Antimutagenic activity of ipriflavone against the DNA-damage induced by cyclophosphamide in mice

Abstract

In the present study we evaluated the potential of ipriflavone against the cytotoxic and mutagenic effects induced by cyclophosphamide chemotherapeutic agent in bone marrow cells of mice, using the micronucleus assay in vivo on cells of bone marrow. The study was performed following three protocols: pre-treatment, simultaneous treatment and post treatment. The results demonstrated that ipriflavone has a protective effect against mutagenicity induced by cyclophosphamide in the pre-treatment and post-treatment and against the cytotoxicity in all treatments. There was variation between the genders in some of the experimental groups. To evaluate their possible mechanisms of action, it was performed the DPPH assay, which showed no ability to donate hydrogens, suggesting that it acts through other mechanisms. Due to its ability to prevent chromosomal damage, ipriflavone is likely to open an interest field concerning its possible the use in clinical applications.

In Vitro Biotransformation, Safety, and Chemopreventive Action of Novel 8-Methoxy-Purine-2,6-Dione Derivatives

“…Interestingly, all the investigated compounds showed antimutagenic potential, which means that they have chemopreventive activity. Therefore, they might potentially delay, inhibit, or even reverse carcinogenesis [ 38 ]. Nevertheless, the mechanism that explains their chemopreventive action is not the free radical scavenging activity, as they showed a low or non-existent capacity to quench DPPH radical in DPPH assay performed.…”

“…The capacity of selected 8-methoxy-purine-2,6-dione derivatives to scavenge stable free radical DPPH was assessed [ 22 , 38 – 42 ]. A blank and a triplicate of 10 different concentrations of each compound or of each positive control (gallic and ascorbic acids) were evaluated in the experiment.…”

Antigenotoxic effects of a polyherbal drug septilin against the genotoxicity of cyclophosphamide in mice

“…CP is a well known cytotoxic genotoxin. One of the useful strategies for the protection against chromosomal damage induced by this type of agents is using the compounds which are capable of preventing the future damages or repairing an already made damage [18] . In the present investigation, septilin was used to study its effect on the cytogenetic damage induced by CP.…”

“…Since CP is a well-known mutagen/genotoxin, in the present study it was used as a positive mutagen. There are several reports on the use of CP to evaluate the anticlastogenic/antigenotoxic effects of various natural compounds and other chemicals [87] , [61] , [28] , [92] , [32] , [33] , [18] , [70] . [42] reported the anticlastogenic effect of Ricinus communis extract against CP induced clastogenicity in mice bone marrow cells.…”

Dietary supplementation with ipriflavone decreases hepatic iron stores in wild type mice

“…While ipriflavone has estrogenic effects, it is less stimulatory to the endometrium than estradiol [13]. Ipriflavone, taken as an oral supplement, has been shown to increase bone mineral density in postmenopausal women [14] and to have antioxidant properties [15]. …”