Antioxidant Activities and Total Phenolics of Acacia Honey

Abstract

Abstract:The antioxidant activities and total phenolic content of 30 samples of acacia honey from Croatian territory were analysed. Phenolics were determined by the modified Folin-Ciocalteu method, antiradical activity by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method and potential antioxidant activity using the ferric reducing antioxidant power (FRAP) method. In all samples, physicochemical parameters (water content, electrical conductivity, total reducing sugars, sucrose content, acidity, hydroxymethylfurfural content, prolin content, optical rotation, diastase activity and invertase activity) were measured according to Croatian legislation and International regulatory standards. Honey can be considered as a dietary supplement as it contains some important components including α-tocopherol, ascorbic acid, flavonoids and phenolics. The composition and properties of honey are dependent on floral origins, climatic conditions of the produced area, processing and storage methods. The results of physicochemical analyses showed that all the values of investigated parameters are in agreement with the current legislation. Phenolic content ranged from 31.72 mg/kg to 80.11 mg/kg, antiradical activity expressed as IC 50 ranged from 61.28% to 253.47% and antioxidant activity expressed as FRAP value from 6.95 to 142.43. A positive correlation was observed between total phenolic content and antioxidant activity, indicating that phenolic compounds are mainly responsible for the antioxidant power of acacia honey.

The Antioxidant Activity of Maltese Honey

“…However, a study by Khalil and et al (2012) found a low correlation between phenols and reducing power using the FRAP assay. Others have reported strong correlations between the TP and FRAP assay results: r = 0.885 (Beretta et al, 2005), r = 0.88 (Krpan et al, 2009), and r = 0.873 (Piljac-Žegarac et al, 2009), in agreement with our findings (r TP/AAE-RedPw = 0.894). Nevertheless, these authors found a strong correlation between TP and the DPPH assay and a high correlation between the two assays with r>0.8 compared with r AAE-RedPw/AAE-DPPH = 0.597 in this study.…”

“…Nevertheless, these authors found a strong correlation between TP and the DPPH assay and a high correlation between the two assays with r>0.8 compared with r AAE-RedPw/AAE-DPPH = 0.597 in this study. Thus, the studies by Beretta and et al (2005), Krpan andPiljac-Žegarac and suggest that phenols are the major components that are responsible for the reducing ability and radical scavenging properties of honey. TP and TF were weakly correlated (r TP/TF = 0.008).…”

Preponderance of Bioactive Medicinal Compounds and ATR-FTIR Spectroscopy of Coriander and Mustard Floral Honey from <i>Apis mellifera</i>

“…The amount of total phenolic was calculated by using the standard curve ( Fig. 1) of gallic acid drawn inside a concentration range of 8.0 x 10 -4 to 4.0 x 10 -3 mg mL -1 having R 2 value 0.994 and was expressed as mg gallic acid equivalents kg -1 (mg GAE kg -1 ) honey [11][12]15,22]. …”

“…Studies demonstrate that number of fruits, leaves, medicinal and aromatic herbs, and of some plants biosynthetic chemicals possessing antioxidant activity and may be used as a natural source of free radical scavenging compounds [10]. The majority of these plants flowers are used by the honey bees to collect nectar; consequently, floral origin bioactive components can be transferred to honey [11][12]. It is reported that the antioxidant activity and composition of honey depend on the floral source of nectar collected by the honeybee.…”

El Rol de la Miel en los Procesos Morfofisiológicos de Reparación de Heridas

“…La miel de Manuka de New Zealand presenta un gran contenido de compuestos fenólicos existiendo una fuerte correlación entre la actividad anti-oxidante y el contenido de fenoles totales (Alzahrani et al, 2012). Resultados similares fueron observados con cuatro tipos de mieles monoflorales recogidas en la región oriental de Rumania (Dobre et al, 2010) y otros lugares como la miel de Acacia recolectada en Croacia (Krpan et al, 2009). Por su parte, Pontis et al (2014) encontraron una correlación positiva entre el color y contenido de flavonoides con la capacidad antioxidante de diez muestras de miel multifloral del estado de Roraima, Brasil.…”