Antimicrobial susceptibility and genetic characterization of Escherichia coli recovered from frozen game meat

Mateus-Vargas, Rafael H.; Atanassova, Viktoria; Reich, Felix; Klein, Günter

doi:10.1016/j.fm.2016.11.013

Cited by 16 publications

(12 citation statements)

References 36 publications

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“…To the authors' knowledge, the present study is the first to report the presence of ESβL/AmpC E. coli isolated directly from the fresh meat of wild boar. In fact, a recent paper published in 2017 [14] reported one E. coli harboring the bla CTX-M1 gene isolated from frozen red deer meat but none from wild boar meat.…”

Section: Discussionmentioning

confidence: 99%

“…The spread of AMR is considered an important public health risk and is related both to pathogenic and commensal microorganisms. For example, E. coli, an intestinal commensal microorganism, can sometimes cause community-onset infection even in healthy people [14] or can be a reservoir of resistance genes for pathogenic bacteria [15]. In the last few decades, studies have reported an increase in the prevalence of multidrug-resistant coliform bacteria, not only in hospital settings, but also in swine herds (Sus scrofa domesticus) [14], wild animals (particularly the wild boar, Sus scrofa) and in their meat products [16].…”

Section: Introductionmentioning

confidence: 99%

“…AMR bacteria can also be transmitted through the food chain and particularly through the consumption of meat products [14,23]. Meat contamination is largely related to the process of slaughtering and/or hunting.…”

Section: Introductionmentioning

confidence: 99%

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Prevalence of ESβL, AmpC and Colistin-Resistant E. coli in Meat: A Comparison between Pork and Wild Boar

et al. 2021

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A global increase in Escherichia coli (E. coli) resistant to cephalosporins (extended-spectrum β-lactamases (ESβLs) and AmpC β-lactamases) has been recorded in the last 20 years. Similarly, several studies have reported the spread of colistin resistance in Enterobacteriaceae isolated from food and the environment. The aim of the present study was to evaluate the prevalence of ESβL, AmpC and colistin-resistant E. coli isolated from pork and wild boar meat products in the Emilia Romagna region (North Italy). The isolates were analysed phenotypically (considering both resistant and intermediate profiles) and genotypically. The prevalence of genotypically confirmed ESβL and AmpC E. coli was higher in pork meat products (ESβL = 11.1% vs. AmpC = 0.3%) compared to wild boar meat (ESβL = 6.5% vs. AmpC = 0%). Intermediate profiles for cefotaxime (CTX) and ceftazidime (CAZ) were genotypically confirmed as ESβL in pork meat isolates but not for wild boar. Four E. coli from wild boar meat were resistant to colistin but did not harbour the mcr-1 gene. E. coli isolated from wild boar meat seem to show aspecific antimicrobial resistance mechanisms for cephalosporins and colistin. The prevalence of resistant isolates found in wild boar is less alarming than in pork from farmed domestic pigs. However, the potential risk to consumers of these meat products will require further investigations.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Prevalence of ESβL, AmpC and Colistin-Resistant E. coli in Meat: A Comparison between Pork and Wild Boar

et al. 2021

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“…The most frequently isolated serotypes were E. coli O26:H11 and E. coli O55:H7. Similarly, ETEC and EPEC serovars were isolated from imported meat, poultry, and game meat worldwide, including Malaysia (Abuelhassan et al, 2016), Germany (Mateus-Vargas et al, 2017), India (Hussain et al, 2017), Ghana (Eibach et al, 2018), and Peru (Ruiz-Roldán et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Multidrug Resistant Shiga Toxin-Producing Escherichia coli in Cattle Meat and Its Contact Surfaces

Darwish

Atia

El‐Ghareeb

et al. 2018

J. Food Qual. Hazards Control

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“…Some studies have detected MRSA or ESBL-producing E. coli in game meat [18][19][20] or the feces and tissues of wild animals such as deer, mice, various bird species [21,22], and wild boars [23,24]. This is of particular concern as the handling and consumption of contaminated game meat could constitute a transmission route for resistant bacteria from wild animals to humans.…”

Section: Introductionmentioning

confidence: 99%

Wild Boars Carry Extended-Spectrum β-Lactamase- and AmpC-Producing Escherichia coli

et al. 2021

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Extended-spectrum β-lactamase (ESBL)-producing Escherichia coli and methicillin-resistant Staphylococcus aureus (MRSA) represent major healthcare concerns. The role of wildlife in the epidemiology of these bacteria is unclear. The purpose of this study was to determine their prevalence in wild boars in Germany and to characterize individual isolates. A total of 375 fecal samples and 439 nasal swabs were screened for the presence of ESBL-/AmpC-E. coli and MRSA, respectively. The associations of seven demographic and anthropogenic variables with the occurrence of ESBL-/AmpC-E. coli were statistically evaluated. Collected isolates were subjected to antimicrobial susceptibility testing, molecular typing methods, and gene detection by PCR and genome sequencing. ESBL-/AmpC-E. coli were detected in 22 fecal samples (5.9%) whereas no MRSA were detected. The occurrence of ESBL-/AmpC-E. coli in wild boars was significantly and positively associated with human population density. Of the 22 E. coli, 19 were confirmed as ESBL-producers and carried genes belonging to blaCTX-M group 1 or blaSHV-12. The remaining three isolates carried the AmpC-β-lactamase gene blaCMY-2. Several isolates showed additional antimicrobial resistances. All four major phylogenetic groups were represented with group B1 being the most common. This study demonstrates that wild boars can serve as a reservoir for ESBL-/AmpC-producing and multidrug-resistant E. coli.

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Antimicrobial susceptibility and genetic characterization of Escherichia coli recovered from frozen game meat

Cited by 16 publications

References 36 publications

Prevalence of ESβL, AmpC and Colistin-Resistant E. coli in Meat: A Comparison between Pork and Wild Boar

Prevalence of ESβL, AmpC and Colistin-Resistant E. coli in Meat: A Comparison between Pork and Wild Boar

Prevalence of Multidrug Resistant Shiga Toxin-Producing Escherichia coli in Cattle Meat and Its Contact Surfaces

Wild Boars Carry Extended-Spectrum β-Lactamase- and AmpC-Producing Escherichia coli

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