Antimicrobial Resistance Screening in Chlamydia trachomatis by Optimized McCoy Cell Culture System and Direct qPCR-Based Monitoring of Chlamydial Growth

Meštrović, Tomislav; Virók, Dezső; Ljubin‐Sternak, Sunčanica; Raffai, Tímea; Burián, Katalin; Vraneš, Jasmina

doi:10.1007/978-1-4939-9694-0_5

Cited by 2 publications

(3 citation statements)

References 19 publications

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“…First, the presence of Sf1Ep cells is necessary to promote long-term survival and multiplication of T. pallidum. The spirochete multiplies extracellularly; thus, the inclusion of mammalian cells does not introduce a potential permeability barrier, as is the case for intracellular bacteria such as Chlamydia (32). Second, incubation for 7 days was used in the T. pallidum assay instead of the 16 to 20 h typically used in broth dilution testing for most bacteria.…”

Section: Discussionmentioning

confidence: 99%

“…Third, microscopic bacterial quantitation was utilized instead of visual inspection for turbidity or pellet formation, because T. pallidum must be at high concentrations (Ͼ10 9 per ml) to produce turbidity and does not achieve these levels during in vitro culture. Quantitation by microscopy is time-consuming but likely provides more accurate results than assessment of turbidity; future studies could use other quantitation procedures, such as quantitative PCR (32). Prolonged incubation for antimicrobial susceptibility assessment is also needed for other slow-growing bacteria, most notably Mycobacterium tuberculosis (33).…”

Section: Discussionmentioning

confidence: 99%

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In Vitro Susceptibility of Treponema pallidum subsp. pallidum to Doxycycline

Edmondson

Wormser

Norris

2020

Antimicrob Agents Chemother

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Doxycycline is regarded as an effective therapy for early syphilis, and there is also increasing interest in using doxycycline for prophylaxis of this infection. However, the minimum inhibitory concentration (MIC) of doxycycline for Treponema pallidum subsp. pallidum has not been reported previously. In this study, an in vitro culture system was utilized to determine that the MIC of doxycycline is 0.06 to 0.10 μg/ml for four strains of T. pallidum subsp. pallidum (Nichols, SS14, UW231B, and UW249B). The Nichols strain cultured in vitro with doxycycline was also tested for infectivity in rabbits, and the minimum bactericidal concentration (MBC) was found to be ≤ 0.1 μg/ml using this method. The low MIC and MBC values are consistent with the previously demonstrated clinical efficacy of doxycycline for the treatment of early syphilis. This study represents the first report of the in vitro susceptibility of T. pallidum to doxycycline, and the resulting information may be useful in the consideration of doxycycline for use in prevention of syphilis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

In Vitro Susceptibility of Treponema pallidum subsp. pallidum to Doxycycline

Edmondson

Wormser

Norris

2020

Antimicrob Agents Chemother

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“…For example, the gastric pathogen Helicobacter pylori can have different mutations in the 23S rRNA, gyrA and rpoB genes, which are responsible for resistance to clarithromycin, ciprofloxacin and rifampicin, respectively ( 50 ). The capacity of Chlamydia trachomatis to resist antibiotics such as azithromycin, tetracycline and fluoroquinolone has also been attributed to spontaneous mutations ( 52 ). Although this mutation rate is not even across the board, there are bacterial subpopulations with a significant tendency to acquire and accumulate spontaneous mutations, which is why they often present a greater number of mutation events compared with what is commonly observed ( 21 ).…”

Section: Molecular Mechanisms Of Multidrug Resistance Generationmentioning

confidence: 99%

Molecular mechanisms of multidrug resistance in clinically relevant enteropathogenic bacteria (Review)

et al. 2022

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Multidrug resistant (MDR) enteropathogenic bacteria are a growing problem within the clinical environment due to their acquired tolerance to a wide range of antibiotics, thus causing severe illnesses and a tremendous economic impact in the healthcare sector. Due to its difficult treatment, knowledge and understanding of the molecular mechanisms that confer this resistance are needed. The aim of the present review is to describe the mechanisms of antibiotic resistance from a genomic perspective observed in bacteria, including naturally acquired resistance. The present review also discusses common pharmacological and alternative treatments used in cases of infection caused by MDR bacteria, thus covering necessary information for the development of novel antimicrobials and adjuvant molecules inhibiting bacterial proliferation. Contents1. Introduction 2. Multidrug resistant enteropathogenic bacteria 3. Molecular mechanisms of multidrug resistance 4. Molecular mechanisms of multidrug resistance generation 5. Drugs and bacterial response 6. Mechanisms of antibiotics 7. Mechanisms of drug resistance 8. Treatment against multidrug-resistant bacteria 9. Conclusions

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Antimicrobial Resistance Screening in Chlamydia trachomatis by Optimized McCoy Cell Culture System and Direct qPCR-Based Monitoring of Chlamydial Growth

Cited by 2 publications

References 19 publications

In Vitro Susceptibility of Treponema pallidum subsp. pallidum to Doxycycline

In Vitro Susceptibility of Treponema pallidum subsp. pallidum to Doxycycline

Molecular mechanisms of multidrug resistance in clinically relevant enteropathogenic bacteria (Review)

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