Antimutagenicity and induction of antioxidant defense by flavonoid rich extract of Myrcia bella Cambess. in normal and tumor gastric cells

Serpeloni, Juliana Mara; Specian, Ana Flávia Leal; Ribeiro, Diego Luís; Tuttis, Katiuska; Vilegas, Wagner; Martínez‐López, Wilner; Dokkedal, Anne Lígia; Saldanha, Luiz Leonardo; Cólus, Ilce Mara de Syllos; Varanda, Eliana Aparecida

doi:10.1016/j.jep.2015.11.003

Cited by 35 publications

(28 citation statements)

References 45 publications

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“…To evaluate the cell proliferation, the best choice is to measure total cellular protein content, which is not dependent of cell metabolism (Skehan et al 1990;Van Tonder et al 2015). For this, sulforhodamine B (SRB) or bromophenol blue (Skehan et al 1990;Cilião et al 2015;Serpeloni et al 2015) can be used as dye of cellular total protein content. The results obtained using the cell proliferation curves with bromophenol blue suggest that the three concentrations of all extracts evaluated did not alter the proliferative rate of either cell line in long-term treatment (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…The technique was performed as described by Serpeloni et al (2015) and Ribeiro et al (2016). The results are expressed as the percentage of viable cells concerning the negative control group (PBS), which was considered to represent 100 % viability.…”

Section: Mtt Assaymentioning

confidence: 99%

“…A proliferation curve based on the determination of the total protein content was also performed using the technique proposed by Da Costa Lopes et al 2000, where the proteins were dyed with 1 % bromophenol blue. The steps were the same as performed by studies of Cilião et al (2015) and Serpeloni et al (2015).…”

Section: Cell Proliferation Curvesmentioning

confidence: 99%

“…The cell cycle analysis was performed as described by Ribeiro et al (2016) and Serpeloni et al (2015), 3.0 9 10 5 cells were seeded in complete culture medium in a final volume of 2.5 mL in 12-well plates and were treated according to the protocol described before. Control groups were also included (positive DXR 0.2 lg/mL for GAS cells and B[a]P 5.04 lg/mL for HepG2 cells), negative (PBS) and solvent (SV).…”

Section: Flow Cytometrymentioning

confidence: 99%

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LDH, proliferation curves and cell cycle analysis are the most suitable assays to identify and characterize new phytotherapeutic compounds

et al. 2016

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Brazilian flora biodiversity has been widely investigated to identify effective and safe phytotherapeutic compounds. Among the investigated plant species, the Byrsonima genus exhibits promising biological activities. This study aimed at evaluating the cytotoxicity of B. correifolia, B. verbascifolia, B. fagifolia and B. intermedia extracts using different assays in two cell lines (primary gastric and HepG2 cells). The different extract concentrations effects on cell viability were assayed using the MTT, aquabluer, neutral red and LDH assays. Non-cytotoxic concentrations were selected to generate cell proliferation curves and to assess cell cycle kinetics by flow cytometry. Byrsonima extracts differentially affected cell viability depending on the metabolic cellular state and the biological parameter evaluated. B. fagifolia and B. intermedia extracts exhibited lower cytotoxic effects than B. correifolia and B. verbascifolia in all assays. The results obtained with LDH and flow cytometry assays were more reliable, suggesting that they can be useful in the screening for herbal medicine and to further characterize these extracts as phytotherapeutic compounds.

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Section: Discussionmentioning

confidence: 99%

Section: Mtt Assaymentioning

confidence: 99%

Section: Cell Proliferation Curvesmentioning

confidence: 99%

Section: Flow Cytometrymentioning

confidence: 99%

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LDH, proliferation curves and cell cycle analysis are the most suitable assays to identify and characterize new phytotherapeutic compounds

et al. 2016

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“…After all protocols of treatments (no associated and associated), the cells were incubated for 30 h with cytochalasin B (3 lg/mL). The method used in this technique to slide preparation followed the same method described by our research group (Ribeiro et al 2015;Serpeloni et al 2015).…”

Section: Mtt Assaymentioning

confidence: 99%

Phytochemical study and evaluation of cytotoxicity, mutagenicity, cell cycle kinetics and gene expression of Bauhinia holophylla (Bong.) Steud. in HepG2 cells in vitro

et al. 2017

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Bauhinia holophylla (Bong.) Steud. (Fabaceae) is a plant used in Brazilian folk medicine to treat diabetes and inflammation. This study evaluated the phytochemical properties, cytotoxic, apoptotic, mutagenic/antimutagenic effects and alterations in gene expression (RNAm) in HepG2 cells treated with the B. holophylla extract. The phytochemical profile highlight the presence of flavonoids isorhamentin and quercetin derivates. The MTT assay was used to evaluate the cytotoxicity of different concentrations for different treatment times. Three concentrations (7.5, 15, 30 µg/mL) were chosen for assessment of apoptosis (AO/EB), mutagenicity (micronucleus), and cell cycle kinetics (flow cytometry). Thereafter, the concentration of 7.5 µg/mL was chosen to evaluate the protective effects against DNA damage induced by benzo[a]pyrene (B[a]P). At concentrations higher than 7.5 µg/mL (between 10 and 50 µg/mL), the extract was cytotoxic, induced apoptosis, and caused antiproliferative effects. However, it did not induce micronucleus and a reduction of apoptotic and micronucleated cells was observed in treatments that included the extract and B[a]P. The protective effect is attributable to the presence of flavonoids, described as antioxidants, inhibitors of DNA adduct and activators of detoxifying enzymes. The results of the present study such as absence of cytotoxic and mutagenic effects and protective effects against known carcinogens suggest that B. holophylla has potential for use soon as herbal medicine.

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Aglycone flavonoid brachydin A shows selective cytotoxicity and antitumoral activity in human metastatic prostate (DU145) cancer cells

et al. 2021

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Antimutagenicity and induction of antioxidant defense by flavonoid rich extract of Myrcia bella Cambess. in normal and tumor gastric cells

Cited by 35 publications

References 45 publications

LDH, proliferation curves and cell cycle analysis are the most suitable assays to identify and characterize new phytotherapeutic compounds

LDH, proliferation curves and cell cycle analysis are the most suitable assays to identify and characterize new phytotherapeutic compounds

Phytochemical study and evaluation of cytotoxicity, mutagenicity, cell cycle kinetics and gene expression of Bauhinia holophylla (Bong.) Steud. in HepG2 cells in vitro

Aglycone flavonoid brachydin A shows selective cytotoxicity and antitumoral activity in human metastatic prostate (DU145) cancer cells

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