Antimutagenic Activity of Isoflavone from Pueraria lobata

Abstract

A methanol extract from Pueraria lobata showed a suppressive effect on umu gene expression of the SOS response in Salmonella typhimurium TA1535/pSK1002 against the mutagen 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide (furylfuramide). The methanol extract from P. lobata was re-extracted with hexane, dichloromethane, ethyl acetate, butanol, and water, respectively. A suppressive compound in the dichloromethane and ethyl acetate extract fractions was isolated by SiO(2) column chromatography and identified as tectorigenin (1) by EI-MS and (1)H and (13)C NMR spectroscopy. Compound 1 and its methylated derivative [7,4'-di-O-methyltectorigenin (2)] had the suppressive effects on umu gene expression of the SOS response in Salmonella typhimurium TA1535/pSK1002 against furylfuramide, 4-nitroquinoline-1-oxide, N-methyl-N'-nitrosoguanidine, and activated Trp-P-1, which do not require live metabolic activation by S9. These compounds also showed suppression of SOS-inducing activity against Trp-P-1 and AfB(1), which requires liver metabolizing enzymes. In addition to the antimutagenic activities of these compounds against furylfuramide, Trp-P-1 and activated Trp-P-1 were also assayed by an Ames test using S. typhimurium TA100.

Antigenotoxic activity of naturally occurring furanocoumarins

“…Inhibition of the metabolic enzymes such as CYP1A1 and CYP1A2, is implicated in the antigenotoxic mechanism of oltiplaz [Langouet et al, 2000], isothiocyanate [Goosen et al, 2001], resveratrol [Chun et al, 1999], and chlorophylines [Yun et al, 1995]. Antimutagenic effects of some naturally occurring compounds against mutagens by using umu test have been investigated [Miyazawa et al, 2000;Miyazawa et al, 2001].…”

Antitumor activity of spinasterol isolated from Pueraria roots

“…For example, the Thai vine, Pueraria mirifica, is used as a rejuAntitum or activity of spinasterol isolated from Pueraria roots venator and aphrodisiac (Bradbury and White, 1954;Cain, 1960), and other Pueraria species are reported to have pharmacological activity (Harada and Ueno, 1975;Qicheng, 1980;Lai and Tang, 1989;Keung and Vallee, 1998;Miyazawa et al, 2001). Pueraria root is a potent source of phytoestrogen with estrogen-like biological activity.…”

Cytoprotective Effects of KIOM-79 on Streptozotocin Induced Cell Damage by Inhibiting ERK and AP-1

“…12) Pueraria lobata reportedly has antimutagenic, antidiabetic and antioxidant effects. [13][14][15] Glycyrrhiza uralensis has detoxification, antiulcer, antiinflammation, antiviral, antiatherogenic, and anticarcinogenic effects, 16) and Euphorbia pekinensis has for antiviral and cytotoxic activities. 17,18) Recently, KIOM-79 has suggested possible anti-inflammatory properties by blocking NFkB/Rel and p38 kinase activation.…”