by pulsed field gel electrophoresis is the basis for the molecular surveillance of S. sonnei. Objective: To establish the basis for the molecular surveillance of S. sonnei in Colombia using pulsedfield gel electrophoresis. Materials and methods:We studied 102 of 2,048 S. sonnei isolates referred by the National Laboratory Network between 1997 and March, 2013; the selection was made according to the antimicrobial multiresistance profile, the source of samples, and the relation to outbreaks. The genetic profile was determined by pulsed field gel electrophoresis using the restriction enzymes XbaI and BlnI in accordance with the PulseNet International protocol. The electrophoretic patterns were analyzed with the GelCompare II, version 4.0 software. Results:We obtained 42 electrophoretic patterns with a 70% to 100% similarity. The most frequent pattern was COIN08J16X01.0017 with 17.6%, followed by patterns COIN04J16X01.0004 with

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Antimicrobial resistance patterns and characterization of integrons of Shigella sonnei isolates in Seoul, 1999–2008

Cited by 14 publications

References 24 publications

Changing Trends in the Prevalence of Shigella Species: Emergence of Multi-Drug Resistant Shigella sonnei Biotype g in Bangladesh

Changing Trends in the Prevalence of Shigella Species: Emergence of Multi-Drug Resistant Shigella sonnei Biotype g in Bangladesh

Molecular Characterization of Shigella sonnei: An Increasingly Prevalent Etiologic Agent of Shigellosis in Guizhou Province, Southwest of China

Caracterización molecular de aislamientos de Shigella sonnei recuperados en el Programa de Vigilancia por el Laboratorio de la Enfermedad Diarreica Aguda en Colombia

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