Antimicrobial Susceptibility of &lt;i&gt;Escherichia coli&lt;/i&gt; Isolated from Feces of Wild Cranes Migrating to Kagoshima, Japan

“…Recent studies have shown that antimicrobial-resistant bacteria are present in different wild animals that have not been exposed to antibiotics [6,7]. Moreover, the resistance genes of bacteria from wild birds may result in increased numbers of resistant bacteria in livestock and humans [8].…”

Section: Introductionmentioning

confidence: 99%

Detection of tetracycline resistance determinant tetA gene and antimicrobial resistance pattern in Escherichia coli isolates recovered from healthy layer chickens

Balasubramaniam¹,

Eswaran²,

Suresh³

et al. 2014

Vet World

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Aim:The aim was to study the occurrence of tetracycline resistance determinant tetA gene, and antibacterial resistance pattern in commensal Escherichia coli recovered from healthy non-clinical layer chickens. Materials and Methods:Twenty-four cloacal swabs were obtained from 15 flocks in five different layer farms located at around Namakkal, which is a place of high-intensity layer chicken rearing in south India. Identification of E. coli was carried out by performing cultural and biochemical tests. Antimicrobial resistance test was carried out using disc diffusion method. The polymerase chain reaction employing tetAC forward and tetAC reverse primers were carried out to detect tetA gene conferring resistance to tetracycline.Result: All the collected cloacal swabs yielded E. coli. Twenty-one isolates (88%) were resistant to tetracycline antibiotic in disc diffusion method. All the isolates showed resistance to more than six antibiotics, which implied existence of multidrug resistant microbiota in intestine of poultry. Only seven (29%) isolates showed the presence of tetA gene indicating the involvement of either other one or more efflux gene(s), namely tetB, tetC and tetD or ribosomal protection encoded by tetM, tetO, tetQ and tetS genes than tetA gene. Conclusion:Based on the presence of tetA gene among tetracycline-resistant bacteria in healthy non-clinical food-producing animals such as layer chickens, it can be significant in human medicine as tetA gene could easily be spread to other bacteria. This kind of phenomenon can be extrapolated in transfer of resistance for other antibacterial essential for treating bacterial infections in human. We conclude that preventing the spread of antimicrobial resistance through direct or indirect contact, consumed food/feed and through the environment is empirical in reduction of failures while treating bacterial infections.

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“…OTC-resistant strains have been detected in hooded cranes (Grus monacha) that inhabit cattle farms in the Kagoshima Prefecture, Japan. The greatest OTC MIC value in hooded cranes was 128 μg/ml (Kitadai, Obi, Yamashita, Murase, & Takase, 2012); in Okinawa rails, we detected a higher resistance to OTC (MIC ≥ 256 μg/ml). Oonaka, Furuhata, Kiuchi, Hara, and Fukuyama (2004) Although the use of ABPC in human therapy is quite rare (Terada, Miyake, & Urase, 2012), 16 t/year of bulk powder has been sold in Japan for use with domestic and pet animals (National Veterinary Assay Laboratory Ministry of Agriculture, Forestry, & Fisheries of Japan, 2012).…”

Section: Re Sults and Discussionmentioning

confidence: 56%

“…OTC‐resistant strains have been detected in hooded cranes ( Grus monacha ) that inhabit cattle farms in the Kagoshima Prefecture, Japan. The greatest OTC MIC value in hooded cranes was 128 μg/ml (Kitadai, Obi, Yamashita, Murase, & Takase, ); in Okinawa rails, we detected a higher resistance to OTC (MIC ≥ 256 μg/ml). Oonaka, Furuhata, Kiuchi, Hara, and Fukuyama () compared AMR bacterium MIC values in samples from the environment and of human clinical origin, and found higher MIC values in human clinical isolates.…”

Section: Resultsmentioning

confidence: 68%

Prevalence of antimicrobial‐resistant Escherichia coli in endangered Okinawa rail (Gallirallus okinawae) inhabiting areas around a livestock farm

Ishibashi

Sumiyama

Kanazawa

et al. 2019

Veterinary Medicine & Sci

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Antimicrobial resistance (AMR) is an important issue for public, animal and environmental health. It has been suggested that livestock farms could be a source origin of AMR, and some wild animals that inhabit this area may play an important role in the spread of AMR in the natural environment. The prevalence of AMR in Escherichia coli was examined from Okinawa rails (Gallirallus okinawae), an endemic bird in Okinawa Main Island, Japan. Forty‐eight faecal samples of wild Okinawa rails were collected from around a livestock farm area (LA), near human settlements, in which a population of the Okinawa rail had newly inhabited for feeding, and a forest area (FA), their natural habitat. Among 16 E. coli‐positive faecal samples collected around LA, 11/16 (69%) showed antimicrobial resistance and five multiple drug resistance patterns were identified. However, among 15 E. coli‐positive faecal samples from FA, 3/15 (20%) showed antimicrobial resistance, and three multiple drug resistance patterns were identified. These results indicate that the endangered Okinawa rail may also play an important role as a potential vector for the spread of AMR in the natural environment. To maintain ecological health, it is imperative that in situ/ex situ conservation projects that include translocation plans for endangered species are aware of these data.

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Antimicrobial Susceptibility of <i>Escherichia coli</i> Isolated from Feces of Wild Cranes Migrating to Kagoshima, Japan

Cited by 16 publications

References 19 publications

Dissemination of antibiotic resistant Enterococcus spp. and Escherichia coli from wild birds of Azores Archipelago

Dissemination of antibiotic resistant Enterococcus spp. and Escherichia coli from wild birds of Azores Archipelago

Detection of tetracycline resistance determinant tetA gene and antimicrobial resistance pattern in Escherichia coli isolates recovered from healthy layer chickens

Prevalence of antimicrobial‐resistant Escherichia coli in endangered Okinawa rail (Gallirallus okinawae) inhabiting areas around a livestock farm

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