Antimicrobial Susceptibility Profile of Coliforms from Bovine Mastitis Cases among Pastoral Herds in Parts of Kaduna State, Nigeria: Curbing the Environmental Health Risk

Makolo, D.; Suleiman, Abubakar; Olonitola, O. S.; Bellò, Marilena; Alfa, M. I.; Ahmadu, I.; Awulu, F. O.

doi:10.9734/ajarr/2019/v3i230084

Cited by 3 publications

(10 citation statements)

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“…This may explain the reports of high resistance of coliform bacteria against them. This study found that 61.0% isolates were resistant to erythromycin; Mbuk et al [ 49 ] and Makolo et al [ 43 ] had more than 75.0% resistance. The use of chloramphenicol in food-producing animals in many countries has been prohibited to avoid the danger of resistance in human medicine [ 62 ].…”

Section: Discussionmentioning

confidence: 73%

“…The prevalence of coliform-associated mastitis among cows in this study was 21.9% and this was higher than 8.8% in Nigeria [ 43 ] and 7.2% in Rwanda [ 44 ]. The high prevalence may indicate contamination from soil and faecal matter.…”

Section: Discussionmentioning

confidence: 73%

“…coli as the predominant among coliforms associated with mastitis,. Makolo et al [ 43 ] and Mbuk et al [ 49 ] in Nigeria reported Klebsiella spp. as the predominant coliform.…”

Section: Discussionmentioning

confidence: 99%

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Prevalence and risk factors of coliform-associated mastitis and antibiotic resistance of coliforms from lactating dairy cows in North West Cameroon

et al. 2022

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Background Coliform bacteria are major causative agents of bovine mastitis, a disease that has devastating effect on dairy animal health and milk production. This cross-sectional study, carried out in the North West region of Cameroon, sought to determine the prevalence of bovine mastitis, coliforms associated with bovine mastitis, risk factors for infection and the antibiotic resistance pattern of coliform bacterial isolates. Materials and methods A total of 1608 udder quarters were sampled from 411 cows using a questionnaire, clinical examination, California Mastitis Test and milk culture. Primary isolation of coliform bacteria was done on MacConkey agar while identification of coliforms employed Gram-staining and biochemical testing. Each coliform bacterial isolate was challenged with 11 antibiotics using the Kirby-Bauer disc diffusion method. Results The prevalence of mastitis was 53.0% (218/411) and 33.1% (532/1608) at the cow- and quarter-levels respectively. Overall, 21.9% (90/411) cows and 8.2% (132/1608) udder quarters showed coliform mastitis. Escherichia coli was isolated in 7.0% of mastitis milk, and other coliforms isolated were Enterobacter cloacae (12.6%), Klebsiella pneumoniae (2.4%), Enterobacter sakazakii (1.1%), Klebsiella oxytoca (0.8%), Citrobacter freudii (0.4%), Serratia ficaria (0.4%) and Serratia liquefaciens (0.2%). Lactation stage, breed, history of mastitis and moist/muddy faeces contaminated environment were significantly associated (P-value < 0.05) with coliform mastitis. Coliform isolates (99.0%; 203/205) were resistant to at least one antibiotic tested. Amoxicillin had the highest resistance (88.8%) while norfloxacin had the least resistance (3.4%). Multidrug resistance was exhibited by 52.7% (108/205) of the isolates in a proportion of 27.8% Enterobacter cloacae, 10.7% E. coli, 6.3% Klebsiella pneumoniae, 2.9% Enterobacter sakazakii, 2.0% Klebsiella oxytoca, 1.0% Citrobacter freundii, 1.0% Serratia ficaria, 0.5% Serratia liquefaciens and 0.5% Serratia odorifera. Conclusion Results indicate a need to educate these dairy farmers about mastitis (particularly subclinical), proper hygiene methods in milking and the public health implications of consuming contaminated raw milk.

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Section: Discussionmentioning

confidence: 73%

Section: Discussionmentioning

confidence: 73%

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Prevalence and risk factors of coliform-associated mastitis and antibiotic resistance of coliforms from lactating dairy cows in North West Cameroon

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“…Makolo et. al (41). stated that they isolated six E. coli and seven Klebsiella pneumonia from 147 milk samples.…”

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confidence: 99%

“…According to these results, it was considered that multiple drug resistance may spread among the isolates. In K. oxytoca isolates, the highest Makolo et al(41) stated high multiple resistance to tetracycline, penicillin, and erythromycin. Bhat et al(8) determined that the isolates were susceptible mostly to enrofloxacin and gentamicin but all the isolates were resistant to penicillin.…”

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confidence: 99%

First detection of carbapenem resistance in Enterobacteriaceae isolates isolated from dairy cows’ mastitis infection in Türkiye

Babacan

2022

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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With this study, carbapenem resistance genes were declared for the first time in Enterobacteriaceae isolates isolated from dairy cows' mastitis infection in Turkey. In the bacteriological examination of 212 milk samples, 14 (6.60%) E. coli, three (1.41%) Klebsiella oxytoca, and two (0.94%) Klebsiella pneumonia were isolated.At least two E. coli isolates were found to be resistant to all of the antibiotics used in the antibiogram test. The highest resistance was found against cefotaxime and amoxicillin in K. oxytoca isolates. According to the results of PCR targeting blaCTX-M, blaTEM, and blaSHV genes, the blaCTX-M gene was detected in one K. oxytoca and four E. coli isolates, which were found ESBL positive. According to the results of PCR targeting carbapenem and colistin resistance genes, the IMP gene was detected in four E.coli, one K. oxytoca, and one K. pneumonia isolates. OXA-48-like gene was detected in two E. coli isolates. This two E. coli isolates were also IMP gene positive. While NDM gene was detected in two E. coli, KPC gene was detected in one E. coli isolate. One of the colistin resistance genes, mcr-1 was detected in two E.coli strains with PCR. This study showed that ESBL production and carbapenem resistance in Enterobacteriaceae family strains to become prevalent and increasing, especially among E. coli isolates.Furthermore, identification of multiple antibiotic resistance in the isolates indicated that antibiotic resistance also spread rapidly and increased.

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IN SILICO AND IN VITRO ANTIMICROBIAL TESTING OF AQUEOUS EXTRACT OF Vernonia amygdalina AGAINST Escherichia coli ISOLATED FROM WISTAR RATS INFECTED WITH Trypanosoma congolense

Tom-Otu

Omowaye

Makolo

et al. 2022

OJBR

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The study was carried out to investigate the in silico and in vitro antimicrobial activity of Vernonia amygdalina aqueous extract (VAAE) against Escherichia coli on rats already infected with Trypanosoma congolense. Forty Wistar rats of 16 weeks old were used, and were randomly selected into six groups, where n = 5 in groups A, B, D, E and F; n = 15 in group C. Group A: uninfected-untreated; group B: uninfected-treated (500 mg/kgbwt VAAE); group C: infected-untreated; groups D: infected-treated (100 mg/kgbwt VAAE), E: infected-treated (300 mg/kgbwt (VAAE) and F: infected-treated (500 mg/kgbwt (VAAE). Using docking simulation, a number of phytochemicals were screened and identified in the VAAE used in the study. The findings have given an indication of in vitro antimicrobial activity of aqueous extract of Vernonia amygdalina leaves against Escherichia coli. Docking with topoisomerase iv (3FV5) revealed that 3 compounds (−8.5 to – 8.3 kcal/mol) with docking scores lower than the co-crystalized PBE (-6.1kcal/mol). The five topmost docked compounds including vernonioside A22, vernonioside A42, luteolin-7-rutinoside, vernonioside D2 and proline betaine (PBE) with the lowest docking scores exhibited the highest binding tendency with topoisomerase iv, upon interaction analysis. Residues involved in the intermolecular interaction include Asp69, Ile116, Ser117, Leu94, Gly73 Leu89, Arg93, Ile90, Glu46 and Val39 (h-bonds), Val118, Ile90, Met74, Gly73, Arg72, Pro75, Val39, Val165 and Tyr505 (π-π stacking interactions) and Glu46. Our findings revealed the stability of vernonioside A22, vernonioside A42, luteolin-7-rutinoside, vernonioside D2 and proline betaine (PBE) on the target topoisomerase iv in the target organism, which in support of the in vitro study has also shown that Vernonia amygdalina leaves have antimicrobial potential and as such be considered as a formidable source for the search for new drugs against bacteria.

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Antimicrobial Susceptibility Profile of Coliforms from Bovine Mastitis Cases among Pastoral Herds in Parts of Kaduna State, Nigeria: Curbing the Environmental Health Risk

Cited by 3 publications

References 16 publications

Prevalence and risk factors of coliform-associated mastitis and antibiotic resistance of coliforms from lactating dairy cows in North West Cameroon

Prevalence and risk factors of coliform-associated mastitis and antibiotic resistance of coliforms from lactating dairy cows in North West Cameroon

First detection of carbapenem resistance in Enterobacteriaceae isolates isolated from dairy cows’ mastitis infection in Türkiye

IN SILICO AND IN VITRO ANTIMICROBIAL TESTING OF AQUEOUS EXTRACT OF Vernonia amygdalina AGAINST Escherichia coli ISOLATED FROM WISTAR RATS INFECTED WITH Trypanosoma congolense

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