Antioxidant Activities of Curcumin to Mda Blood Serum Concentration and Lead Levels in Liver of Mice

Sugiharto, S; Darmanto, Win; Wahyuningsih, Sri Puji Astuti; Hayati, Alfiah; Martha, Yani; Putri, Olivia; Putri, Kevita

doi:10.22452/mjs.sp2019no3.3

Cited by 3 publications

(4 citation statements)

References 8 publications

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“…Such conditions could damage kidney cells. 15,5 The decrease in the body's antioxidants and the increase in free radicals will cause oxidation and escalate lipid peroxidation. Unstable lipid peroxidation can damage three types of compounds that significantly maintain cell integrity; they are cell membranes, proteins, and DNA.…”

Section: Necrosis Normal Cellsmentioning

confidence: 99%

“…As a result, cellular reactions do not work perfectly and could become reversible and irreversible (cell death). 5,16 Degenerative cells could have the adaptability to reach new conditions and maintain their viability. To a certain extent, the cells return to a stable state that enables them to divide by responding to injury through hypertrophy.…”

Section: Necrosis Normal Cellsmentioning

confidence: 99%

“…10 If the stress is severe or persistent, irreversible injury occurs, and the cells will die (necrosis). 5 The provision of IR Bagendit rice leaf infusion could reduce the degeneration of renal tubule cells.…”

Section: Necrosis Normal Cellsmentioning

confidence: 99%

“…An imbalance number between free radicals and antioxidants will lead to oxidative stress. 4,5 Oxidative stress is the excessive production of reactive oxygen species (ROS), such as hydroxyl, superoxide, and hydroperoxyl, which react with fats, proteins, and cellular nucleic acids; this condition results in local damage (glomerulus dysfunction) and renal tubular cells. 6,7,8 Acute PB poisoning (Pb levels in the blood >80-100 μg/dL) disrupts the structure and functions of the renal tubules.…”

Section: Introductionmentioning

confidence: 99%

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The effects of IR-Bagendit rice leaf infusions from Blora on renal tubular degeneration and necrosis

Santosa

Arif

Mukaromah

2022

J. Teknol. Laboratorium

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Plumbum exposure still becomes a health problem in Indonesia. Plumbum (Pb) is widely used in various industries; thus, it can cause health problems, including impaired kidney functions. The accumulation of Pb rise to degeneration and necrosis of renal tubule cells. Therefore, preventive efforts are necessary to inhibit renal function deterioration. Infused extract of IR Bagendit rice leaf from Blora contains protein metallothionein which could bind PB covalently. This study aims to determine the supplementation of IR Bagendit rice leaf infusion to inhibit degeneration and necrosis of renal tubular cells exposed to Pb. The experimental research method was conducted using Wistar albino rats divided into five groups: negative control group, positive group, and treatment groups 1, 2, and 3. Each group consisted of six rats. The negative control group only received a placebo, and the positive group only received Pb acetate 60 mg/kg BW/day. Meanwhile, treatment groups 1, 2, and 3 received 0.2 ml, 0.4 ml, and 0.8 ml IR Bagendit rice leaf infusions successively and Pb acetate 60 mg/kg BW/day. The experiment was conducted for 60 days. Histological preparations of tubular cells were colored using hematoxylin-eosin. Normal cell differences, degeneration, and necrosis of each group were tested using Kruskal Wallis and Mann Whitney tests.This study has revealed that the average normal cells in the positive control group are lower than those in the negative control group and treatment groups 1, 2, and 3. This difference is insignificant with the p = 0.477. Moreover, treatment groups 1, 2, and 3 show decreased cell degeneration and necrosis with significant differences of p = 0.000 and 0.001. IR Bagendit rice leaf infusions could inhibit degeneration and necrosis of renal tubular cells exposed to Pb.

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Section: Necrosis Normal Cellsmentioning

confidence: 99%

Section: Necrosis Normal Cellsmentioning

confidence: 99%

Section: Necrosis Normal Cellsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The effects of IR-Bagendit rice leaf infusions from Blora on renal tubular degeneration and necrosis

Santosa

Arif

Mukaromah

2022

J. Teknol. Laboratorium

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Biological Properties of Gynura procumbens Leaves Extract to MDA Levels and Antioxidant Activities in Liver of Mice

Sugiharto

Wibowo

Zubaidah

et al. 2022

RJPT

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The objective of our research was to the evaluated biological activities of G. procumbens leaves methanol extract (GLME) for the hepatoprotective against cadmium (Cd) toxicity in mice. Research was performed using twenty five healthy male mice, which were grouped into five treatments: P1 (control), P2 (Cd-100mg/L), P3 (GLME-100mg/L+Cd-100mg/L), P4 (GLME-200mg/L+Cd-100mg/L), P5 (GLME-300mg/L+Cd-100mg/L). The results showed GLME contain phenolic and flavonoids compound by total phenolic content (TPC) and total flavonoid content (TFC) assay, they were strongly correlated with antioxidant activities. In this study, it was also known that Cd exposure increasing malondialdehyde (MDA) level and decreasing of superoxide dismutase (SOD) and catalase (CAT) activities in liver homogenates compared to control significantly. This is in line with a decreased in the number of normal cells and slightly an increased in damage cells in the histological hepatic cells. Administration of GLME can prevent liver cell damage due to Cd treatment by increasing the number of histological normal cells and the activities of SOD and CAT enzyme and reducing the level of MDA in liver homogenates. The best treatment of GLME was 100mg/L.

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Potential of Jahe Merah (Zingiber officinale) and Temu Kunci (Boesenbergia rotunda): As Stimulator of Antioxidant Enzyme Activity and Anticancer in HepG2 Cell Line

Sugiharto,

Faradila,

Rizqi Aningrum

et al. 2024

RJPT

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The rhizomes of jahe merah (Zingiber officinale) and temu kunci (Boesenbergia rotunda) contain lots of flavonoids and phenolics. So far, Indonesian people have used Z. officinale and B. rotunda as a mixture of cooking ingredients and as traditional medicine that are known as "jamu". This research was conducted to determine the potential antioxidant activity rhizome extracts of Z. officinale (Zo), B. rotunda (Br) and their combination (Zo+Br) in mice exposed to Pb. Furthermore, it is also to determine the anticancer activity in the hepatocyte carcinoma cell line (HepG2). Twenty-five male mice were to divide into 5 treatment groups, namely P1 = control, P2 = Pb 100mg/mL, P3 = Zo 100mg/mL + Pb 100mg/mL, P4 = Br 100mg/mL + Pb 100mg/mL, P5 = combination Zo+Br (ratio 1:1) 100mg/mL + Pb 100mg/mL. Treatment was given for 30 days and on the last day the mice were anesthetized and sacrificed. Their livers were taken to observe histologically changes in hepatocyte morphology, MDA levels, as well as the activity of the antioxidant enzymes SOD and CAT. In vitro antioxidant activity was also tested using the DPPH assay and anticancer activity was tested on HepG2 cells. The results showed that range of antioxidant activity was in the strong to very strong because it has an IC50 value of Zo = 47μg/mL, Br = 67μg/mL and their combination (Zo+Br) = 100μg/mL. Treatment using Zo, Br and their combination can also prevent a significant increase in MDA levels and also significant decrease in SOD and CAT enzyme activity due to Pb administration. This is in line with the increase in normal cells and decrease in abnormal hepatocyte cells. In addition, administration of Zo, Br and their combination can inhibit the proliferation of HepG2 cells in the moderate cytotoxic range (IC50 value ranges from 42.97 to 86.94µg/mL). The conclusion of this research showed Z. officinale, B. rotunda and their combination have potential as antioxidants and can be used as drug candidates to inhibit the growth of cancer cells.

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Antioxidant Activities of Curcumin to Mda Blood Serum Concentration and Lead Levels in Liver of Mice

Cited by 3 publications

References 8 publications

The effects of IR-Bagendit rice leaf infusions from Blora on renal tubular degeneration and necrosis

The effects of IR-Bagendit rice leaf infusions from Blora on renal tubular degeneration and necrosis

Biological Properties of Gynura procumbens Leaves Extract to MDA Levels and Antioxidant Activities in Liver of Mice

Potential of Jahe Merah (Zingiber officinale) and Temu Kunci (Boesenbergia rotunda): As Stimulator of Antioxidant Enzyme Activity and Anticancer in HepG2 Cell Line

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