Antimicrobial Screening of Endophytic Fungi Isolated from the Aerial Parts of Paepalanthus chiquitensis (Eriocaulaceae) Led to the Isolation of Secondary Metabolites Produced by Fusarium fujikuroi

Hilário, Felipe; Chapla, Vanessa Mara; Araújo, Ângela R.; Sano, Paulo Takeo; Bauab, Taís Maria; Santos, Lourdes Campaner dos

doi:10.21577/0103-5053.20160312

Cited by 11 publications

(9 citation statements)

References 15 publications

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“…This chemical study yielded an alkaloid 2-(4-butylpicolinamide) acetic acid ( 171 ) and three known compounds: fusaric acid ( 172 ), indole acetic acid ( 173 ), and terpestacin ( 174 ). The minimal inhibitory concentration of the extract, fusaric acid, and indole acetic acid had values from 125 to 1000 μg mL −1 [ 93 ].…”

Section: Resultsmentioning

confidence: 99%

Review on Compounds Isolated from Eriocaulaceae Family and Evaluation of Biological Activities by Machine Learning

et al. 2022

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Eriocaulaceae is a pantropical family whose main center of biodiversity is in Brazil. In general, the family has about 1200 species, in which phytochemical and biological studies have shown a variety of structures and activities. The aim of this research is to compile the compounds isolated in the Eriocaulaceae family and carry out a computational study on their biological targets. The bibliographic research was carried out on six databases. Tables were built and organized according to the chemical class. In addition, a summary of the methods of isolating the compounds was also made. In the computational study were used ChEMBL platform, DRAGON 7.0, and the KNIME 4.4.0 software. Two hundred and twenty-two different compounds have been isolated in sixty-eight species, divided mainly into flavonoids and naphthopyranones, and minor compounds. The ligand-based virtual screening found promising molecules and molecules with multitarget potential, such as xanthones 194, 196, 200 and saponin 202, with xanthone 194 as the most promising. Several compounds with biological activities were isolated in the family, but the chemical profiles of many species are still unknown. The selected structures are a starting point for further studies to develop new antiparasitic and antiviral compounds based on natural products.

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Section: Resultsmentioning

confidence: 99%

Review on Compounds Isolated from Eriocaulaceae Family and Evaluation of Biological Activities by Machine Learning

et al. 2022

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“…To date, only 2 studies have been reported in the literature involving the evaluation of secondary metabolites from strains of endophytic fungi isolated from Paepalanthus sp. (Eriocaulaceae) and which have exhibited antimicrobial activity potential [5,6].Paraphaeosphaeria sp. (Ascomycota: Montagnulaceae) are ubiquitous fungi that are present in soil as well as in various plants in different habitats [7].…”

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New Benzaldehyde and Benzopyran Compounds from the Endophytic Fungus Paraphaeosphaeria sp. F03 and Their Antimicrobial and Cytotoxic Activities

et al. 2019

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Three new benzaldehyde derivatives, sporulosaldeins A – C (1–3), and 3 new benzopyran derivatives, sporulosaldeins D – F (4–6), were discovered from an endophytic fungus, Paraphaeosphaeria sp. F03, which was isolated from Paepalanthus planifolius leaves. Compounds 1–6 were elucidated by 1- and 2-dimensional nuclear magnetic resonance experiments and high-resolution mass spectrometry analysis. The absolute configuration of compound 5 was determined through the comparison of experimental and calculated electronic circular dichroism data. Compounds 1–6 were found to exhibit antifungal activity with minimum inhibitory concentration (MIC) values of 7.8 – 250 µg/mL and racemic mixture of compound 6 exhibited weak cytotoxicity against MCF-7 and LM3 with IC50 values of 34.4 and 39.2 µM, respectively.

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“…In another aspect, F. fujikuroi is an industrial fungi used for the production of gibberellic acids, a group of extensive phytohormones (Cen et al, 2020). Extraction of secondary metabolites of F. fujikuroi led to the identification of alkaloid 2-(4-butylpicolinamide) acetic acid, terpestacin, and fusaric acid, among them only fusaric acid was moderately active against S. aureus, E. coli, and Salmonella setubal (Hilário et al, 2016). Given that 45 putative gene clusters were reported in F. fujikuroi using wholegenome sequencing (Wiemann et al, 2013), the possibility of exploiting new secondary metabolites with antibacterial and anticancer activity is still promising.…”

Section: Identification Of the Bioactive Strain Wqf5mentioning

confidence: 99%

\(\textit{Fusarium fujikuroi}\) WQF5 isolated from \(\textit{Cephalotaxus mannii }\)Hook.f. as a producer of antibacterial agent and paclitaxel

Tung

Phuong

et al. 2022

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Cephalotaxus mannii Hook.f is a rare medicinal plant used for the treatment of leukemia, however, its fungal endophytes with antibacterial and anticancer properties have not been exploited yet. In this study, six fungal isolates were recovered from roots of C. mannii collected in Ha Giang province, Vietnam. Among them, ethyl acetate crude extract of strain WQF5 exhibited strong antibacterial activity against 5 tested pathogens with inhibition zones ranging from 13.0 ± 0.5 mm to 20.0 ± 0.4 mm. Sulforhodamine B assay showed that WQF5 extract possessed the most potent cytotoxic activity against lung cancer A549 and breast cancer MCF7 cell lines with IC50 values of 6.9 ± 0.7 µg/mL and 23.1 ± 1.9 µg/mL, respectively. PCR-based molecular marker screening revealed that the positive hits for essential genes encoding 10-deacetylbaccatin III-10-O-acetyltransferase (dbat) and C-13 phenylpropanoyl side chain-CoA acyltransferase (bapt) involved in paclitaxel production were found in the fungal isolate WQF5. In addition, isolate WQF5, identified as Fusarium fujikuroi by morphological and ITS analysis, also produced paclitaxel as shown by HPLC-DAD analysis. This is the first report of bioprospecting endophytic fungi isolated from C. mannii, in which the capability of producing paclitaxel of endophytic fungi was also proved for the first time. These findings addressed a potent candidate for paclitaxel production and provided excellent material for further investigations of how endophytic fungi from non-Taxus plant species synthesize paclitaxel.

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Antimicrobial Screening of Endophytic Fungi Isolated from the Aerial Parts of Paepalanthus chiquitensis (Eriocaulaceae) Led to the Isolation of Secondary Metabolites Produced by Fusarium fujikuroi

Cited by 11 publications

References 15 publications

Review on Compounds Isolated from Eriocaulaceae Family and Evaluation of Biological Activities by Machine Learning

Review on Compounds Isolated from Eriocaulaceae Family and Evaluation of Biological Activities by Machine Learning

New Benzaldehyde and Benzopyran Compounds from the Endophytic Fungus Paraphaeosphaeria sp. F03 and Their Antimicrobial and Cytotoxic Activities

\(\textit{Fusarium fujikuroi}\) WQF5 isolated from \(\textit{Cephalotaxus mannii }\)Hook.f. as a producer of antibacterial agent and paclitaxel

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