Antimicrobial Susceptibilities of
 Vibrio parahaemolyticus
 and
 Vibrio vulnificus
 Isolates from Louisiana Gulf and Retail Raw Oysters

Han, Fenglin; Walker, Robert D.; Janes, Marlene E.; Prinyawiwatkul, Witoon; Ge, Beilei

doi:10.1128/aem.01116-07

Cited by 163 publications

(140 citation statements)

References 24 publications

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“…Resistance to ampicillin was prevalent, which was in agreement with the previous studies (Roque et al, 2001;Akinbowale et al, 2006). The result is also in agreement with other studies that reported V. parahaemolyticus isolated from seafood samples are commonly resistance to ampicillin (Okuda et al, 1997;Han et al, 2007;Al-Othrubi et al, 2014). The ampicillin-resistant pattern could be due to the fact that first generation antibiotics, including ampicillin, is misused in the environment thus reducing the susceptibility and efficiency of ampicillin in the treatment of Vibrio infection (Sudha et al, 2014).…”

Section: Discussionsupporting

confidence: 83%

Antimicrobial resistance profile and resistance genes of Vibrio species isolated from giant freshwater prawn (Macrobrachium rosenbergii) raised in Iran

Shakerian

Barton

Akinbowale

et al. 2018

J Hellenic Vet Med Soc

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Because of raising of large-scale high density prawn aquaculture techniques, production of this prawn worldwide is facing a serious threat from fatal diseases caused by nodaviruses and bacteria, particularly from the Vibrio species. The development of antibiotic resistance by Vibrio represents a potential threat to human health by exchange of resistant genes to human pathogens through food chain. This study aimed to determine antibiotic resistance profile of Vibrio isolates from giant fresh water Prawns (Macrobrachium rosenbergii) raised in Iran and to detect some antibiotic resistance genes in the isolates. A total of fifty giant fresh water prawns were processed for isolation of Vibrio species during February 2015 to August 2015. Identification of Vibrio isolates was done following standard biochemical methods. Phenotypic resistance of the isolates as determined by agar dilution method while polymerase chain reaction (PCR) method was used to detect the presence of erm, tetS, strA and sul2 genes in the isolates. Out of 50 prawns, 31 (62%) isolates of Vibrio spp. were reported, of which 20 (40%) were identified as V. parahaemolyticus, 10 (20%) were V. vulnificus and 1 (2%) were V. cholera. Over 90% of the tested strains showed susceptibility to SXT, AZM and NIT. In addition, strA and tetS genes were detected in all isolated Vibrio species. StrA gene was identified in 6 V. parahaemolyticus strains and also ermB and sul2 genes were not present in the isolate of V. cholera. The occurrence of multidrug resistance strains in the environment could be an indication of excessive usage of antibiotics in agriculture and aquaculture fields. This study has shown that giant freshwater prawns raised in Iran habour multidrug resistant Vibrio species.

show abstract

Section: Discussionsupporting

confidence: 83%

Antimicrobial resistance profile and resistance genes of Vibrio species isolated from giant freshwater prawn (Macrobrachium rosenbergii) raised in Iran

Shakerian

Barton

Akinbowale

et al. 2018

J Hellenic Vet Med Soc

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“…The isolate was not susceptible only to ampicillin and susceptible to the remaining 12 antibiotics. These results are consistent with those of previous studies (25,38,39), which indicated that many V. parahaemolyticus isolates were resistant to ampicillin and susceptible to most of the other examined antibiotics.…”

Section: Discussionsupporting

confidence: 83%

The First Report of Vibrio parahaemolyticus Strain O10:K60 in Japan, a New Combination of O and K Serotypes Isolated from a Patient with Gastroenteritis

et al. 2016

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SUMMARY:Vibrio parahaemolyticus is an important pathogen that causes gastroenteritis in humans, generally associated with the consumption of contaminated seafood, particularly raw shellfish. There are many serotypes in V. parahaemolyticus resulting from a combination of O and K antigens. Among them, O3:K6 and their variants, which represent the pandemic clone, are the most widespread strains worldwide. In this study, we examined V. parahaemolyticus isolated from a gastroenteritis patient's stool at a hospital in Saitama City, Japan in 2013. Serotyping of the O and K antigens identified the strain as O10:K60. To our knowledge, this is the first reported case of a V. parahaemolyticus strain with this antigen combination in Japan. Subsequently, we used PCR to assay for pathogenicity-associated genes, and found that it was positive for tdh, T3SS1, and T3SS2a genes. Antibiotic susceptibility tests showed that the strain was susceptible to all selected antibiotics except ampicillin. Moreover, we detected specific marker genes for the pandemic clone with two kinds of PCR assay. Our results suggest that the isolate O10:K60 is a newly emerging serotype that belongs to the pandemic clone.

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“…There are many different used antibiotics for treatment for Vibrio species infections (Han et al, 2007;Al-Othrubi et al, 2014). Our dependence on antibiotics to control bacterial infections in agriculture, aquaculture, veterinary medicine and humans resulted to indiscriminate use which in turn led to the emergence of multidrug resistant strains in the biosphere (Rao and Lalitha, 2015;(Letchumanan et al, 2015;Shrestha et al, 2015;Zavala Norzagaray et al, 2015).…”

Section: Issn: 2319-7706 Volume 6 Number 6 (2017) Pp 2760-2778mentioning

confidence: 99%

Phenotypic Characterization of Vibrio Species: Application of Indigenous Phages for Biological Control of Vibrio in Aquaculture Live Feeds

Hassan¹

2017

Int.J.Curr.Microbiol.App.Sci

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Antimicrobial Susceptibilities of Vibrio parahaemolyticus and Vibrio vulnificus Isolates from Louisiana Gulf and Retail Raw Oysters

Cited by 163 publications

References 24 publications

Antimicrobial resistance profile and resistance genes of Vibrio species isolated from giant freshwater prawn (Macrobrachium rosenbergii) raised in Iran

Antimicrobial resistance profile and resistance genes of Vibrio species isolated from giant freshwater prawn (Macrobrachium rosenbergii) raised in Iran

The First Report of <i>Vibrio parahaemolyticus</i> Strain O10:K60 in Japan, a New Combination of O and K Serotypes Isolated from a Patient with Gastroenteritis

Phenotypic Characterization of Vibrio Species: Application of Indigenous Phages for Biological Control of Vibrio in Aquaculture Live Feeds

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