Antimicrobial resistance profile and resistance genes of Vibrio species isolated from giant freshwater prawn (Macrobrachium rosenbergii) raised in Iran

Abstract

Because of raising of large-scale high density prawn aquaculture techniques, production of this prawn worldwide is facing a serious threat from fatal diseases caused by nodaviruses and bacteria, particularly from the Vibrio species. The development of antibiotic resistance by Vibrio represents a potential threat to human health by exchange of resistant genes to human pathogens through food chain. This study aimed to determine antibiotic resistance profile of Vibrio isolates from giant fresh water Prawns (Macrobrachium rosenbergii) raised in Iran and to detect some antibiotic resistance genes in the isolates. A total of fifty giant fresh water prawns were processed for isolation of Vibrio species during February 2015 to August 2015. Identification of Vibrio isolates was done following standard biochemical methods. Phenotypic resistance of the isolates as determined by agar dilution method while polymerase chain reaction (PCR) method was used to detect the presence of erm, tetS, strA and sul2 genes in the isolates. Out of 50 prawns, 31 (62%) isolates of Vibrio spp. were reported, of which 20 (40%) were identified as V. parahaemolyticus, 10 (20%) were V. vulnificus and 1 (2%) were V. cholera. Over 90% of the tested strains showed susceptibility to SXT, AZM and NIT. In addition, strA and tetS genes were detected in all isolated Vibrio species. StrA gene was identified in 6 V. parahaemolyticus strains and also ermB and sul2 genes were not present in the isolate of V. cholera. The occurrence of multidrug resistance strains in the environment could be an indication of excessive usage of antibiotics in agriculture and aquaculture fields. This study has shown that giant freshwater prawns raised in Iran habour multidrug resistant Vibrio species.

Prevalence, antibiogram and virulence characterization of Vibrio isolates from fish and shellfish in Egypt: a possible zoonotic hazard to humans

“…Antimicrobial‐resistance genes, particularly those linked to mobile genetic elements, can be directly transmitted to humans through contaminated food (Shakerian et al . 2018).…”

Prevalence, Antibiotic Resistance, and Genetic Diversities of Clostridium difficile in Meat Nuggets from Various Sources in Isfahan, Iran

“…Diameters of the inhibition zones were interpreted based on the CLSI guidelines for the disc difusion method. In this method, antibiotic resistance was measured using standard disks of such antibiotics as amoxicillin, ampicillin, ceftaroline, clindamycin, linezolid, meropenem, metronidazole, moxifoxacin, penicillin, pyracylene, tetracycline, and vancomycin on the Mueller-Hinton agar medium according to the relevant protocols [17,18]. Te diameter of the zone of inhibition was read and interpreted after 48 hours of anaerobic incubation at 37 °C.…”

Systematic Survey of Vibrio spp. and Salmonella spp. in Bivalve Shellfish in Apulia Region (Italy): Prevalence and Antimicrobial Resistance

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