Antimicrobial Resistant Enterococcus faecium , Enterococcus faecalis , and Other Enterococcus Species Isolated From Foal Feces in Japan

Sukmawinata, Eddy; Sato, Wataru; Uemura, Ryoko; Sueyoshi, Masuo

doi:10.1016/j.jevs.2018.01.005

Cited by 9 publications

(5 citation statements)

References 12 publications

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“…Isolate presented MDR, being sensitive only to ciprofloxacin and resistant to all tested antibiotics of the penicillin, aminoglycoside, macrolides and cephalosporin class, similar data those of Herdan et al, (2012) Kim et al, (2016) and Sukmawinata et al, (2018) who found only 1.1% ampicillin resistance and 100% susceptibility to ampicillin bacteria respectively.…”

Section: Resultssupporting

confidence: 76%

Microbiological, serological and molecular diagnosis of abscess of withers in equine in the State of Pará

Rodrigues

Cruz

Barra

et al. 2021

RSD

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There are several infectious diseases that affect equines and can cause large economic losses in this sector and damage to animal welfare. In this species, abscessing lesions of a purulent character preferentially in the withers are strongly suggestive of brucellosis. However, few studies have been carried out on the disease in this species, making its identification and control difficult due to the lack of standardization in the interpretation of results. Therefore, samples of whole blood, serum and purulent secretion were collected from three horses. The animals came from the state of Pará. The diagnostic methods selected were the Rose-Bengal plate test (RBPT) and Polymerase Chain Reaction (PCR), for the negative molecular results for brucellosis, microbiological analysis were chosen. Although part of the samples were positive for brucellosis in serological tests, all of them showed negative results for PCR. In bacterial cultivation, species were isolated Streptococcus equi subsp. zooepidemicus, Enterococcus faecalis e Staphylococcus sciuri subsp. lentus., which showed resistance to antibacterials, especially the E. faecalis isolate classified as MDR (multi drug resistant). In conclusion, the best form of diagnosis is the joint evaluation of several tests, both screening and confirmatory, in order to obtain a definitive diagnosis with assertiveness.

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Section: Resultssupporting

confidence: 76%

Microbiological, serological and molecular diagnosis of abscess of withers in equine in the State of Pará

Rodrigues

Cruz

Barra

et al. 2021

RSD

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“…ESBL-producing E. coli (ESBLEC) have been reported in uterine, ocular, soft-tissue, colic, and wound infections in horses [20, 34]. Some studies have reported AMR in horses in Japan [19, 25, 32, 37]. However, there are no previous reports of ESBLEC isolated from horses in Japan.…”

mentioning

confidence: 99%

Extended-spectrum β-lactamase-producing <i>Escherichia coli</i> isolated from healthy Thoroughbred racehorses in Japan

Sukmawinata

Sato

Mitoma

et al. 2019

JES

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Extended-spectrum β-lactamase-producing Escherichia coli (ESBLEC) have become a major health concern in both human and veterinary medicine. These bacteria could become a critical problem in equine medicine due to the limited number of antimicrobial drugs available. However, there are no previous reports of ESBLEC isolated from horses in Japan. The objectives of this study were to investigate the occurrence of ESBLEC isolated from feces in healthy Thoroughbred racehorses in Japan. Feces samples were collected from 147 healthy Thoroughbred racehorses by equine veterinarians at the Japan Racing Association (103 from Miho Training Center and 44 from Ritto Training Center) between March 2017 and April 2018. Samples were screened for ESBLECs using MacConkey agar supplemented with 1 µg/ml cefotaxime. Detection of ESBL genes was performed by PCR and confirmed by DNA sequencing. Horizontal transmission was demonstrated by conjugation assay. In this study, 24 ESBLECs were isolated from twelve horse feces samples (8.2%). All ESBLECs harbored bla CTX-M-2 , and both bla TEM-1 and bla CTX-M-2 were detected in nine isolates (37.5%). ESBLECs showed resistance to all β-lactam antibiotics (100%) tested, followed by trimethoprim (66.7%), streptomycin (62.5%), tetracycline (25.0%), and oxytetracycline (25.0%). Horizontal transmission was successfully demonstrated by conjugation assay in eight of 13 isolates, and bla CTX-M-2 was detected by PCR in all transconjugants. This study showed that racehorses in Japan are potential reservoirs of ESBLECs.

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“…Enterococci are commensal bacteria residing in the GI tracts of almost all land animals; however, they can cause severe infections in humans 27 and have become important nosocomial pathogens globally. In a recent study by Sukmawinata et al 28 on Enterococci isolate profiling, 16.4% of E. faecalis were detected in a yearly collection of 72 faecal samples from healthy Japanese foals. Moreover, they found that the detected E. faecalis carried antimicrobial resistance to various antibiotics, for example, oxytetracycline, kanamycin, gentamycin and chloramphenicol.…”

Section: Resultsmentioning

confidence: 95%

Molecular Identification of Infectious Enteropathogens in Faeces of Healthy Horses

Paruch

2022

Microbiol�Insights

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Zoogenic faecal contamination of the environment is one of the indices included in the evaluation of ecological threats, health hazards and adverse impacts on various ecosystems. The risks and environmental concerns are associated with the fact that faeces of wild and domesticated animals constitute the largest source of environmental loading of enteropathogens associated with transmission of zoonotic diseases (enteric zoonoses). Although sick animals are more likely to transmit pathogens, healthy ones can also be the carriers and defecate them into the environment. This is of particular importance given the close human-animal interactions and health effects resulting from human and ecological exposures to faecal hazards from companion and farm animals. We have therefore set out to investigate whether healthy equines can carry and defecate human infectious pathogens. For this purpose, we set up a pilot study to examine the faecal DNA of horses using culture-independent molecular diagnostics – fluorescent probe-based quantitative real-time PCR. Our results revealed that among a total of 23 horses, 6 were found to carry Campylobacter jejuni ( C. jejuni), and 5 had Salmonella enterica serovar Typhimurium ( S. Typhimurium). Moreover, Enterococcus faecalis ( E. faecalis) was found in 14 horses, while 19 were positive for Clostridium perfringens ( C. perfringens). Furthermore, the frequently reported protozoan parasites in livestock, Cryptosporidium parvum ( C. parvum) and Giardia lamblia ( G. lamblia), were discovered in 8 and 7 samples, respectively. This pilot study shed new light on the phenomenon of healthy horses carrying C. jejuni and other human-health-related enteropathogens.

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Antimicrobial Resistant Enterococcus faecium , Enterococcus faecalis , and Other Enterococcus Species Isolated From Foal Feces in Japan

Cited by 9 publications

References 12 publications

Microbiological, serological and molecular diagnosis of abscess of withers in equine in the State of Pará

Microbiological, serological and molecular diagnosis of abscess of withers in equine in the State of Pará

Extended-spectrum β-lactamase-producing <i>Escherichia coli</i> isolated from healthy Thoroughbred racehorses in Japan

Molecular Identification of Infectious Enteropathogens in Faeces of Healthy Horses

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