Antimutagenic and mutagenic activities of some terpenes in the bacterial reverse mutation assay

Clastogenicity of Beta-Caryophyllene in Mouse

“…1) In regard to genotoxic studies in mammalian cells, no previous studies on the BC potential have been made. However, contrary to our negative results and those of Di Soto et al in bacteria, 2) other sesquiterpenes have given positive results: this is the case of the compound lactone lynchnopholide that was reported as a chromosome damaging agent in in vitro and in vivo assays, and was shown to be cytotoxic in vitro 11) ; another such case is that of parthenin which presented a strong capacity to induce micronuclei in vivo. 12) These divergent results suggest the relevance of the chemical structure in the biological effect of sesquiterpenes, and indicate as well the importance of using various test models to reach a valid conclusion.…”

“…Studies on the group have given varying results: for example, in a bacterial assay linalool and BC showed no mutagenicity but lynalil acetate did; on the other hand, linalool was not antimutagenic whereas BC gave positive results; and finally, the toxicity of linalool and lynalil acetate proved to be stronger than BC. 2) Cytotoxic data about sesquiterpenes in mammalian cells have also varied; for example, in cultivated cells a stronger effect of alpha humulene is generally shown, in comparison with that of alpha or gamma caryophyllene, BC being the less toxic of these agents. 1) In regard to genotoxic studies in mammalian cells, no previous studies on the BC potential have been made.…”

“…The authors evaluated its mutagenic potential by using the bacterial reverse mutation assay and obtained negative results 2) ; therefore, the study of its genotoxic potential in an in vivo assay was necessary. In this investigation we determined the effect of BC on the induction of micronucleated polychromatic erythrocytes (MNPE) in mouse, as well as its cytotoxicity by detecting its capacity to modify the bone marrow polychromatic erythrocyte rate in the same model.…”

Efficient, large-scale synthesis and preclinical studies of MRS5698, a highly selective A3 adenosine receptor agonist that protects against chronic neuropathic pain

“…For all the protocols, the plates were incubated at 37°C for 72 h and scored for histidine-or trp-independent revertant colonies and viable cells. The percentage of inhibition of the mutagenic effect was calculated according to Di Sotto et al [18]. Antimutagenicity was considered moderate with an inhibitory effect in the range of 25−40 % and strong when the inhibition was higher than 40 %; when the inhibitory effect was lower than 25 %, it was considered weak and not recognized as a positive result [18].…”

Mutagenicity, genotoxicity, and scavenging activities of extracts from the soft coral Chromonephthea braziliensis: a possibility of new bioactive compounds

“…In the same way, α-pinene, a monoterpene, can cause oxidative damage (Singh et al, 2006). Assays for the antimutagenic and mutagenic activity of linalool, linalyl acetate, and β-caryophyllene -all terpenes -showed positive responses to mutagenicity for linalyl acetate and antimutagenic activity for linalool and β-caryophyllene (Di Sotto et al, 2008).…”