Antimycobacterial activity of ionic fullerene derivatives

Bosi, Susanna; Ros, Tatiana Da; Castellano, Sabrina; Banfi, Elena; Prato, Maurizio

doi:10.1016/s0960-894x(00)00159-1

Cited by 124 publications

(72 citation statements)

References 12 publications

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“…In addition, the strong inter individual differences in reactivity towards the two fullerenes have to be taken into account. In the present study, PBMC from three individuals (no 7,8,9) were preferentially influenced, however we did not notice any difference between these three and the remaining 27 other individuals with respect to allergic disposition, prior vaccinations, or infectious disorders (not shown). Of course, one has to keep in mind that individual reactivity is influenced by the personal momentary milieu including nutritional status, physical exercise, infections, and psychological stress.…”

contrasting

confidence: 64%

“…3 was observed with PBMC from five individuals (no 7,8,9,13,20). This increase was statistically significant on the whole group level with poly-C60 at concentrations of 8-800 ng/mL and nepo-C60 at concentrations 0.08-800 ng/mL (Figure 1).…”

Section: Effect On Proliferative Responsementioning

confidence: 86%

“…6,7,8,12,13) and from five individuals by nepo-C60 (no. 1, 6, 7, 8, 10) resulting in significant differences as compared to the spontaneous production ( Figure 4, PBMC from three subjects already produced IL-6 spontaneously and were, therefore excluded Figure 1 The effect of varying concentrations (0.08-800 ng/mL = 0.1-100 nM) of poly-C60 and nepo-C60 on the proliferative response of PBMC from 20 healthy volunteers measured by the lymphocyte transformation test.…”

Section: Effect On Cytokine Secretionmentioning

confidence: 99%

“…7 It also enables C60 to intercalate into biological membranes and destabilize them, which would explain its antibacterial activity. 4,[8][9][10][11] On the other hand, their poor solubility in polar solutions and their high potential to form aggregates has several negative aspects, which have, however, been overcome by different methods. Thus, fullerenes can be functionalized and derived in multiple ways in order to enhance their hydrophilicity (eg, coupling with -OH, -COOH, or -NH 2 ).…”

Section: Introductionmentioning

confidence: 99%

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Effect of buckminsterfullerenes on cells of the innate and adaptive immune system: an in vitro study with human peripheral blood mononuclear cells

Bunz¹,

Plankenhorn²,

Klein³

2012

IJN

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C60 nanoparticles, the so-called buckminsterfullerenes, have attracted great attention for medical applications as carriers, enzyme inhibitors or radical scavengers. However, publications evaluating their immunological mechanisms are still rather limited.Therefore, we aimed to analyze systematically the in vitro influence of polyhydroxy-C60 (poly-C60) and N-ethyl-polyamino-C60 (nepo-C60) on peripheral blood mononuclear cells (PBMC) from healthy individuals, angling their effect on proliferation, expression of surface markers, and cytokine production. We isolated PBMC from 20 healthy subjects and incubated them in a first step only with poly-C60 or nepo-C60, and in a second step together with recall antigens (purified protein derivative, tetanus toxoid, bacillus Calmette-Guérin). Proliferation was determined by 3 H-thymidine incorporation, activation of PBMC-subpopulations by flow cytometry by measurement of the activation marker CD69, and secretion of T helper cell type 1 (TH1)-(interferon-gamma [IFN-γ], tumor necrosis factor beta [TNF-β]), TH2-(interleukin-5 [IL-5], -13, -10) and macrophage/monocyte-related cytokines (IL-1, IL-6, TNF-α) into the supernatants by enzyme-linked immunosorbent assay. Both fullerenes did not influence T cell reactivity, with no enhanced expression of CD69 and production of T cell cytokines observed, the CD4/CD8 ratio remaining unaffected. In contrast, they significantly enhanced the release of IL-6 and CD69-expression by CD56 positive natural killer cells. PBMC, which had been cultured together with the three recall antigens were not affected by both fullerenes at all. These data indicate that fullerenes do not interact with T cell reactivity but may activate cells of the innate immune system. Furthermore, they seem to act only on 'naïve' cells, which have not been prestimulated with recall antigens, there are however, large inter individual differences.

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contrasting

confidence: 64%

Section: Effect On Proliferative Responsementioning

confidence: 86%

Section: Effect On Cytokine Secretionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of buckminsterfullerenes on cells of the innate and adaptive immune system: an in vitro study with human peripheral blood mononuclear cells

Bunz¹,

Plankenhorn²,

Klein³

2012

IJN

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“…Work in our laboratory (data not shown) as well as others (15,16) has shown C60 to have a limited toxicity toward Gram-negative bacteria when they are grown in cultures with concentrations of salts typical of culture media. We would also expect ionic strength to affect the physical behavior of C60 in aqueous systems (11). Combined these data suggest that bioavailability of chemicals is important in controlling their environmental impacts.…”

Section: Resultsmentioning

confidence: 95%

Impact of Fullerene (C60) on a Soil Microbial Community

Tong

Bischoff

Nies

et al. 2007

Environ. Sci. Technol.

319

212

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The nascent state of the nanoproduct industry calls for important early assessment of environmental impacts before significant releases have occurred. Clearly, the impact of manufactured nanomaterials on key soil processes must be addressed so that an unbiased discussion concerning the environmental consequences of nanotechnology can take place. In this study, soils were treated with either 1 μg C60 g-1 soil in aqueous suspension (nC60) or 1000 μg C60 g-1 soil in granular form, a control containing equivalent tetrahydrofuran residues as generated during nC60 formation process or water and incubated for up to 180 days. Treatment effects on soil respiration, both basal and glucose-induced, were evaluated. The effects on the soil microbial community size was evaluated using total phospholipid derived phosphate. The impact on community structure was evaluated using both fatty acid profiles and following extraction of total genomic DNA, by DGGE after PCR amplification of total genomic DNA using bacterial variable V3 region targeted primers. In addition, treatment affects on soil enzymatic activities for β-glucosidase, acid-phosphatase, dehydrogenase, and urease were followed. Our observations show that the introduction of fullerene, as either C60 or nC60, has little impact on the structure and function of the soil microbial community and microbial processes.

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