Antimicrobial susceptibilities of Erysipelothrix rhusiopathiae

Venditti, Mario; Gelfusa, V.; Tarasi, A.; Brandimarte, C; Serra, Pietro

doi:10.1128/aac.34.10.2038

Cited by 76 publications

(40 citation statements)

References 15 publications

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“…It was possible to determine the MIC values for OTC in the present study, and sulfonamide and trimethoprim have been previously reported not to be effective against E. rhusiopathiae [9,11]. Despite the different media and methods employed in the present study for determination of the MICs in E. rhusiopathiae, the results for ABPC, OTC, EM, and LCM showed the same tendency as reported previously [7,9,11]. Although most of the strains were highly susceptible to fluoroquinolones, some of them were resistant.…”

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confidence: 91%

Antimicrobial Susceptibility of Erysipelothrix rhusiopathiae Isolated from Pigs in Southern Japan with a Modified Agar Dilution Method

Chuma

Kawamoto

Shahada

et al. 2010

The Journal of Veterinary Medical Science

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ABSTRACT. The determination of antimicrobial minimum inhibitory concentration (MIC) in Erysipelothrix rhusiopathiae by using the agar dilution method has not been covered by the Clinical and Laboratory Institute (CLSI). Only the broth microdilution method has been outlined. This report describes a modification of the agar dilution procedure for E. rhusiopathiae using Trypto-soy agar supplemented with 0.1% Tween 80 and incubation in ambient air at 37C for 24 hr. The MICs of the assay were in agreement with those of the broth microdilution method recommended by the CLSI. Antimicrobial susceptibility test was performed using this method for 149 E. Erysipelothrix rhusiopathiae is a Gram-positive bacillus and causative agent of swine erysipelas (SE). It causes great economic loss and continues to be a major problem in pig production [10]. The main clinical signs are septicemia and urticarial skin lesions in acute SE, milder signs are present in subacute SE and arthritis and endocarditis is occasionally present in chronic SE [5,10]. The bacteria can be isolated from mammals, fowl, fish, shellfish and even insects in most parts of world [4,10]. Human infection is usually related to occupational exposure of individuals, such as veterinarians, laboratory workers, and people handling meat, poultry and fish. There are three major categories of human disease: (i) the localized cutaneous form, erysipeloid; (ii) generalized cutaneous form; and (iii) septicemia, which is often associated with endocarditis [6,10]. Antibiotics, especially penicillins, have been used worldwide to treat SE and erysipelas in humans [4,10]. In Japan, about 2000 cases of swine erysipelas have been reported annually in recent years according to the National Institute of Animal Health, Japan. A live vaccine has been used for prevention and ampicillin and benzylpenicillin are generally used for treatment of SE [11]. The emergence of strains resistant to erythromycin and oxytetracycline was first described by Takahashi et al. [7]. However, there is a lack of reports of concerning antimicrobial susceptibility testing in the southern area of Japan where pigs are commonly raised.Currently, E. rhusiopathiae is referred to as fastidious bacterium by the Clinical and Laboratory Standards Institute [2]. Guidelines for the antimicrobial susceptibility test using broth dilution have been provided [2], however, guidelines for the agar dilution method were not covered.The aim of this study was to develop a modified agar dilution procedure specific for E. rhusiopathiae strains and to investigate the antimicrobial susceptibility of these strains towards commonly used classes of antimicrobials.One hundred and forty-nine E. rhusiopathiae strains were collected from internal iliac lymph nodes, arthritic fluid, urticaria, kidneys, spleens and knots of endocarditis of pigs at 2 meat processing plants located in Kagoshima Prefecture during the period of April 2004 to March 2005. The bacteria were isolated using Trypto-Soy agar (TS agar, Eiken Chemical Co., Ltd., Tokyo, Ja...

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confidence: 91%

Antimicrobial Susceptibility of Erysipelothrix rhusiopathiae Isolated from Pigs in Southern Japan with a Modified Agar Dilution Method

Chuma

Kawamoto

Shahada

et al. 2010

The Journal of Veterinary Medical Science

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“…Detection of the spaA gene in the present study suggests that this surface protective antigen may be a possible virulence factor for the above pathogenic effects of the isolate in cats. E. rhusiopathiae strains are known to vary considerably in virulence, and the surface protective antigen (Spa) has been indicated as one of the virulence factors to be involved in the pathogenicity of the disease [11,16]. To and Nagai further suggested that Spa proteins may be virulence factors specifically involved in the pathogenesis of E. rhusiopathiae, and from a practical point of view, the presence or absence of spa may be a suitable marker for the differentiation of E. rhusiopathiae and E. tonsillarum [15].…”

Section: Discussionmentioning

confidence: 99%

“…Antimicrobial susceptibility test: Antimicrobial susceptibility tests were determined by the disc diffusion method [16] using discs containing the following antibiotics and contents, which are indicated in parentheses: ampicillin ( were used as quality control. Zone diameter breakpoints [3] were used for interpretation of resistance to those agents.…”

Section: Sample Collection and Bacterial Culturementioning

confidence: 99%

“…The recommended method for serologic investigation of E. rhusiopathiae has been reported to be the double agar-gel diffusion precipitation test with type-specific rabbit antisera and a heat-stable antigen prepared by hot aqueous extraction [7]. In addition, the in vitro antimicrobial susceptibility profiles of the E. rhusiopathiae clinical isolates have been reported [13,16].…”

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confidence: 99%

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Characterization and Identification of Erysipelothrix rhusiopathiae Isolated from an Unnatural Host, a Cat, with a Clinical Manifestation of Depression

Lee

Kim

Lim

et al. 2011

The Journal of Veterinary Medical Science

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ABSTRACT. Erysipelothrix rhusiopathiae is pathogenic for humans, many domestic animals and wild birds, but infectious cases with clinical symptoms in cats have not been reported. E. rhusiopathiae was recovered from a 4-month Russian blue breed cat with a very poor body condition score of 1 (BCS: 1/5). The isolate was typed as serotype 2b. Mice experimentally infected with the clinical isolate of E. rhusiopathiae through subcutaneous or intraperitoneal routes survived, and the organism was recovered from the spleen and synovial and pericardial fluids. Cats experimentally inoculated with the isolate either orally or subcutaneously survived but commonly exhibited depression and emaciation together with localized erythemal lesion of the skin accompanied by purulent ocular discharge. On hematological analysis, the number of total white blood cells was high compared with that in normal cats. Histological examination revealed congestion and moderate inflammation with focal necrosis. This observation may provide insight on E. rhusiopathiae infection in cats with the possible epidemiological significance and implications as a potential source of infection to other animals and humans.

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“…El tratamiento en base a penicilina fue exitoso en el caso descrito. Daptomicina o las quinolonas son alternativas cuando los betalactámicos están contraindicados 13,14 . La duración del tratamiento intravenoso es de 4 a 6 semanas, si bien hay reportados tratamientos intravenosos más breves combinados con vía oral 10 .…”

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