Antinociceptive and wound healing activities of Croton adamantinus Müll. Arg. essential oil

Abstract

Leaves of Croton adamantinus have been used to treat inflammation and skin wounds in the semi-arid area of the Northeast of Brazil. In order to evaluate if the essential oil (EO) was responsible for the claimed activities; antinociceptive, wound healing and antimicrobial tests were carried out. Twenty constituents were identified in C. adamantinus EO by GC-MS, ¹H-NMR and ¹³C-NMR, the major compounds being methyl-eugenol (14.81%) and 1,8-cineol (13.74%). Antinociceptive activity was evaluated by the formalin test and the abdominal contortion assay in mice. The EO (50 and 100 mg/kg) decreased the licking time of both phases of the formalin test when compared to the vehicle, but not to morphine (7.5 mg/kg). In the abdominal contortion assay, the EO (50 and 100 mg/kg) reduced the number of contortions compared to the vehicle and to indometacin (10 mg/kg). The wound healing activity was verified also using two experimental models: excisional wound and dead space. Topical treatment with the EO (1%) increased the wound contraction from the third day of treatment (compared with nitrofurazone 0.2%), while systemic treatment (50 mg/kg/day) increased granulation tissue formation and reduced the water content. C. adamantinus EO also showed antimicrobial activity against Staphylococcus aureus in disk diffusion method. These results corroborate the ethnobotanical use of this specie by Brazilian population.

The power of odour volatiles from unifloral melipona honey evaluated by gas chromatography–olfactometry Osme techniques

“…The volatile profile in Table indicates that the ‘velame branco’ honey contained methyl eugenol, a compound that typically provides a ‘clove’, ‘spicy’ and ‘hot’ aroma (http://www.odour.org.uk). Many species of the same genus of ‘velame branco’, such as Croton adamantinus , Croton zehntneri , and Croton malambo , include methyl eugenol as one of the main chemical constituents of their essential oils . The acids in honeys have different aromas that range from spicy to rancid, depending on the length of the carbon chain .…”

Eucalyptol induces hyperexcitability and epileptiform activity in snail neurons by inhibiting potassium channels

“…It has also a broad range of applications in therapeutics and especially used in the folk medicine as a remedy for respiratory problems because of its antiseptic, decongestant and expectorant properties (Juergens et al, 2003). Eucalyptol reduces inflammation and pain when applied topically (Ximenes et al, 2013). The agoniztic action of this compound on TRPM8 channels underlies its cooling action.…”

Antinociceptive Effect of the Essential Oil Obtained from the Leaves ofCroton cordiifoliusBaill. (Euphorbiaceae) in Mice

“…The acetic acid induced writhing was performed according to the protocol described by Koster et al [ 19 ] with some modifications [ 8 ]. C. cordiifolius essential oil (50 and 100 mg/kg, i.p.)…”

“…Many pharmacological activities are related to Croton spp., such as antinociceptive, anti-inflammatory, wound healing, anticancer, and antimicrobial activities [ 3 – 8 ]. However, the pharmacological potential of many species remains completely unexplored.…”