Antioxidant, ACE inhibitory, and acetylcholinesterase inhibitory activities of subcritical water extract of blue mussel

Han, Ji-Kyoung; Sung, Su-Chang; Jo, Min-Ji; Lee, Seung‐Cheol

doi:10.1007/s10068-018-0319-z

Cited by 6 publications

(3 citation statements)

References 18 publications

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“…AJuly19, KHS19 and KHO19 displayed AChE inhibition (Figure 8), though the % inhibition (KHO19: 29.59% ± 1.88%) of the samples (1 mg/mL) was low as compared to positive control acotiamide dihydrochloride (94.201% ± 0.89%) assayed at 100 uM concentration (IC 50 3 uM). Researchers have found AChE, ACE-I and DPPH inhibitory activity in subcritical extracts of blue mussels [47]. The obtained results warrant further studies to explore the possible potential of some of these hydrolysates in promoting mental health.…”

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confidence: 83%

Characterisation of Seasonal Mytilus edulis By-Products and Generation of Bioactive Hydrolysates

2020

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Mussel cultivation results in tons of by-product, with 27% of the harvest considered as reject material. In this study, mussel by-products considered to be undersized (mussels with a cooked meat yield <30%), mussels with broken shells and barnacle-fouled mussels were collected from three different locations in the west, north-west and south-west of Ireland. Samples were hydrolysed using controlled temperatures and agitation, and the proteolytic enzyme Protamex® was added at an enzyme:substrate ratio of 1:50 (w:v). The hydrolysates were freeze-dried and analysed for protein content and amino acid composition, lipid content and fatty acid methyl ester (FAME) composition, ash and techno-functional and bioactive activities. The degree of hydrolysis was determined using the Adler-Nissen pH stat method and was found to be between 2.41% ± 0% and 7.55 ± 0.6 %. Mussel by-products harvested between February and May 2019 had protein contents ranging from 36.76% ± 0.41% to 52.19% ± 1.78%. The protein content of mussels collected from July to October (the spawning season) ranged from 59.07% ± 1.375% to 68.31% ± 3.42%. The ratio of essential to nonessential amino acids varied from 0.68–0.96 and it was highest for a sample collected in November from the west of Ireland. All the hydrolysate samples contained omega-3 polyunsaturated fatty acids (PUFA), especially eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), which are known anti-inflammatory agents. Selected hydrolysates which had angiotensin-converting enzyme I (ACE-I; EC 3.4.15.1) and dipeptidyl peptidase IV (DPP-IV; EC 3.4.14.5) inhibitory activities were filtered using 3-kDa membrane filtration and the permeate fraction was sequenced using mass spectrometry (MS). Identified peptides were >7 amino acids in length. Following BIOPEP database mining, 91% of the by-product mussel peptides identified were found to be previously identified DPP-IV and ACE-I inhibitory peptides, and this was confirmed using in vitro bioassays. The ACE-I inhibitory activity of the by-product mussel hydrolysates ranged from 22.23% ± 1.79% to 86.08% ± 1.59% and the most active hydrolysate had an ACE-I inhibitory concentration (IC50) value of 0.2944 mg/mL compared to the positive control, captopril. This work demonstrates that by-product mussel hydrolysates have potential for use as health-promoting ingredients.

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confidence: 83%

Characterisation of Seasonal Mytilus edulis By-Products and Generation of Bioactive Hydrolysates

2020

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“…SCW can also break down covalent bonds such as ester, ether, and peptide bonds, making it advantageous for the extraction of useful materials (Kus, 2012). By using SCW, materials maintaining physiological activity can be extracted from sources such as watermelons (Kim et al, 2014a), oyster mushrooms (Jo et al, 2013), blue mussel (Han et al, 2018), and Styela clava (Jo et al, 2018). Therefore, in this study, we present the possibility of applying SCW to PBL by manufacturing the extract under three different temperatures and analyzing the physiological activity of alcohol metabolizing enzymatic activity, angiotensin converting enzyme (ACE) inhibition, and tyrosinase inhibition.…”

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confidence: 99%

Effect of subcritical water extraction conditions on the activity of alcohol metabolizing enzymes, ACE inhibition, and tyrosinase inhibition in Protaetia brevitarsis larvae

Bae

Lee

2020

Food Sci Biotechnol

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In order to develop processing methods with high physiological activity for Protaetia brevitarsis larvae (PBL), subcritical water (SCW) extraction was applied. The dried powder (1 g) of PBL was extracted with 10 mL distilled water at 100, 200, and 300 °C for 30 min. The SCW treatment significantly (p \ 0.05) increased some physiological activities of the PBL extracts. The SCW extract at 300 °C increased alcohol dehydrogenase, acetaldehyde dehydrogenase, and tyrosinase inhibitory activities from 192.3 ± 4.1% to 452.2 ± 0.5%, 125.4 ± 2.9% to 153.3 ± 0.4%, and -7.0 ± 0.7% to 26.1 ± 1.4%, respectively, compared to the extract at 100 °C. Contrarily, the inhibition activity of angiotensin converting enzyme was the highest at 200 °C. These results suggest that SCW is a suitable method to extract and maintain the physiological activity of PBL.

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“…in various solvents that are allowed in the food industry, namely, distilled water (DW), hot DW, subcritical water, and ethanol. Subcritical water extraction (SWE), which is a process featuring high temperatures and pressure (Han, Sung, Jo, & Lee, 2018), permits the selective extraction of different grades of compounds depending on the temperature used (Rodriguez-Meizoso et al, 2006;Singh & Saldaña, 2011). The advantages of this process are its environmental-friendliness, production of high-quality extracts, low cost, and shortened extraction time (Dai & Mumper, 2010;Gong et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Antithrombotic Effect of Artemisia princeps Pampanini Extracts in Vitro and in FeCl₃‐Induced Thrombosis Rats

Kim

Seok

et al. 2019

Journal of Food Science

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Extracts of several plants possess antithrombotic effects. Herein, we examined the antithrombotic effects of different extracts of Artemisia princeps Pampanini prepared using distilled water, hot distilled water, 70% ethanol, or subcritical water. The antithrombotic effects were determined using a co‐culture system consisting of tumor necrosis factor‐alpha (TNF‐α)‐treated EA.hy926 cells and THP‐1 cells. In addition, the coagulation time of plasma collected from healthy volunteers was evaluated in terms of the prothrombin time and activated partial thromboplastin time. A carotid arterial thrombosis model was induced by ferric chloride in Sprague Dawley rats. The rats were treated with either sterile water or three different doses of the subcritical water extract for 2 weeks. The thrombus weight, gene expression of cell adhesion molecules, and histological characteristics were assessed. The results of in vitro studies revealed a significant inhibition in the adhesion of monocytes to EA.hy926 cells stimulated by TNF‐α in the subcritical water extract‐treated group. We also observed considerable suppression of the occlusion and mRNA expression of cell adhesion molecules in the in vivo experiments. This study suggests that Artemisia princeps Pampanini may have the potential to improve blood coagulation.

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Antioxidant, ACE inhibitory, and acetylcholinesterase inhibitory activities of subcritical water extract of blue mussel

Cited by 6 publications

References 18 publications

Characterisation of Seasonal Mytilus edulis By-Products and Generation of Bioactive Hydrolysates

Characterisation of Seasonal Mytilus edulis By-Products and Generation of Bioactive Hydrolysates

Effect of subcritical water extraction conditions on the activity of alcohol metabolizing enzymes, ACE inhibition, and tyrosinase inhibition in Protaetia brevitarsis larvae

Antithrombotic Effect of Artemisia princeps Pampanini Extracts in Vitro and in FeCl₃‐Induced Thrombosis Rats

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Antioxidant, ACE inhibitory, and acetylcholinesterase inhibitory activities of subcritical water extract of blue mussel

Cited by 6 publications

References 18 publications

Characterisation of Seasonal Mytilus edulis By-Products and Generation of Bioactive Hydrolysates

Characterisation of Seasonal Mytilus edulis By-Products and Generation of Bioactive Hydrolysates

Effect of subcritical water extraction conditions on the activity of alcohol metabolizing enzymes, ACE inhibition, and tyrosinase inhibition in Protaetia brevitarsis larvae

Antithrombotic Effect of Artemisia princeps Pampanini Extracts in Vitro and in FeCl3‐Induced Thrombosis Rats

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Antithrombotic Effect of Artemisia princeps Pampanini Extracts in Vitro and in FeCl₃‐Induced Thrombosis Rats