Antinociceptive and Anti-inflammatory Activities of Lectin from Marine Red Alga Pterocladiella capillacea

Abstract

Researchers see algae as a promising tool to discover both efficient and safe agents for pain therapy. We evaluated the antinociceptive and anti-inflammatory activities of lectin from the marine alga Pterocladiella capillacea lectin (PcL). PcL was purified and tested in classical models of nociception and inflammation. Male Swiss mice received PcL 30 min prior to receiving 0.8% acetic acid (10 m ml/10 g, i.p.), 1% formalin (20 m ml/intraplantar) or the hot plate test, and were compared to untreated animals or animals pretreated with indomethacin or morphine. PcL (0.9, 8.1 or 72.9 mg/kg, i.v.) significantly reduced the number of writhes (30%, 39%, and 52%, respectively). PcL (72.9 mg/kg, i.v.) also reduced (pϽ Ͻ0.05) both the first and second phases of the formalin test by 58% and 87%, respectively. However, PcL (72.9 mg/kg) did not present significant antinociceptive effects in the hot plate test when compared to morphine, suggesting that its antinociceptive action occurs via peripheral rather than a central-acting mechanism. It was also observed that leukocyte migration was induced by carrageenan (500 m mg/cavity) in male Wistar rats and that PcL (8.1 mg/kg, i.v.) significantly reduced neutrophil migration by 84%, as compared to untreated animals, suggesting inhibition of inflammatory mediators. The data indicated that PcL has peripheral actions with both anti-inflammatory and antinociceptive properties.

Biological Applications of Plants and Algae Lectins: An Overview

“…The data indicated that HCA, PcL and CcL have actions antiinflammatory and antinociceptive (in formalin and acetic acid models). However, these lectins did not present significant antinociceptive effects in the hot plate test [110,111,112].…”

Synthesis and Anti-Inflammatory Activity Evaluation of 5-(1-Benzyl-1H-[1,2,3]Triazol-4-yl)-4-Phenyl- 4H-[1,2,4]Triazole-3-Thiol Derivatives

“…After 10 h, solvent was distilled off under reduced pressure to give 23 (82%) yield. 19 1-Benzyl-1H- [1,2,3]triazole-4-carboxylic acid methyl ester (23, 12 g, 0.055 mol) in anhydrous ethanol (50 ml), hydrazine hydrate (80%, 20 mL) was added and refluxed (80℃). After 48 h, solvent was distilled off under reduced pressure, the precipitate was filtered and recrystallized from methanol and petroleum ether to give to 22 (80%) yield.…”

“…[11][12][13]16 In order to search new compounds with higher anti-inflammatory activities and lower toxicity, 1,2,3-triazole and 1,2,4-triazole derivatives were designed on the basis of association principle. All of these 5-(1-Benzyl-1H- [1,2,3]triazol-4-yl)-4-phenyl-4H- [1,2,4] triazole-3-thiol derivatives were synthesized, and then the anti-inflammatory activities (in vitro) were evaluated.…”

Lectin isolated from Brazilian seeds of velvet bean (Mucuna pruriens (L) DC.) presents analgesic, anti-inflammatory and antihemolytic action

“…The referred experimental model has been widely applied to verify analgesic activity promoted by lectins present in vegetal species (Holanda et al, 2009) and algae , as observed in a lectin-like protein purified from Clitoria fairchildiana seeds, in which dose-dependent antinociceptive activity was also reported (Leite et al, 2012). The lectinic site seems to be involved significantly on the lectin-promoted antinociceptive activity, as reported in the analgesic agglutinins isolated from C. boliviana seeds and the red algae Pterocladiella capilacea, in which previous association of these lectins with their specific carbohydrates (glycose and mucin) blocked the protein from exert its function against the painful stimulus, resulting in no contortion inhibition (Figueiredo et al, 2009;Silva et al, 2010).…”