Antimicrobial susceptibility of lactic acid bacteria isolated from human and food-producing animal feces in Khon Kaen Province, Thailand

Sornplang, Pairat; Sakulsawasdiphan, Kattinet; Piyadeatsoontorn, Sudthidol; Surasorn, Benyapha

doi:10.1007/s11250-016-1116-4

Cited by 11 publications

(7 citation statements)

References 17 publications

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“…Specifically for enterococci, multidrug resistance patterns found are in agreement to that reported for E. hirae , E. faecium , and E. durans from dairy cows feces, E. hirae being resistant up to seven antimicrobials ( Jackson et al, 2010 ). Nevertheless, L. fermentum CRL2085 from feedlot cattle was phenotypically sensitive to all assayed antibiotics in this study, in disagreement to that reported for fermented food and animal/human feces strains which were resistant to ERY and TET ( Ammor et al, 2007 ; Nawaz et al, 2011 ; Sornplang et al, 2016 ).…”

Section: Resultscontrasting

confidence: 99%

“…Moreover, an unexpected high prevalence of TET resistance was observed among feedlot Lactobacillus (78% of the strains) with MICs values far beyond the cut-off value (128 μg/ml). L. acidophilus CRL2152, L. amylovorus CRL2115 and 4 strains of L. mucosae exhibited the highest TET resistance level in agreement with those reported for food and animal feces lactobacilli ( Klose et al, 2014 ; Sornplang et al, 2016 ).…”

Section: Resultssupporting

confidence: 86%

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Safety and Growth Optimization of Lactic Acid Bacteria Isolated From Feedlot Cattle for Probiotic Formula Design

et al. 2018

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In order to eliminate the widespread use of antibiotics in livestock production, the research for alternatives has increased lately. This study examined the safety of 40 lactic acid bacteria (LAB) isolated from bovine feedlot environment and previously selected as potential probiotics. A high sensitivity prevalence to ampicillin (AMP, 100%), gentamicin (GEN, 96.3%), kanamycin (KAN, 96.3%), clindamycin (CLI, 85.2%), chloramphenicol (CHL, 92.6%) and streptomycin (STR, 88.9%) while moderate and high resistance against erythromycin (ERY, 48%) and tetracycline (TET, 79%) respectively, were determined. Feedlot enterococci and pediococci displayed high resistance to CLI, ERY, GEN and TET (73, 100, 54.5, and 73%, respectively). Among fifteen resistance genes investigated, seven were identified in lactobacilli; their presence not always was correlated with phenotypic resistance. STR resistance genes, aadA and ant(6) were observed in 7.4 and 3.7% of isolates, respectively; genes responsible for aminoglycosides resistance, such as bla (7.4%), and aph(3”)-III (3.7%) were also recognized. In addition, resistance cat and tetS genes (3.7 and 7.4%, respectively) were harbored by feedlot lactobacilli strains. The presence of ermB gene in 22.3% of isolates, including two of the six strains phenotypically resistant to ERY, exhibited the highest prevalence among the assessed antibiotics. None of the feedlot lactobacilli harbored virulence factors genes, while positive PCR amplification for ace, agg, fsrA, and atpA genes was found for enterococci. With the objective of producing large cell biomass for probiotic delivery, growth media without peptone but containing glucose and skim milk powder (Mgl and Mlac) were selected as optimal. Lactobacillus acidophilus CRL2074, L. amylovorus CRL2115, L. mucosae CRL2069, and L. rhamnosus CRL2084 were strains selected as free of antibiotic resistance and virulence determinants, able to reach high cell numbers in non-expensive culture media and being compatible among them.

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Section: Resultscontrasting

confidence: 99%

Section: Resultssupporting

confidence: 86%

Safety and Growth Optimization of Lactic Acid Bacteria Isolated From Feedlot Cattle for Probiotic Formula Design

et al. 2018

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“…To the best of our knowledge, this is the first report of prevalent antimicrobial resistance of Salmonella in laying hen and environmental samples in Thailand. This study's high Salmonella resistance rate to tetracycline may have been due to the frequent use or misuse of this drug in food-producing animals in Thailand [22,23]. In similar studies in Turkey [24,25] and Iran [26], Salmonella isolates were highly resistant to tetracycline in laying hens.…”

Section: Discussionmentioning

confidence: 68%

Risk factors associated with Salmonella prevalence, its antibiotic resistance, and egg antibiotic residues in the layer farming environment

et al. 2022

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Background and Aim: Human salmonellosis with non-typhoidal Salmonella remains a global public health concern related to the consumption of contaminated eggs and egg-based products. This study aimed to examine the prevalence of Salmonella, antimicrobial-resistant Salmonella, and egg antibiotic residues concerning risk factors associated with Salmonella contamination in eggs, the layer farming environment, and laying hens kept in battery-cage closed-housing systems. Materials and Methods: This study used a repeated cross-sectional design to collect 488 samples from eggs, laying hens, and the farm environment on one laying farm for Salmonella detection according to ISO 6579:2002/AMD 1:2007. Salmonella-positive samples were further tested for serotype and antimicrobial susceptibility using the disk diffusion test. The layer farm contact person was interviewed at the sampling time to evaluate the risk factors associated with Salmonella contamination using logistic regression analysis. For each month, 24 eggs (144 eggs in total) were also randomly sampled from the collection egg area at the farm for antibiotic residue detection using the European Four Plate Test. Results: The highest Salmonella prevalence rates were in the samples from the layer pen floors, followed by the egg sizing machine (ESM) and eggshells at 65.5%, 52.5%, and 15%, respectively. Salmonella enterica serovar Corvallis was the dominant serovar (48.38%), followed by Mbandaka (37.76%), Braenderup (14.29%), and Typhimurium (4.08%). Rodent presence at the farm and the frequency of changing the disinfectant foot dip were significant factors related to Salmonella contamination on the pen floors (odds ratio [OR]=22.5, 95% confidence interval [CI]=2.11-240.48, p=0.01; OR=24, 95% CI=2.78-206.96, p=0.004, respectively). Hand-washing before sorting and cleaning the ESM were the significant factors (OR=13, 95% CI=1.2-140.73, p=0.04). The most resistant Salmonella isolates were resistant to oxytetracycline. One isolate of S. enterica Typhimurium was resistant to cefotaxime, enrofloxacin, and oxytetracycline. The antibiotic residues in the egg yolks were streptomycin, enrofloxacin, and tetracycline at prevalence rates of 36.11%, 11.81%, and 7.64%, respectively. Streptomycin was the most abundant residue in the albumen and yolk, followed by tetracycline. Conclusion: Salmonella prevalence in layer farming with a closed-housing system is related to effective biosecurity and hygiene issues, such as rodent control, clean farm equipment, and good worker hygiene. In addition, eggs' antibiotic residues may be related to treating antimicrobial-resistant Salmonella isolates and medicated feed with inappropriate antibiotic withdrawal time.

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“…Research by Dec et al (2015) revealed that high intrinsic resistance to ampicillin, amoxicillin, inhibitors of protein synthesis, fluoroquinolone inhibitors of replication, neomycin, tetracycline, lincomycin, doxycycline, tylosin, flumequine and enrofloxacin were observed in Lactobacillus strain. A similar study was also done by Sornplang et al (2016) to investigate the susceptibility of 93 Lactobacillus strains to seven antimicrobial agents and found that more than 50 % of Lactobacillus species were intrinsically resistant to vancomycin, streptomycin, ciprofloxacin, and sulfamethoxazole-trimethoprim. A study was done by Dubourg et al (2015) to characterize the susceptibility levels of Lactobacillus spp.…”

Section: Antibiotic Resistance In Labmentioning

confidence: 83%

Human milk as a potential source for isolation of probiotic lactic acid bacteria: a mini review

Mustakim¹,

Sinawat²,

Salleh³

et al. 2019

Food Res.

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Probiotics are living microorganism that can be employed as a new approach to promote human health. These organisms are found to have attractive means for health due to their probiotic properties particularly in generating antimicrobial activity. Lactobacillus spp. is one of the main genera commonly used for probiotic purpose. Human milk is a potential source of Lactobacillus spp. and one of the criteria that found beneficial is that it is of human origin, which could be more reliable sources to be used in human. Lack of studies on isolation of probiotic bacteria from human milk was reported and some probiotic properties show a variation between strains from different regions and population. Therefore, it is important to carry out the isolation of Lactobacillus spp. from a large number of species in the genera to facilitate the finding of the most competent strain to be incorporated as probiotic agent. Moreover, to ensure suitability and compatibility for human use, the probiotic agent originated from human milk should not be an exception. In addition, certain probiotics show a great correlation with prebiotic existed in human milk to boost their function and may suggest an added value to be a suitable candidate as probiotic. This review provides an overview of studies related to human milk as the promising sources for isolation of probiotic microorganisms.

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Antimicrobial susceptibility of lactic acid bacteria isolated from human and food-producing animal feces in Khon Kaen Province, Thailand

Cited by 11 publications

References 17 publications

Safety and Growth Optimization of Lactic Acid Bacteria Isolated From Feedlot Cattle for Probiotic Formula Design

Safety and Growth Optimization of Lactic Acid Bacteria Isolated From Feedlot Cattle for Probiotic Formula Design

Risk factors associated with Salmonella prevalence, its antibiotic resistance, and egg antibiotic residues in the layer farming environment

Human milk as a potential source for isolation of probiotic lactic acid bacteria: a mini review

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