Antimicrobial Treatment Provides a Competitive Advantage to Mycobacterium abscessus in a Dual-Species Biofilm with Pseudomonas aeruginosa

Rodríguez-Sevilla, Graciela; Crabbé, Aurélie; García-Coca, Marta; Aguilera-Correa, John Jairo; Esteban, Jaime; Pérez‐Jorge, Concepción

doi:10.1128/aac.01547-19

Cited by 14 publications

(18 citation statements)

References 38 publications

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“…Regarding microbial intra-kingdom interactions, the antimicrobial susceptibility of different bacterial species growing inside polymicrobial biofilms has been investigated (Pompilio et al, 2015 ; Cendra et al, 2019 ; Rodríguez-Sevilla et al, 2019 ). Cross-kingdom interactions impact on antimicrobial susceptibility is still poorly studied, but some authors have described the antimicrobial susceptibility of Candida albicans yeast in polymicrobial biofilm with Staphylococcus aureus (Harriott and Noverr, 2009 ; Kong et al, 2016 ; Rogiers et al, 2018 ) or Cutibacterium acnes (Bernard et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Modulated Response of Aspergillus fumigatus and Stenotrophomonas maltophilia to Antimicrobial Agents in Polymicrobial Biofilm

Roisin

Melloul

Woerther

et al. 2020

Front. Cell. Infect. Microbiol.

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Introduction: The complexity of biofilms constitutes a therapeutic challenge and the antimicrobial susceptibility of fungal-bacterial biofilms remains poorly studied. The filamentous fungus Aspergillus fumigatus (Af) and the Gram-negative bacillus Stenotrophomonas maltophilia (Sm) can form biofilms and can be co-isolated from the airways of cystic fibrosis (CF) patients. We previously developed an in vitro biofilm model which highlighted the antibiosis effect of Sm on Af, which was dependent on the bacterial fitness. The aim of the present study was to investigate the in vitro susceptibility of Af and Sm in mono- or polymicrobial biofilms to five antimicrobial agents alone and in two-drug combinations. Methods: Af and Sm clinical reference strains and two strains from CF sputa were tested through a planktonic and biofilm approaches. Af, Sm, or Af-Sm susceptibilities to amphotericin B (AMB), itraconazole (ITC), voriconazole (VRC), levofloxacin (LVX), and rifampicin (RFN) were evaluated by conventional planktonic techniques, crystal violet, XTT, qPCR, and viable plate count. Results: Af planktonic cells and biofilms in formation were more susceptible to AMB, ITC, and VRC than Af mature biofilms. Af mature biofilms were susceptible to AMB, but not to ITC and VRC. Based on viable plate count, a lower concentration of LVX and RFN was required to reduce Sm cell numbers on biofilms in formation compared with mature biofilms. The antibiosis effect of Sm on Af growth was more pronounced for the association of CF strains that exhibited a higher fitness than the reference strains. In Af-Sm biofilms, the fungal susceptibility to AMB was increased compared with Af biofilms. In contrast, the bacterial susceptibility to LVX decreased in Af-Sm biofilms and was fungal biomass-dependent. The combination of AMB (64 μg/mL) with LVX or RFN (4 μg/mL) was efficient to impair Af and Sm growth in the polymicrobial biofilm. Conclusion: Sm increased the Af susceptibility to AMB, whereas Af protected Sm from LVX. Interactions between Af and Sm within biofilms modulate susceptibility to antimicrobial agents, opening the way to new antimicrobial strategies in CF patients.

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Section: Introductionmentioning

confidence: 99%

Modulated Response of Aspergillus fumigatus and Stenotrophomonas maltophilia to Antimicrobial Agents in Polymicrobial Biofilm

Roisin

Melloul

Woerther

et al. 2020

Front. Cell. Infect. Microbiol.

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“…Moreover, the possibility of dual biofilms (and probably polymicrobial ones) that include M. abscessus [ 55 , 110 , 111 ] represents a new challenge for the management of these patients. The relationships between the different species are not well understood yet, but the implications of this fact in the clinical management of patients could be important, and the diagnosis and antibiotic selection should consider this possibility.…”

Section: Discussionmentioning

confidence: 99%

“…In vitro conditions that mimic the airways of CF patients appear to facilitate the establishment of M. abscessus infection, and the removal of magnesium from the environment may affect the ability of the pathogen to establish infection [ 54 ]. Moreover, some studies showed that P. aeruginosa inhibited M. abscessus biofilm formation under control conditions and that antimicrobial treatment selectively targeting P. aeruginosa decreased this competitive interaction, thereby increasing M. abscessus survival [ 55 ].…”

Section: Models Of Biofilmsmentioning

confidence: 99%

Updated Review on the Mechanisms of Pathogenicity in Mycobacterium abscessus, a Rapidly Growing Emerging Pathogen

2022

Self Cite

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In recent years, Mycobacterium abscessus has appeared as an emerging pathogen, with an increasing number of disease cases reported worldwide that mainly occur among patients with chronic lung diseases or impaired immune systems. The treatment of this pathogen represents a challenge due to the multi-drug-resistant nature of this species and its ability to evade most therapeutic approaches. However, although predisposing host factors for disease are well known, intrinsic pathogenicity mechanisms of this mycobacterium are still not elucidated. Like other mycobacteria, intracellular invasiveness and survival inside different cell lines are pathogenic factors related to the ability of M. abscessus to establish infection. Some of the molecular factors involved in this process are well-known and are present in the mycobacterial cell wall, such as trehalose-dimycolate and glycopeptidolipids. The ability to form biofilms is another pathogenic factor that is essential for the development of chronic disease and for promoting mycobacterial survival against the host immune system or different antibacterial treatments. This capability also seems to be related to glycopeptidolipids and other lipid molecules, and some studies have shown an intrinsic relationship between both pathogenic mechanisms. Antimicrobial resistance is also considered a mechanism of pathogenicity because it allows the mycobacterium to resist antimicrobial therapies and represents an advantage in polymicrobial biofilms. The recent description of hyperpathogenic strains with the potential interhuman transmission makes it necessary to increase our knowledge of pathogenic mechanisms of this species to design better therapeutic approaches to the management of these infections.

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“…The physiological factors that contribute to AMR in biofilms are complex. A study on antibiotic treatment of Pseudomonas aeruginosa , a major cystic fibrosis pathogen, demonstrated that it created optimal conditions for the establishment of Mycobacterium abscessus infection [ 29 ]. The P. aeruginosa inhibited M. abscessus biofilm formation until antimicrobial therapy selectively targeting P. aeruginosa diminished this competitive interaction.…”

Section: Antimicrobial Resistancementioning

confidence: 99%

Infectious Diseases Impact on Biomedical Devices and Materials

Brigmon

2022

Biomedical Materials & Devices

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Infectious diseases and nosocomial infections may play a significant role in healthcare issues associated with biomedical materials and devices. Many current polymer materials employed are inadequate for resisting microbial growth. The increase in microbial antibiotic resistance is also a factor in problematic biomedical implants. In this work, the difficulty in diagnosing biomedical device-related infections is reviewed and how this leads to an increase in microbial antibiotic resistance. A conceptualization of device-related infection pathogenesis and current and future treatments is made. Within this conceptualization, we focus specifically on biofilm formation and the role of host immune and antimicrobial therapies. Using this framework, we describe how current and developing preventative strategies target infectious disease. In light of the significant increase in antimicrobial resistance, we also emphasize the need for parallel development of improved treatment strategies. We also review potential production methods for manufacturing specific nanostructured materials with antimicrobial functionality for implantable devices. Specific examples of both preventative and novel treatments and how they align with the improved care with biomedical devices are described.

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Antimicrobial Treatment Provides a Competitive Advantage to Mycobacterium abscessus in a Dual-Species Biofilm with Pseudomonas aeruginosa

Cited by 14 publications

References 38 publications

Modulated Response of Aspergillus fumigatus and Stenotrophomonas maltophilia to Antimicrobial Agents in Polymicrobial Biofilm

Modulated Response of Aspergillus fumigatus and Stenotrophomonas maltophilia to Antimicrobial Agents in Polymicrobial Biofilm

Updated Review on the Mechanisms of Pathogenicity in Mycobacterium abscessus, a Rapidly Growing Emerging Pathogen

Infectious Diseases Impact on Biomedical Devices and Materials

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