Antimicrobial Susceptibility of <i>Helicobacter pylori</i> Strains in a Random Adult Swedish Population

Storskrubb, Tom; Aro, Pertti; Ronkainen, Jukka; Wreiber, Karin; Nyhlin, Henry; Bolling–Sternevald, Elisabeth; Talley, Nicholas J.; Engstrand, Lars; Agréus, Lars

doi:10.1111/j.1523-5378.2006.00414.x

Cited by 38 publications

(36 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Treatment with macrolides induces point mutations in the 23S rRNA gene that could result in the acquisition of resistance (41). Despite the heterogeneity of our population (33), mutations involved in CLR r were comparable to mutations reported previously by other studies in Europe, which were mainly A2142G/43G mutations and various combinations of the wild type as well as point mutations related to the presence of multiple isolates (mixed population) in the same biopsy specimen (1,12,31,37,43,44,49). In contrast with data from many previous studies (1,2,11,24), our study identified the A2142C mutation in some strains from children, probably due to the large number of isolates in our study.…”

Section: Vol 49 2011 Two-decade Survey Of H Pylori Resistance In Bmentioning

confidence: 50%

See 1 more Smart Citation

Multicenter Survey of Routine Determinations of Resistance of Helicobacter pylori to Antimicrobials over the Last 20 Years (1990 to 2009) in Belgium

et al. 2011

View full text Add to dashboard Cite

We analyzed the rates of antimicrobial resistance of Helicobacter pylori strains isolated from patients from 1990 to 2009 and identified risk factors associated with resistance. Gastric biopsy specimens were collected from several digestive disease centers in Brussels, Belgium. We routinely performed antimicrobial susceptibility testing for clarithromycin (CLR), metronidazole, amoxicillin, tetracycline, and ciprofloxacin. Evaluable susceptibility testing was obtained for 9,430 strains isolated from patients who were not previously treated for Helicobacter pylori infection (1,527 isolates from children and 7,903 from adults) and 1,371 strains from patients who were previously treated (162 isolates from children and 1,209 from adults). No resistance to amoxicillin was observed, and tetracycline resistance was very rare (<0.01%). Primary metronidazole resistance remained stable over the years, with significantly lower rates for isolates from children (23.4%) than for isolates from adults (30.6%). Ciprofloxacin resistance remained rare in children, while it increased significantly over the last years in adults. Primary clarithromycin resistance increased significantly, reaching peaks in 2000 for children (16.9%) and in 2003 for adults (23.7%). A subsequent decrease of resistance rates down to 10% in both groups corresponded to a parallel decrease in macrolide consumption during the same period. Multivariate logistic regression revealed that female gender, age of the patient of 40 to 64 years, ethnic background, the number of previously unsuccessful eradication attempts, and the different time periods studied were independent risk factors of resistance to clarithromycin, metronidazole, and ciprofloxacin. Our study highlights the need to update local epidemiological data. Thus, the empirical CLR-based triple therapy proposed by the Maastricht III consensus report remains currently applicable to our population.

show abstract

Section: Vol 49 2011 Two-decade Survey Of H Pylori Resistance In Bmentioning

confidence: 50%

“…As found for many other countries, H. pylori resistance to TET and AMX was very infrequent in our population (6,14,31,44,50). Therefore, our analysis focused on resistance to CLR, MET, and CIP.…”

Section: Discussionmentioning

confidence: 99%

Multicenter Survey of Routine Determinations of Resistance of Helicobacter pylori to Antimicrobials over the Last 20 Years (1990 to 2009) in Belgium

et al. 2011

View full text Add to dashboard Cite

show abstract

“…(23). Strains HPAG-KX1A1, HPAG-KX1C1, HPCa-KX2A2, and HPCa-KX2C1 were from the Kalixanda study (31,32). HPAG-KX1 strains were from an de-identified patient with ChAG (HPAG-KX1A1 from the antrum and HPAG-KX1C1 from the corpus).…”

Section: Resultsmentioning

confidence: 99%

The complete genome sequence of a chronic atrophic gastritis Helicobacter pylori strain: Evolution during disease progression

Oh¹,

Kling-Bäckhed

Giannakis

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

227

200

View full text Add to dashboard Cite

Helicobacter pylori produces acute superficial gastritis in nearly all of its human hosts. However, a subset of individuals develops chronic atrophic gastritis (ChAG), a condition characterized in part by diminished numbers of acid-producing parietal cells and increased risk for development of gastric adenocarcinoma. Previously, we used a gnotobiotic transgenic mouse model with an engineered ablation of parietal cells to show that loss of parietal cells provides an opportunity for a H. pylori isolate from a patient with ChAG (HPAG1) to bind to, enter, and persist within gastric stem cells. This finding raises the question of how ChAG influences H. pylori genome evolution, physiology, and tumorigenesis. Here we describe the 1,596,366-bp HPAG1 genome. Custom HPAG1 Affymetrix GeneChips, representing 99.6% of its predicted ORFs, were used for whole-genome genotyping of additional H. pylori ChAG isolates obtained from Swedish patients enrolled in a casecontrol study of gastric cancer, as well as ChAG-and cancerassociated isolates from an individual who progressed from ChAG to gastric adenocarcinoma. The results reveal a shared gene signature among ChAG strains, as well as genes that may have been lost or gained during progression to adenocarcinoma. Wholegenome transcriptional profiling of HPAG1's response to acid during in vitro growth indicates that genes encoding components of metal uptake and utilization pathways, outer membrane proteins, and virulence factors are among those associated with H. pylori's adaptation to ChAG. acid regulation ͉ comparative microbial genomics ͉ ecogenomics ͉ functional genomics ͉ gastric cancer

show abstract

“…Globally, the antibiotic resistance of H. pylori ranges from 10 to 80% for metronidazole, 1-25% for clarithromycin, and <1% for amoxicillin [7]. In Sweden, antibiotic resistance for H. pylori is lower than average, with an estimated 16% for metronidazole, 2% for clarithromycin and 0% for amoxicillin [8]. These numbers are based on studies performed more than a decade ago in Sweden and no recent data on antibiotic resistance in H. pylori is available.…”

Section: Introductionmentioning

confidence: 99%

Helicobacter pylori eradication in the Swedish population

Doorakkers

Lagergren

Gajulapuri

et al. 2017

Scandinavian Journal of Gastroenterology

Self Cite

View full text Add to dashboard Cite

Objectives: Helicobacter pylori is associated with peptic ulcers and gastric cancer and its eradication aims to prevent these conditions. The recommended eradication regimen is triple therapy, consisting of a proton-pump inhibitor in combination with clarithromycin and amoxicillin or metronidazole for 7 days. Yet, other antibiotic regimens are sometimes prescribed. We aimed to assess the use of eradication therapy for H. pylori in the Swedish population during the last decade. Materials and methods: This population-based study used data from the Swedish Prescribed Drug Register. From July 2005 until December 2014, all regimens that can eradicate H. pylori were identified and evaluated according to patients' age and sex and calendar year of eradication. Results: We identified 157,915 eradication episodes in 140,391 individuals (53.8% women, 42.6% older than 60 years), who correspond to 1.5% of the Swedish population. The absolute number and incidence of eradications decreased over the study period. Overall, 91.0% had one eradication and 0.1% had more than three. Of all eradications, 95.4% followed the recommended regimen, while 4.7% did not. The latter group was overrepresented among individuals aged 80 years (7.8%). Amoxicillin and clarithromycin were most frequently prescribed, while metronidazole was rarely used (0.01%). Other prescribed antibiotics were ciprofloxacin (2.4%), doxycycline (1.4%), nitrofurantoin (0.7%), norfloxacin (0.5%) and erythromycin (0.3%). Conclusions: During the last decade in Sweden H. pylori eradication has been frequently prescribed, but the incidence of eradication has slowly declined. Most eradications followed the recommended regimen, including those occurring after a previous eradication. ARTICLE HISTORY

show abstract

Antimicrobial Susceptibility of Helicobacter pylori Strains in a Random Adult Swedish Population

Cited by 38 publications

References 30 publications

Multicenter Survey of Routine Determinations of Resistance of Helicobacter pylori to Antimicrobials over the Last 20 Years (1990 to 2009) in Belgium

Multicenter Survey of Routine Determinations of Resistance of Helicobacter pylori to Antimicrobials over the Last 20 Years (1990 to 2009) in Belgium

The complete genome sequence of a chronic atrophic gastritis Helicobacter pylori strain: Evolution during disease progression

Helicobacter pylori eradication in the Swedish population

Contact Info

Product

Resources

About