Antinociceptive effect and mechanism of supercritical carbon dioxide extract of Aloysia gratissima leaves in mice

Souza, Maryelen Alijar; Scapinello, Jaqueline; Guzatti, João G.G.; Scatolin, Mikaela; Martello, Rafael; Schindler, Monica Santin Zanatta; Calisto, Jean Felipe Fossá; Alves, Bianca Oliveira; Morgan, Letícia Vidor; Oliveira, J. Vladimir; Magro, Jacir Dal; Müller, Liz Girardi

doi:10.1016/j.bj.2020.06.009

Cited by 6 publications

(12 citation statements)

References 46 publications

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“…S2). In addition, indomethacin was selected over other analgesic drugs because it did not affect locomotor activity and motor coordination 66 as well as its prominent anti-inflammatory activity. As shown in this study, indomethacin did not affect the general behavior of mice in the automated home-cage LABORAS (Fig.…”

Section: Discussionmentioning

confidence: 99%

Automated home-cage for the evaluation of innate non-reflexive pain behaviors in a mouse model of inflammatory pain

Hasriadi

Wasana

Vajragupta

et al. 2021

Sci Rep

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The failure to develop analgesic drugs is attributed not only to the complex and diverse pathophysiology of pain in humans but also to the poor experimental design and poor preclinical assessment of pain. Although considerable efforts have been devoted to overcoming the relevant problems, many features of the behavioral pain assessment remain to be characterized. For example, a decreased locomotor activity as a common presentation of pain-like behavior has yet to be described. Studies on mice experimentally induced with carrageenan have provided opportunities to explore pain-related behaviors in automated home-cage monitoring. Through this approach, the locomotor activities of mice with carrageenan-induced inflammatory pain can be precisely and objectively captured. Here, we found that the mobile behaviors of mice reduced, and their immobility increased, indicating that carrageenan induction in mice caused a significant decrease in locomotor activity. These non-reflexive pain behaviors were strongly correlated with the reflexive pain behaviors measured via von Frey and plantar tests. Furthermore, the pharmacological intervention using indomethacin improved the locomotor activity of mice with carrageenan-induced pain. Thus, the analysis of the locomotor activity in automated home-cage monitoring is useful for studying the behavioral analgesia and the pharmacological screening of analgesic drugs. The combined evaluation of reflexive and non-reflexive pain behaviors enhances the translational utility of preclinical pain research in rodents.

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Section: Discussionmentioning

confidence: 99%

Automated home-cage for the evaluation of innate non-reflexive pain behaviors in a mouse model of inflammatory pain

Hasriadi

Wasana

Vajragupta

et al. 2021

Sci Rep

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“…It was noticed that articles about A. gratissima are associated with in vitro and in vivo assays. Biological properties of this species, such as virucidal [ 22 ], nematicide [ 33 ], antioxidant [ 29 ], sedative/anesthetic [ 6 ], antidepressant-like neuroprotective [ 14 ], antibacterial [ 28 ], antinociceptive, anti-inflammatory [ 13 , 26 ], and antispasmodic [ 18 ], have been reported. There are no clinical studies on A. gratissima in the literature.…”

Section: Resultsmentioning

confidence: 99%

“…However, there are a few articles that carried out the supercritical fluid extraction of the plant. Souza et al [ 13 , 26 ] found volatile terpenic compounds in the extract of A. gratissima leaves obtained by supercritical fluid with carbon dioxide (SCCO 2 ). The main compounds found in the supercritical extract were guaiol (18.50%) and pinocamphone (11.40%), in addition to the presence of other compounds such as (-)trans-pinocarvyl acetate (10.50%), b-cubeben (8.21%), caryophyllene (7.63%), γ -elemene (7.28%), caryophyllene oxide (6.76%), (-)-spatulenol (6.17%), pinocarvil acetate (5.31%), bunesol (4.67%), isopinocamphone (3.52%), myrtenol (3.24%), and humelene (2.97%) in smaller amounts.…”

Section: Resultsmentioning

confidence: 99%

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Biological Properties of Aloysia gratissima (Gillies & Hook.) Tronc. (Verbenaceae)

Souza

Petry

Morgan

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Aloysia gratissima is a plant native to America, with applications in folk medicine for a wide range of diseases, such as bronchial infections, lung disorders, nervous system disorders (depression, anxiety), and inflammatory processes, among others. However, investigations about this species and its biological actions are still scarce. This literature review was carried out using articles published in the past 30 years on the PubMed, SciELO, and Web of Science platforms, with the focus on the method of extraction, chemical composition, and clinical and preclinical studies on the pharmacological properties of A. gratissima. We noticed that the main constituents of A. gratissima are guaiol, pinocamphone, ß-pinene, and 1,8-cineole. Additionally, preclinical studies reveal that A. gratissima extracts present antidepressant, anti-inflammatory, antinociceptive, antibacterial, antifungal, and virucidal effects. The results also demonstrate that there is a greater interest on the part of researchers from 2012 onwards in studying A. gratissima extracts with potential for possible new drugs.

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“…The team subjected A. gratissima leaves to extraction with supercritical CO 2 and proved that the extract exerts an antinociceptive effect, mediated by ATP sensitive K + channels. They suggest this effect to be caused by terpene compounds in the leaves [ 23 ].…”

Section: Also In This Issuementioning

confidence: 99%

One day at a time

Kattner¹

2021

Biomedical Journal

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In this issue of Biomedical Journal we get to know measures to prevent a nosocomial COVID-19 outbreak, a compound that is able to stall SARS-CoV-2 replication, and the connection between air pollution and COVID-19 cases. Another article allows an insight into the potential of treating HIV combining a conventional drug and low level laser therapy. Furthermore, the advantages of awake craniotomy are presented, the efficacy of IRES is examined, and plant extracts are on the one hand explored as a nociceptive agent and on the other hand as therapeutic approach against breast cancer. We learn about drug resistance in liver cancer, a mutation involved in a rare inflammatory disorder, and lung surgery related unilateral vocal fold paralysis. Finally, the success of emergency endotracheal intubations across different hospital units is compared, the importance of monitoring cerebral blood flow in asphyxiated neonates is elucidated, and resistance variants in hepatitis C virus are examined. A study about the necessity to perform quantitative cardiac MRI in Asian population is presented, and an approach is shown on how to augment the effect of platelet-rich plasma injections in chronic knee osteoarthritis.

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Antinociceptive effect and mechanism of supercritical carbon dioxide extract of Aloysia gratissima leaves in mice

Cited by 6 publications

References 46 publications

Automated home-cage for the evaluation of innate non-reflexive pain behaviors in a mouse model of inflammatory pain

Automated home-cage for the evaluation of innate non-reflexive pain behaviors in a mouse model of inflammatory pain

Biological Properties of Aloysia gratissima (Gillies & Hook.) Tronc. (Verbenaceae)

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