Antimutagenicity in cultured mammalian cells

Kuroda, Yukiaki; Jain, Ajay K.; Tezuka, Hiroyasu; Kada, Tsuneo

doi:10.1016/0027-5107(92)90064-9

Cited by 77 publications

(52 citation statements)

References 23 publications

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“…The protective effect of aqueous extracts of any medicinal products was determined by testing their antimutagenicity potential. Our results are supported by the fact that this product showed no mutagenic activity in the previous studies [2,23].…”

Section: Discussionsupporting

confidence: 90%

“…The aqueous extracts of PM and BF can act directly on compounds that induce mutations in DNA, chemically or enzymatically inactivating them, may inhibit the metabolic activation of promutagenic agents, or may scavenge reactive molecules, as explained [23]. Thus, the considerable presence of antimutagen in Liv52 certainly contributed to their effective antimutagenic activity.…”

Section: Discussionmentioning

confidence: 96%

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Antimutagenic Potential of Liv52 by Ames Salmonella/Mammalian: Microsome Mutagenicity Assay

Manipura

2017

Asian J Pharm Clin Res

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Objective: Liv52, a nontoxic herbal preparation is reported to be clinically active in hepatotoxicity and a wide range of hepatic disorders. This study was undertaken to evaluate the mutagenic/antimutagenic potential of Liv52 using Salmonella mutagenicity test. Methods:Ames Salmonella/Mammalian -microsome mutagenicity test was used to evaluate the mutagenic or antimutagenic potential of Liv52. Salmonella typhimurium tester strains TA1537, TA1538, TA100, and TA102 were used for mutagenicity testing. The antimutagenicity study was carried out in tester strains TA1538 and TA100 against various standard mutagens with and without metabolic activation.Results: Liv52 did not show any mutagenic potential both with and without metabolic activation, whereas in TA1538 and TA 100 tester strains, Liv52 showed 48.4% and 47.2% of inhibition of his + revertants induced by 4-nitro-O-phenylenediamine, respectively. Further with metabolic activation in TA1538, Liv52 showed 99.8%, 99.8%, and 100% inhibition of his + revertants induced by 2-aminofluorene, 2-anthranine, and cigarette smoke condensate, respectively. In TA100 maximum of 100%, 100%, 97.7%, and 100% inhibition of his + revertants induced by 2-aminofluorene, 2-anthranine, benzo(a)pyrene, and cigarette smoke condensate, respectively, were observed. A significant enhancement of inhibition of his + revertants induced by all the above said mutagens were observed in preincubation modification method. Conclusion:Liv52 was found to be nonmutagenic in Salmonella assay, whereas manifested the antimutagenic potential both with and without metabolic activation. The enhanced antimutagenic activity of Liv52 on preincubation indicates that the antimutagenic factor(s) may be desmutagenic in nature. The exact mechanism by which Liv52 exerts antimutagenic potential is not known. The possibility of having diverse antimutagenic factors in Liv52 which act by different mechanisms are strongly indicated.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 96%

Antimutagenic Potential of Liv52 by Ames Salmonella/Mammalian: Microsome Mutagenicity Assay

Manipura

2017

Asian J Pharm Clin Res

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“…According to Kuroda et al (1992) and Kojima et al (1992), the differences found in the three different protocols used in the present study could lead to a better manner of examining the antimutagenicity mechanisms of tested substances. They may act directly on the mutagenic molecule by inactivating it (desmutagens) or, indirectly, by blocking the mutagenic process by increasing the fidelity in DNA replication or by stimulating DNA damage error-free repair (bio-antimutagenesis) (De Flora, 1998).…”

Section: Discussionmentioning

confidence: 94%

Genotoxic and antigenotoxic effects of organic extracts of mushroom Agaricus blazei Murrill on V79 cells

et al. 2005

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Agaricus blazei Murrill, popularly known as the sun mushroom, is a native mushroom in SP, Brazil, that has been widely used in the treatment of cancer and many other pathologies in different parts of the world. A water-soluble protein-polysaccharide complex (1 ® 6)b-D-glucan has been isolated from its fruiting body that showed immune-modulation activity. From organic extracts, linoleic acid has been isolated and determined to be the main substance with antimutagenic activity. Using both the micronucleus (MN) and comet (single cell microgel electrophoresis) assays, this study determined the genotoxic and antigenotoxic potential of A. blazei (AB) obtained from commercial sources or the following strains: a) strains AB 97/29 (young and sporulated phases); b) a mixture taken from AB 96/07, AB 96/09 and AB 97/11 strains; and c) commercial mushrooms from Londrina, PR and Piedade, SP, designated as AB PR and AB SP, respectively. The extracts from these mushrooms were isolated in chloroform:methanol (3:1) and used in vitro at three different concentrations. V79 cells (Chinese hamster lung cells) were exposed to the extracts under pre-, simultaneous and post-treatment conditions, combined with methyl methanesulfonate (MMS). Under the circumstances of this study, these organic extracts did not show any genotoxic or mutagenic effects, but did protect cells against the induction of micronuclei by MMS.

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“…Anticarcinogens will be organized by mode of action, adapted from the schemata found in a recent review of antimutagenic agents in the diet (5). Detailed information on the various test systems used to detect antimutagenicity is provided in other recent works (6)(7)(8) (11). This results in a decrease in the number of mutants recovered after exposure of a cell culture to mutagen.…”

Section: Proposed Mechanisms For Dietary Anticarcinogensmentioning

confidence: 99%

Dietary modifiers of carcinogenesis.

Kohlmeier¹,

Simonsen²,

Mottus³

1995

Environ Health Perspect

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Dietary components express a wide range of activities that can affect carcinogenesis. Naturally occurring substances in foods have been shown in laboratory experiments to serve as dietary antimutagens, either as bioantimutagens or as desmutagens. Dietary desmutagens may function as chemical inactivaters, enzymatic inducers, scavengers, or antioxidants. Dietary components may also act later in the carcinogenic process as tumor growth suppressors. Examples of dietary factors acting in each of these stages of carcinogenesis are presented, and potential anticarcinogens such as the carotenoids, tocopherols, phenolic compounds, glucosinolates, metal-binding proteins, phytoestrogens, and conjugated linoleic acid are discussed. Individual foods typically contain multiple potential anticarcinogens. Many of these substances can influence carcinogenesis through more than one mechanism. Some substances exhibit both anticarcinogenic and carcinogenic activity in vitro, depending on conditions. Epidemiologic research indicates that high fruit and vegetable consumption is associated with lower cancer risk. Little research has focused on the effects of single substances or single foods in man. Realization of the potential of foodborne substances to reduce the human burden of cancer will only be achieved with better measurement of dietary exposures and funding of multidisciplinary research in this area commensurate with its importance. -Environ Health Perspect 103(Suppl 8): 177-184 (1995)

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Antimutagenicity in cultured mammalian cells

Cited by 77 publications

References 23 publications

Antimutagenic Potential of Liv52 by Ames Salmonella/Mammalian: Microsome Mutagenicity Assay

Antimutagenic Potential of Liv52 by Ames Salmonella/Mammalian: Microsome Mutagenicity Assay

Genotoxic and antigenotoxic effects of organic extracts of mushroom Agaricus blazei Murrill on V79 cells

Dietary modifiers of carcinogenesis.

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