Antimicrobial susceptibilities of Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis strains from periodontitis patients in Morocco

Mínguez, María José Aparicio; Ennibi, Oum Keltoum; Perdiguero, Pedro; Lakhdar, Leila; Abdellaoui, Lamia; Sanchez, Maria Carmen Arroyo; Sanz, Mariano; Herrera, David

doi:10.1007/s00784-018-2539-2

Cited by 18 publications

(32 citation statements)

References 34 publications

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“…In accordance, the present study showed that, in enrolled patients, the number of teeth and the levels of PD, CAL and BOP were significantly dependent on serum A. actinomycetemcomitans IgG antibody levels ( p < 0.001 for all observations). These results were in agreement with previous pivotal observations which highlighted a possible relationship between PT and high A. actinomycetemcomitans levels in gingival biofilm [ 38 ].…”

Section: Discussionsupporting

confidence: 93%

Association among serum and salivary A. actinomycetemcomitans specific immunoglobulin antibodies and periodontitis

et al. 2020

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Background The aim of this study was to assess the association between serum and salivary Immunoglobulin (Ig) Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) specific antibodies in healthy controls (HC) and periodontitis (PT) patients. Furthermore, the objectives were to determine whether PT influenced serum A. actinomycetemcomitans specific antibodies and whether serum or salivary antibodies against A. actinomycetemcomitans IgG were mediated by serum high-sensitivity c-reactive protein (hs-CRP). Methods Fifty-three patients with periodontitis and 48 HC were enrolled in the present study. Patients were regularly examined and characterized by clinical, salivary and blood samples analyses. A. actinomycetemcomitans IgA and IgG antibodies and hs-CRP were evaluated using a commercially available kit. The Spearman Correlation Test and Jonckheere-Terpstra Test were applied in order to assess the interdependence between serum A. actinomycetemcomitans IgG antibodies and clinical periodontal parameters. To evaluate the dependence of the serum and salivary A. actinomycetemcomitans IgG levels from possible confounders, univariate and multivariable linear regression analyses were performed. Results Compared to HC, patients with PT had significantly higher IgA [serum: PT, 1.89 (1.2–2.2) EU vs HC, 1.37 (0.9–1.8) EU (p = 0.022); saliva: PT, 1.67 (1.4–2.1) EU vs HC, 1.42 (0.9–1.6) EU (p = 0.019)] and A. actinomycetemcomitans IgG levels [serum: PT, 2.96 (2.1–3.7) EU vs HC, 2.18 (1.8–2.1) EU (p < 0.001); saliva, PT, 2.19 (1.8–2.5) EU vs HC, 1.84 (1.4–2) EU (p = 0.028)]. In PT patients, serum A. actinomycetemcomitans IgG were associated with a proportional extent of PT and tooth loss (P-trend value< 0.001). The univariate regression analysis demonstrated that PT (p = 0.013) and high hs-CRP (p < 0.001) had a significant negative effect on serum and salivary A. actinomycetemcomitans IgG levels. The multivariate regression analysis showed that PT (p = 0.033), hs-CRP (p = 0.014) and BMI (p = 0.017) were significant negative predictors of serum A. actinomycetemcomitans IgG while hs-CRP (p < 0.001) and BMI (P = 0.025) were significant negative predictors of salivary A. actinomycetemcomitans IgG. Conclusions PT patients presented a significantly higher serum and salivary A. actinomycetemcomitans IgA and IgG compared to HC. There was a significant increase in serum A. actinomycetemcomitans IgG when patients presented a progressive extent of PT. Moreover, PT and hs-CRP were significant negative predictors of increased salivary and serum A. actinomycetemcomitans IgG levels. Trial registration The study was retrospectively registered at clinicaltrials.gov (NCT04417322).

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Section: Discussionsupporting

confidence: 93%

Association among serum and salivary A. actinomycetemcomitans specific immunoglobulin antibodies and periodontitis

et al. 2020

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“…P. micros were further detected in about half of the patients ( n = 4507) but its prevalence varied annually ranging from 21.9 to 81.6%. Thus, annual prevalence mirrors the published highly variant ranges from 20.5% (Mínguez et al, 2018) to 100% (Rams et al, 2011).…”

Section: Discussionsupporting

confidence: 57%

“…Our study confirmed that subgingival plaque samples from periodontitis patients consistently contained species of the so‐called ‘Socransky‐complexes’ (Socransky & Haffajee, 2005). Comparison of our data with studies from the Netherlands and Spain (van Winkelhoff et al, 2005), the United States (Heller et al, 2012; Rams et al, 2013) or Morocco (Chahboun et al, 2015; Mínguez et al, 2018) revealed that the numbers of patients included in other studies were lower, for example, from 30 (van Winkelhoff et al, 2005) to 564 (Rams et al, 2013) (Table 4). Differences in the proportional representation of the periodontal species in different studies may, therefore, be explained by subject number, inclusion and exclusion criteria, local epidemiology and microbiological methodology.…”

Section: Discussionmentioning

confidence: 58%

Prevalence and antibiotic susceptibility trends of periodontal pathogens in the subgingival microbiota of German periodontitis patients: A retrospective surveillance study

Jepsen

Falk

Brune

et al. 2021

J Clinic Periodontology

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Objective This retrospective surveillance study aimed to follow periodontitis‐associated bacterial profiles and to identify time‐dependent changes in antibiotic susceptibility patterns. Materials and Methods From 2008 to 2015, bacterial specimen from deep periodontal pockets were collected from a total of 7804 German adults diagnosed with periodontitis. Presence of selected bacteria was confirmed by anaerobic culture and nucleic acid amplification. Antimicrobial susceptibility of clinical isolates was tested by disc diffusion with antibiotics used for the treatment of periodontitis and oral infections. The prevalences of periodontal pathogens were calculated and temporal evolution of antimicrobial susceptibility towards amoxicillin, amoxicillin/clavulanic acid, metronidazole, doxycycline, clindamycin, azithromycin, ciprofloxacin and ampicillin was analysed with logistic regression. Results The prevalence of patients harbouring bacteria was 95.9% Fusobacterium nucleatum, 88.0% Tannerella forsythia, 76.4% Treponema denticola, 76.5%, Campylobacter rectus, 76.0% Eikenella corrodens, 75.0% Capnocytophaga spp., 68.2% Porphyromonas gingivalis, 57.7% Peptostreptococcus micros, 43.1% Prevotella intermedia, 30.4% Eubacterium nodatum and 21.5% Aggregatibacter actinomycetemcomitans. In 63.5% of patients, one or more isolates were not susceptible to at least one of the antibiotics tested. The data further revealed a trend towards decreasing susceptibility profiles (p < 0.05) with antibiotic non‐susceptibilities in 37% of patients in 2008 and in 70% in 2015. Conclusions The present study confirmed a high prevalence of periodontal pathogens in the subgingival microbiota of German periodontitis patients. The data revealed an incremental increase in isolates displaying resistance to some antibiotics but no relevant change in susceptibility to amoxicillin and metronidazole.

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“…Thus, susceptibility testing of this antimicrobial is not relevant. Based on usage of breakpoint value for Haemophilus influenzae, most reports of susceptibility of A. actinomycetemcomitans to AML show that the prevalence of resistant isolates of the bacterium is low [35]. In addition, when isolates earlier found to be resistant to this antimicrobial were retested, the opposite result was achieved [36].…”

Section: Discussionmentioning

confidence: 99%

Chemical Composition, Antimicrobial activity, In Vitro Cytotoxicity and Leukotoxin Neutralization of Essential Oil from Origanum vulgare against Aggregatibacter actinomycetemcomitans

et al. 2020

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In this study, the essential oil of Origanum vulgare was evaluated for putative antibacterial activity against six clinical strains and five reference strains of Aggregatibacter actinomycetemcomitans, in comparison with some antimicrobials. The chemical composition of the essential oil was analyzed, using chromatography (CG) and gas chromatography–mass spectrometry coupled (CG–MS). The major compounds in the oil were Carvacrol (32.36%), α-terpineol (16.70%), p-cymene (16.24%), and Thymol (12.05%). The antimicrobial activity was determined by an agar well diffusion test. A broth microdilution method was used to study the minimal inhibitory concentration (MIC). The minimal bactericidal concentration (MBC) was also determined. The cytotoxicity of the essential oil (IC50) was <125 µg/mL for THP-1 cells, which was high in comparison with different MIC values for the A. actinomycetemcomitans strains. O. vulgare essential oil did not interfere with the neutralizing capacity of Psidium guajava against the A. actinomycetemcomitans leukotoxin. In addition, it was shown that the O. vulgare EO had an antibacterial effect against A. actinomycetemcomitans on a similar level as some tested antimicrobials. In view of these findings, we suggest that O.vulgare EO may be used as an adjuvant for prevention and treatment of periodontal diseases associated to A. actinomycetemcomitans. In addition, it can be used together with the previously tested leukotoxin neutralizing Psidium guajava.

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Antimicrobial susceptibilities of Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis strains from periodontitis patients in Morocco

Abstract: A. actinomycetemcomitans resistance against some antimicrobials in periodontitis patients in Morocco can influence the selection of the therapeutic approaches.

Cited by 18 publications

References 34 publications

Association among serum and salivary A. actinomycetemcomitans specific immunoglobulin antibodies and periodontitis

Association among serum and salivary A. actinomycetemcomitans specific immunoglobulin antibodies and periodontitis

Prevalence and antibiotic susceptibility trends of periodontal pathogens in the subgingival microbiota of German periodontitis patients: A retrospective surveillance study

Chemical Composition, Antimicrobial activity, In Vitro Cytotoxicity and Leukotoxin Neutralization of Essential Oil from Origanum vulgare against Aggregatibacter actinomycetemcomitans

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