Antinociceptive activity of aqueous extract and isolated compounds of Lithrea molleoides

Morucci, Florencia; López, Paula; Miño, Jorge; Ferraro, Graciela; Gorzalczany, Susana

doi:10.1016/j.jep.2012.05.009

Cited by 27 publications

(29 citation statements)

References 25 publications

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“…The time spent licking the paw was inhibited only by 30 mg/kg of compound 1 ( Figure 2B). This finding is in agreement with a previous study showing 1 inhibited the second phase of the test, 13 but others also found that 1 inhibits both phases of the licking time of the formalin test. 2 The first phase (0−5 min) of the formalin test depends on neurotransmitter release such as serotonin and bradykinin, molecules from resident cells such as histamine, 18 and activation of transient receptor potential ankyrin 1 (TRPA1) expressed by neurons.…”

Section: Journal Of Natural Productssupporting

confidence: 93%

“…Other studies also used 60 min of pretreatment time. 2,13 The antinociceptive efficacy of 1 was also tested by treatment via other routes of administration. Mice were treated with 1 (3−30 mg/kg, 1 h; 5% Tween 80 diluted in saline) by subcutaneous (sc) ( Figure 1C) or per oral (po) ( Figure 1D) routes, and the acetic acidinduced writhing response was assessed.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…12 Regarding inflammation and pain, oral treatment (50 mg/kg) with 1 reduces the time spent licking the paw in both phases of the formalin test. 2 On the other hand, 1 reduced only the second phase of formalin-induced paw licking when intraperitoneal treatment (100 mg/kg) was used, 13 indicating that pharmacokinetic issues modulate its activities. Compound 1 also inhibited the acetic acid-induced writhing response at doses of 3−30 mg/kg, 13 carrageenan-induced paw edema in mice, and carrageenan-induced recruitment of neutrophils to the peritoneal cavity of rats.…”

mentioning

confidence: 99%

“…2 On the other hand, 1 reduced only the second phase of formalin-induced paw licking when intraperitoneal treatment (100 mg/kg) was used, 13 indicating that pharmacokinetic issues modulate its activities. Compound 1 also inhibited the acetic acid-induced writhing response at doses of 3−30 mg/kg, 13 carrageenan-induced paw edema in mice, and carrageenan-induced recruitment of neutrophils to the peritoneal cavity of rats. 2 The nociceptive and inflammatory responses are related to pro-inflammatory mediators such as cytokines (e.g., TNF-α, IL-1β, and IL-6), which are produced by the activation of transcription factors including NFκB (nuclear factor kappalight-chain-enhancer of activated B cells).…”

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confidence: 99%

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Vanillic Acid Inhibits Inflammatory Pain by Inhibiting Neutrophil Recruitment, Oxidative Stress, Cytokine Production, and NFκB Activation in Mice

et al. 2015

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Vanillic acid (1) is a flavoring agent found in edible plants and fruits. It is an oxidized form of vanillin. Phenolic compounds form a substantial part of plant foods used as antioxidants with beneficial biological activities. These compounds have received considerable attention because of their role in preventing human diseases. Especially, 1 presents antibacterial, antimicrobial, and chemopreventive effects. However, the mechanisms by which 1 exerts its anti-inflammatory effects in vivo are incompletely understood. Thus, the effect of 1 was evaluated in murine models of inflammatory pain. Treatment with 1 inhibited the overt pain-like behavior induced by acetic acid, phenyl-p-benzoquinone, the second phase of the formalin test, and complete Freund's adjuvant (CFA). Treatment with 1 also inhibited carrageenan- and CFA-induced mechanical hyperalgesia, paw edema, myeloperoxidase activity, and N-acetyl-β-D-glucosaminidase activity. The anti-inflammatory mechanisms of 1 involved the inhibition of oxidative stress, pro-inflammatory cytokine production, and NFκB activation in the carrageenan model. The present study demonstrated 1 presents analgesic and anti-inflammatory effects in a wide range of murine inflammation models, and its mechanisms of action involves antioxidant effects and NFκB-related inhibition of pro-inflammatory cytokine production.

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Section: Journal Of Natural Productssupporting

confidence: 93%

Section: ■ Results and Discussionmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Vanillic Acid Inhibits Inflammatory Pain by Inhibiting Neutrophil Recruitment, Oxidative Stress, Cytokine Production, and NFκB Activation in Mice

et al. 2015

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“…(Anacardiaceae) was able to reduce the nociceptive effect induced by 60 acetic acid and formalin tests and this effect was partly related to the 61 presence of its main compound, vanillic acid (Morucci et al, 2012). (1 mg/kg i.p.…”

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confidence: 99%

Pharmacological mechanism underlying the antinociceptive activity of vanillic acid

Yrbas¹,

Morucci²,

Alonso³

et al. 2015

Pharmacology Biochemistry and Behavior

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Efficacy of a phenol derivative, isopropyl vanillate, as an anti‐inflammatory agent: A new small molecule inhibitor of COX and neutrophil migration

Nogueira

Souza

Oliveira

et al. 2019

Drug Development Research

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Inflammation is the response of the body to noxious stimuli such as infections, trauma, or injury. Experimental studies have shown that vanillic acid has antiinflammatory effects. The objective of this study was to investigate the antiinflammatory and antipyretic properties of the derivative of vanillic acid, isopropyl vanillate (ISP-VT), in mice. The results of this study indicated that ISP-VT reduced paw edema induced by carrageenan, dextran sulfate (DEX), compound 48/80, serotonin, bradykinin (BK), histamine (HIST), and prostaglandin E2 (PGE2). Furthermore, ISP-VT reduced recruitment of leukocytes and neutrophils and reduced its adhesion and rolling, and decreased myeloperoxidase enzyme activity (MPO), cytokine levels (tumor necrosis factor-α and interleukin-6), and vascular permeability. ISP-VT also significantly reduced the expression of cyclooxygenase-2 (COX-2) in subplantar tissue of mice. ISP-VT inhibited COX-2 selectively compared to the standard drug. Our results showed that although ISP-VT binds to COX-1, it is less toxic than indomethacin, as evidenced by MPO analysis of gastric tissue. Treatment with the ISP-VT significantly reduced rectal temperature in yeast-induced hyperthermia in mice. Our results showed that the main mechanism ISP-VT-induced anti-inflammatory activity is by inhibition of COX-2. In conclusion, our results indicate that ISP-VT has potential as an anti-inflammatory and antipyretic therapeutic compound. K E Y W O R D Santipyretic, COX-2 inhibition, inflammation, vanillic acid

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Antinociceptive activity of aqueous extract and isolated compounds of Lithrea molleoides

Cited by 27 publications

References 25 publications

Vanillic Acid Inhibits Inflammatory Pain by Inhibiting Neutrophil Recruitment, Oxidative Stress, Cytokine Production, and NFκB Activation in Mice

Vanillic Acid Inhibits Inflammatory Pain by Inhibiting Neutrophil Recruitment, Oxidative Stress, Cytokine Production, and NFκB Activation in Mice

Pharmacological mechanism underlying the antinociceptive activity of vanillic acid

Efficacy of a phenol derivative, isopropyl vanillate, as an anti‐inflammatory agent: A new small molecule inhibitor of COX and neutrophil migration

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