Antinociceptive action of the extract and the flavonoid quercitrin isolated from Bauhinia microstachya leaves

Abstract

This study examined the antinociceptive effect of Bauhinia microstachya (Leguminosae), a native plant widely distributed in the South of Brazil, in several chemical and mechanical models of pain. The methanolic extract (ME) from B. microstachya (3--30 mg kg(-1), i.p.) and the isolated compound quercitrin (1--10 mg kg(-1), i.p.), given 30 min earlier, produced a dose-dependent inhibition of acetic-acid-induced visceral pain in mice, with a mean ID50 value (dose necessary to reduce the nociceptive response by 50% relative to the control value) of 7.9 and 2.4 mg kg(-1), respectively. The ME of B. microstachya (3--100 mg kg(-1), i.p., 30 min earlier) also caused a dose-dependent inhibition of capsaicin-induced pain, with a mean ID50 value of 18.8 mg kg(-1). Moreover, the ME (3--100 mg kg(-1), i.p., 30 min earlier) produced marked inhibition of both phases of formalin-induced pain, with mean ID50 values for the neurogenic and the inflammatory phases of 30.3 and 17.2 mg kg(-1), respectively. In addition, the ME of B. microstachya (3--300 mg kg(-1), i.p., 30 min earlier) inhibited, in a graded manner, the hyperalgesia induced by bradykinin (3.2 microg/paw), substance P (13.5 microg/paw), carrageenan (300 microg/paw), capsaicin (100 microg/paw) and adrenaline (100 ng/paw) in the rat paw, with mean ID50 values of 20.5, 17.9, 101.8, 54.2 and 99.7 mg kg(-1), respectively. Taken together, these data demonstrate that ME of B. microstachya elicited a pronounced antinociceptive action against several chemical and mechanical models of pain in mice and rats. The precise mechanism responsible for the antinociceptive effect of the extract still remains unclear, but seems to be partly related to modulation of the release or action of pro-inflammatory mediators involved in the models of pain used. Finally, the flavonoid quercitrin isolated from this plant appears to contribute for the antinociceptive property of the methanolic extract.

Antihyperglycemic and antinociceptive activity evaluation of ‘Khoyer’ prepared from boiling the wood of <i>Acacia catechu</i> in water

“…It is noteworthy in this aspect that quercitrin has been reported to be present in khoyer (Rajendra et al, 2011). Antinociceptive activity as demonstrated by inhibition of acetic acid-induced visceral pain in mice has been reported for Bauhinia microstachya leaf extract as well as the isolated active component from leaf, quercitrin (Gadotti et al, 2005). Quercitrin also reportedly demonstrated significant anti-inflammatory activity against 12-Otetradecanoyl-13-acetate-induced mouse ear edema (Erdemoglu et al, 2008).…”

Phytochemicals from Phyllanthus niruri Linn. and their pharmacological properties: a review

“…Quercitrin Quercitrin (quercetin 3-O-alpha-L-rhamnopyranoside) (Figure 1) is a flavone glycoside and has anti-leishmanial, anti-nociceptive and anti-inflammatory activity (Camuesco et al 2004;Gadotti et al 2005;Muzitano et al 2006). It has demonstrated a potent anti-leishmanial activity (IC 50 approximately 1 mg mL −1 ) with a low toxicity profile (Muzitano et al 2006).…”

Antinociceptive activity of Hypericum grandifolium Choisy in mice

“…Since previous studies have shown that the flavonoids quercetin, quercitrin and hyperoside possess antinociceptive effects when tested individually [33][34][35], it is possible that the flavonoids present in the methanol extracts and butanol fraction of this species may be responsible, at least in part, for the antinociceptive effects reported here. On the other hand, since the benzophenone derivatives were detected as major compounds in the chloroform fraction and their subfractions, it can be hypothesized that these compounds may be also associated with the effects found in the present study.…”