Antimicrobial Resistance Profile and Molecular Characterization of Extended-spectrum Beta-lactamase Genes in Enterobacteria Isolated from Human, Animal and Environment

Abstract

Objective: The aim of this study is to determine the antibiotic resistance profile and characterize extended spectrum beta-lactamase gene of enterobacteria strains isolated from human biological products, fecal matter of animals and the environment. Materials and Methods: Enterobacteria producing ESBL strains were isolated from human products, fecal matter of healthy animals (cattle, sheep and pigs) intended for human consumption and environment (hospital effluents and municipal sewage) using homemade medium (Drigalski supplemented with 2 mg/L of ceftazidime). Resistance to beta-lactams has been evaluated by the diffusion method was carried out as recommended by NCCLS. Characterization of Beta-Lactamase resistance genes (blaCTXM, blaSHV, blaTEM, blaGES, blaPER and blaVEB) was performed by simplex and multiplex PCR. Results: The strains were resistant to antibiotics from beta-lactam family (penicillin with inhibitor, monobactam, cephalosporin) but no resistant was observed to carbapenem (imipénème, méropénème). All resistance genes were identified in environment strains. Conclusion: This study showed the presence of common beta-lactam resistance genes (blaTEM, blaSHV and blaCTX-M) to human, animal and environment. The risk of dissemination and circulation of ESBL enterobacteria between animals, humans and the environment exists in Ivory Coast because of the absence of a barrier between them.

Intense Intestinal Carriage of Carbapenemase-Producing Klebsiella pneumoniae Co-harboring OXA-48, KPC, VIM, and NDM Among Preterm Neonates in a Moroccan Neonatal Intensive Care Unit

“…Other studies have found that blaNDM is the primary CR-KP gene found in neonatal patients [17], whereas blaKPC has been found in adults and older children [23]. Our findings also confirmed blaNDM as the prevailing carbapenemase gene within CR-KP isolates, aligning with prior research [17,24], and other reports from China [25]. Additionally, we identified two CR-KP strains expressing the blaKPC gene found in our study.…”

Occurrence of antibiotic-resistant bacteria in household greywater discharged into the environment in Burkina Faso's Sahel region

“…In fact, our results show a prevalence of identified MDR isolates belonging to the genera Salmonella spp. (Carole et al 2018). In addition, recent work has shown the presence of these species in raw greywater discharged to the environment without treatment in Israel (Porob et al 2020) and Yemen (Noman et al 2022).…”

Combined Proteotranscriptomic-Based Strategy to Discover Novel Antimicrobial Peptides from Cone Snails

“…An analysis of 83 studies conducted in Africa showed a prevalence of carbapenemase-producing bacteria isolated in hospitals ranging from 3% to 67.7%, with a mortality rate of up to 67% [ 36 ]. Beta-lactamases-producing bacteria and quinolone-resistant bacteria have been detected in humans, but also animals, and the environment [ 37 , 38 ]. Up to 35% of isolates from animals and 93.2% from humans have shown resistance to quinolones [ 37 ].…”